Reversal of multi-drug resistance by vector-based-ShRNA-Mdr1 <Emphasis Type="Italic">In Vitro</Emphasis> and <Emphasis Type="Italic">In Vivo</Emphasis>

In order to investigate the effects of vector-based hairpin small interference RNA （shRNA） on the reversal of multi-drug resistance （mdr） of A2780/Taxol cells, a novel vector pEGFP-HI/mdrl containing mdrl-shRNA targeting at position 2943-2963 of mdrl was designed and synthesized. Subsequently, A2780/Taxol cells were transfected with pEGFP-H1/rndrl, and the expression ofmdrl mRNA and P-gp was detected by using RT-PCR and Western blot respectively. MTT was used to measure the 50% inhibition concentration （IC50） of Taxol to A2780/Taxol cells. The results showed that at the 24th and 48th h after transfection, the expression of mdrl mRNA was decreased to （52.1±1.0）% and （0.01±1.7）%, and that of P-gp decreased to （88.3±2.1）% and 0%, respectively. At the 48th h after transfection, the relative reversal rate of A2780/Taxol cells to Taxol was 69.54%. In vivo, the nude mice xenografts were injected with pEGFP-H1/mdrl, and then administrated Taxol. The tumor volume in pEGFP-H1/mdrl-transfected group was significantly reduced as compared with that in blank control group or pEGFP-Hl-transfected group （807.20±103.16 vs 1563.78±210.54 or 1480.78±241.24 mm^3, both P〈0.01）. These results suggested that transfection of pEGFP-HI/mdrl could efficiently down-regulate the expression of mdrl mRNA and P-gp in A2780/Taxol cells, and effectively restore the sensitivity of A2780/Taxol ceils to Taxol both in vitro and in vivo.     

The role of SDF-1/CXCR4 axis in ovarian cancer metastasis

This study was aimed to explore the role of stromal-derived factor 1 （SDF-1）/CXC chemokine receptor 4 （CXCR4） axis in mediating the metastasis of ovarian cancer cells through activation of extracellular signal-regulated kinase-1/2 （ERK-1/2） signaling pathway. A highly metastatic ovarian cancer cell line, SKOV3, was used in the study. Intracellular calcium mobilization was detected by using laser scanning confocal fluorescence microscopy. Western blotting was used to detect the phosphorylation of ERK1/2 in SDF-1α-treated SKOV3 cells. Adhesion capability and matrix metalloproteinase （MMP） activity of ovarian cancer cells after exposure to SDF-1 a were measured by adhesion assay and gelatin zymography. The results showed that SDF-1α induced rapid intracellular calcium mobilization in SKOV3 cells, as well as the phosphorylation of ERK-1/2. The adhesion of ovarian cancer cells to fibronectin and collagen Ⅳ was increased after SDF-1α treatment. An inhibitor of ERK-1/2 signaling, PD98059, could antagonize such effects of SDF-1α. SDF-1α could also increase the secretion of active MMP-2 and MMP-9. It was concluded that the SDF-1/CXCR4 axis played a critical role in the metastasis of human ovarian cancer by increasing the adhesion capability of cancer cells and the activity of MMP-2 and MMP-9 via ERK1/2 signaling pathway.     

异丙酚对家兔主动脉阻断所致脊髓损害的保护作用

目的 研究异丙酚对家兔主动脉阻断后脊髓损害的保护作用。方法 家兔１８只，随机分为假手术组，缺血组和异丙酚组。肾下阻断腹主动脉４０分钟后松开。Ｃ组于阻断前静脉注射异丙酚５ｍｇ．ｋｇ＾－１，继以２０．ｍｇ．ｋｇ＾－１．ｈ＾－１微量泵静脉输注至松开前，余２组则以同样方法静注等容量生理盐水作对照。     

经腹超声对晚期妊娠前置胎盘的诊断和鉴别诊断

目的:探讨经腹超声对晚期妊娠前置胎盘诊断和鉴别诊断的价值。方法:回顾分析50例晚期妊娠孕妇的超声诊断前置胎盘图像资料,与产后诊断进行比较。结果:50例腹部超声检查,符合产后临床诊断的45例,诊断准确率为90%,漏诊后壁胎盘2例,侧壁胎盘1例,误诊2例。结论:经腹超声能提高前置胎盘的准确率,但需要鉴别诊断及正确的操作方法。     

雌二醇屈螺酮片治疗绝经后妇女绝经相关症状的多中心临床研究

Objective To study the efficacy and safety of estradiol and drospirenone tablets (Angeliq)in treatment of menopausal symptoms among postmenopausal Chinese healthy women.Methods Total 244 postmenopausal Chinese healthy women who had moderate to severe hot flushes were randomly assigned for 16 weeks in this randomized multi-center double-blind placebo-controlled study.During the trial.the follow-up visits were conducted at week 4,8,12,16 of treatment and 2 weeks after treatment respectively.Height,weight,vital signs,hot flushes,other relevant menopausal symptoms and vaginal bleeding were observed in each follow-up visit,while the clinical global impression scale Was assessed at 16 weeks as well.Results It showed that hot flushes were reduced significantly more in observation group than that in placebo group ( P＜0.01 ), although both treatments were effective. The absolute values of mean severity index of total hot flushes decreased by - 0. 6± 0. 5 in observation group and - 0. 4 ± 0. 4 in placebo group from baseline respectively, which reached significant difference ( P ＜ 0. 05 ). However, the absolute values of mean severity index of moderate to severe hot flushes decreased by - 0. 6± 0. 8 in observation group and -0. 3± 0.6 in placebo group from baseline respectively, which had no significant difference (P ＞ 0. 05 ).After 16 weeks treatment, it also showed that estradiol and drospirenone had significant better efficacy than placebo on moderate to severe sweating, vaginal dryness and clinical global impression scale (P ＜0. 01 ).During the trial, blood pressure in observation group was stable. The rate of vaginal bleeding in observation group was higher than that in the placebo group, especially during the week 4 to week 8 when 48. 9% (87/178) in observation group and 10. 7% (6/56) in placebo group of patients bled. Although the cumulative amenorrhea rate of observation group was lower than that of placebo group in each cycle (28 days), it increased gradually along with duration of the treatment. The commonest adverse event in observation group was breast tenderness which accounted for 12.0% (22/183 ). The level of serum potassium was in the normal range in observation group mostly. Meanwhile, the other adverse events rate was low. Serious adverse events reported in this trial were assessed as not study drug related or as unlikely study drug related. Conclusion Estradiol and drospirenone tablets which could effectively alleviate menopausal symptoms in postmeuopausal Chinese healthy women is a novel hormone replacement therapy regimen with high safety and efficacy.     

夹脊排针法合督灸治疗腰椎间盘突出症的疗效观察

目的:观察夹脊排针法合督灸改善腰椎间盘突出症患者疼痛与功能的有效性和安全性。方法:随机将符合标准的80例腰椎间盘突出症患者分成对照组和观察组,每组各40例,对照组给予夹脊排针法治疗,观察组在对照组的基础上加用督灸;两组共治疗2周。比较两组治疗前后视觉模拟评分法(VAS)和日本下腰痛JOA评分,评价两组患者疼痛与功能改善情况。结果:治疗结束后,观察组总有效率为87.5%,高于对照组的77.5%,两者的临床总有效率有显著差异(P<0.01);两组患者的VAS评分都较前有所降低(P<0.01),JOA评分均比治疗前明显升高(P<0.01),并且观察组的评分改善程度远优于对照组(P<0.01)。结论:夹脊排针法合督灸有利于改善腰椎间盘突出症患者的疼痛及腰椎功能。     

高强度聚焦超声对早孕大鼠蜕膜组织中白血病抑制因子、基质金属蛋白酶-9表达的影响

目的探讨高强度聚焦超声（HIFU）终止早孕的机制.方法妊娠第8天 SD大鼠48只,每组8只,用780KHz连续超声波5组超声声强开腹辐照子宫胚胎着床点各90秒,分别于妊娠第8天和15天取材,设妊娠第8天大鼠胚胎为对照组.应用免疫组化法检测早孕大鼠胚胎着床点蜕膜组织中白血病抑制因子（LIF）和基质金属蛋白酶-9（MMP-9）的表达.结果各辐照组LIF和MMP-9早孕蜕膜组织中表达明显小于对照组 （p〈0.01）,并随声强的增强而表达逐渐减弱.结论 LIF、MMP-9的变化可能是使HIFU终止大鼠早孕的关键.     

2198例液基细胞学宫颈癌筛查的临床分析

目的:评价Auto Cyte PREP液基细胞学对宫颈癌筛查的临床价值。方法:取2 198例受检者的宫颈脱落细胞作液基薄片细胞学检查,细胞学诊断采用TBS分级系统,阳性诊断包括鳞状上皮内低度病变(LSIL)及以上病变。对于意义不明的不典型鳞状细胞(ASCUS)及以上的受检者行阴道镜活检,并将两种结果作对照。结果:Auto Cyte PREP液基细胞学标本满意度高,检测的敏感性、特异性和准确性分别达到81.6%、91.4%和87.7%,对鳞状细胞癌(SCC)、鳞状上皮内高度病变(HSIL)、鳞状上皮内低度病变(LSIL)的分级敏感性分别为100.0%(10/10)、85.9%(61/71)、77.9%(106/136)。细胞学与组织学级别基本达到一致。结论:液基细胞学应用于宫颈癌筛查能明显提高标本质量和宫颈异常细胞诊断的准确性,尤其是对HSIL。     

妊娠期维生素和微量元素的补充

孕妇营养状况直接影响胎儿的生长发育,近年来,维生素、微量元素对胎儿生长发育的影响逐渐受到重视。因此,加强围生期宣传教育、提高孕妇的自我保护意识、及时给予合理的膳食指导和建议是围生期保健中一个必不可少的环节。1妊娠期维生素的补充维生素是许多酶的辅酶或辅酶的组成分子,是维持和调节机体正常代谢的重要物质,大部分不能在人体内合成或合成量不足,必须从食物中摄取。维生素缺乏会造成孕妇营养不良,胎儿生长受限、发育不良,严重者会导致胎儿畸形和胎死宫内。维生素可分为脂溶性(A、D、E、K)和水溶性(B、C)两大类。由于胎盘的屏障…