裸鼠结肠癌肝转移模型的建立

目的 探讨建立裸鼠结肠癌肝转移模型的方法.方法 45只裸鼠随机分为三组,分别用门静脉注射法,脾脏注射法和肝脏注射法建立结肠癌肝转移模型,并比较三种肝转移瘤模型在结肠癌肝脏转移率和自然生存期.结果 门静脉注射组,脾脏注射组和肝脏注射组肝脏转移率分别为100%(15/15),60%(9/15),100%(15/15);自然生存期分别为41.47±2.35 d,042.69±2.13 d,41.31±2.55 d.结论 门静脉注射法建立裸鼠结肠癌肝转移模型是一种简便、有效的方法.     

全氟辛烷磺酸对大鼠仔鼠学习记忆能力的影响

Objective To study the effects of prenatal and postnatal perfluorooetane sulfonate (PFOS) exposure on spatial learning and memory, N-methyl-D-aspartate receptor 2B (NR2B) mRNA and protein level in frontal cortex and hippoeampus of rat pups and to explore the mechanism of developmental neurotoxicity induced by PFOS. Methods Twenty-eight pregnant mrs were randomly divided into three groups in proportion of 3: 2: 2,including control group (C), low dose group (L) and high dose group (H) by means of randomized number table, which respectively received 0,7.2, 14.4 mg/kg PFOS feed from pregnancy day 0 to postnatal day(PND)30 by free feedings. The animal models of prenatal and postnatal non-exposure (CC), prenatal exposure (LC and HC), postnatal exposure (CL and CH), and prenatal and postnatal exposure (LL and HH) to PFOS were established by cross-fostering method. The spatial learning and memory were measured by water maze experiment,the NR2B mRNA levels in frontal cortex of rat pups was determined with semi-quantitative RT-PCR, NR2B protein express in cerebral cortex (frontal and tempera]cortex) and hippocampus (CAI, CA3, CA4 and DG regions) of rat pups was detected by immunohistochemistry. Results The escape latency of CL, CH, LL and HH groups pups in water maze experiment were (99. 83±25.77) s, (111.30±17. 82) s, (106. 40±18. 71) s, (107.70±16. 85) s, and longer as compared with CC group[(54.90±26.69)s](q value were 4.349,4.773,6.026 and 5.641, respectively,P<0. 01). The number of errors of HH group rat pups entering dead end was (22. 30±7.56) at the training day 4,and it was significantly higher than that of CC group (9. 80±4. 64) (q=5. 173,P<0. 01). The NR2B mRNA levels of frontal cortex of pups in HC group at PND1 ,and LC group, HC group and HH group at PND14 were (0. 167±0. 008), (0. 364±0. 035), (0. 341±0. 030) and (0. 328±0. 045) respectively,which were significantly lower than CC group (0.271±0.060) and (0.465±0.067) (q values were 3. 547, 3. 739, 4. 597 and 5. 006, respectively, P<0.05) . The results of immunohistochemistry indicated that NR2B protein express of the hippocampus CA1 region of pups in LC group was (0.091±0.005),and showed significant lower than CC group which was (0. 123±0.009) at PND1 (q=5. 209 ,P<0. 05). At PND14, the effect of PFOS extended to cerebral cortex and hippocampus regions. At PND28, the effects of PFOS were showed in hippocampus CA1, CA3 and temporal cortex regions. Conclusion Prenatal and postnatal exposure to PFOS should result in the spatial learning and memory damage,and the mechanism might be possibly involved in the decrease of NR2B level in cerebra lcortex and hippocampal formation regions.     

王烈教授从"热毒"论治小儿外感发热的学术思想

小儿外感发热是儿科常见的临床症状.国医大师王烈教授临证60余年,对小儿外感发热的诊断治疗有着丰富的临床经验,并创新性地提出了"无毒不发热,热因毒而起"的学术思想,提倡内治外治相结合.本文旨在阐述本理论的内容以及基于该理论所立治疗疾病之法则及拟方用药,以利于在临床上进一步推广国医大师学术思想和经验.     

趋化因子和B7分子联合应用抗肿瘤的研究进展

目的：探讨生长抑素受体（somatostatin receptor，SSTR）亚型SSTR2A、SSTR5与表皮生长因子受体（epidermal growth factor receptor，EGFR）在非小细胞肺癌（non-small cell lung cancer，NSCLC）组织中的异常表达及其临床意义。方法：用免疫组化（S-P法）检测SSTR2A、SSTR5和EGFR3种蛋白在62例NSCLC组织标本和7例癌旁肺组织标本中的表达情况，并进行预后随访。结果：在62例NSCLC组织中，SSTR2A蛋白阳性表达30例，占48．3％；SSTR5阳性表达44例，占70．9％。SSTR2A、SSTR5蛋白表达与NSCLC的TNM分期有密切相关（P〈0．05），但与NSCLC患者的年龄、性别、吸烟与否、病理类型、肿瘤大小和淋巴结转移均无明显相关（P〉0．05）。同组中，EGFR蛋白在癌旁肺组织中无一例表达，而在NSCLC组织中有35例阳性，占56．4％；EGFR蛋白的表达与NSCLC患者的年龄、性别、吸烟与否、肿瘤组织类型、肿瘤大小、TNM分期、病理分级及淋巴结转移均无显著差异（P〉0．05）。SSTR2A、SSTR5蛋白表达与EGFR蛋白的表达呈负相关关系。SSTR2A、SSTR5蛋白阳性表达者的3年生存率分别为64．5％和65．9％，阴性表达者为45．2％和22．2％，两者之间差异显著（P〈0，05）；EGFR蛋白阳性表达者的3年生存率为30．8％，阴性表达者为69．4％，两者之间差异显著（P〈0．05）；结论：SSTR2A、SSTR5和EGFR的表达能提示非小细胞肺癌的生物学行为，三者的联合检测对非小细胞肺癌淋巴结转移、病理分期及预后的评估有临床意义。     

慢病毒载体在肿瘤免疫治疗中的应用

慢病毒载体感染的宿主广,不仅可以高效感染分裂期细胞,而且可以感染非分裂期细胞,可作为肿瘤免疫治疗的工具.与其他逆转录病毒载体相比,慢病毒载体具有许多优点,携带的外源基因在宿主中表达时间长、毒性低、可携带的外源基因片段大、不易诱发宿主免疫反应.本文对携带肿瘤相关抗原基因的慢病毒载体疫苗以及DC疫苗在肿瘤免疫治疗中的应用及安全性问题进行了综述,讨论慢病毒载体作为肿瘤免疫治疗的临床应用前景.     

S100钙结合蛋白A4在大鼠动脉粥样硬化中的作用

目的探索钙离子拮抗剂(CCB)和干预S100钙结合蛋白A4(S100A4)表达对SD大鼠下肢动脉硬化性闭塞症(ASO)动物模型发病进程的影响。方法将56只健康SD大鼠采用随机数字表分组法等分为8组, 每组7只。A组为对照组, B组为假手术组, C、D、E、F、G、H组则为通过采用手术联合高脂饮食的方式构建大鼠经典ASO模型后, 鼠尾静脉注射CCB、Lv-S100A4、S100A4小干扰RNA(siRNA)。通过茜素红染色评估股动脉中Ca2+浓度的改变, 苏木精-伊红(HE)染色评估股动脉内膜、中膜增生情况, 免疫组织化学检测股动脉S100A4的蛋白表达。计量资料两组间比较用独立样本t检验;多组间比较用单因素方差分析。计数资料采用χ2检验。等级资料采用秩和检验。结果 16周后成功构建大鼠ASO模型。F组中S100A4的表达显著低于E组(0.24±0.03比0.42±0.05, t=-6.774, P<0.01)且F组股动脉内膜及中膜厚度明显低于E组[(82.98±4.76) μm比(101.26±3.18) μm, t=-8.451, P<0.01]。H组中S100A4的表达...     

国医大师王烈教授从肾脑论治儿童抽动障碍经验

抽动障碍是一种以不随意的、突然发生的、快速的、反复出现的、无明显目的的、非节律性的一个或多个部位运动抽动和（或）发声抽动为主要特征的综合征，王烈教授认为抽动障碍的病机关键在于脑、肾不足，发病与五脏六腑功能失调相关，治疗上应注重安脑滋肾，治病求本，同时强调家庭精神调护，临床上取得良好的疗效。