Study on Tumor Angiogenesis in Epithelial Ovarian Carcinoma

The tumor angiogenesis has been recognized asthe neovascularization induced by tumor cells, and finally the formation of capillary network to supply thetumor mass. Recently, it has been shown that thetumor angiogenesis played a key role in the occurrence, development, metastasis and prognosis of tumor. Several studies about epithelial ovarian tumorangiogenesis have been reported abroad, but their results are in controversy['J.' The purpose of this studywas to investigate tumor angiogenesis in …     

雌二醇屈螺酮片治疗绝经后妇女绝经相关症状的多中心临床研究

Objective To study the efficacy and safety of estradiol and drospirenone tablets (Angeliq)in treatment of menopausal symptoms among postmenopausal Chinese healthy women.Methods Total 244 postmenopausal Chinese healthy women who had moderate to severe hot flushes were randomly assigned for 16 weeks in this randomized multi-center double-blind placebo-controlled study.During the trial.the follow-up visits were conducted at week 4,8,12,16 of treatment and 2 weeks after treatment respectively.Height,weight,vital signs,hot flushes,other relevant menopausal symptoms and vaginal bleeding were observed in each follow-up visit,while the clinical global impression scale Was assessed at 16 weeks as well.Results It showed that hot flushes were reduced significantly more in observation group than that in placebo group ( P＜0.01 ), although both treatments were effective. The absolute values of mean severity index of total hot flushes decreased by - 0. 6± 0. 5 in observation group and - 0. 4 ± 0. 4 in placebo group from baseline respectively, which reached significant difference ( P ＜ 0. 05 ). However, the absolute values of mean severity index of moderate to severe hot flushes decreased by - 0. 6± 0. 8 in observation group and -0. 3± 0.6 in placebo group from baseline respectively, which had no significant difference (P ＞ 0. 05 ).After 16 weeks treatment, it also showed that estradiol and drospirenone had significant better efficacy than placebo on moderate to severe sweating, vaginal dryness and clinical global impression scale (P ＜0. 01 ).During the trial, blood pressure in observation group was stable. The rate of vaginal bleeding in observation group was higher than that in the placebo group, especially during the week 4 to week 8 when 48. 9% (87/178) in observation group and 10. 7% (6/56) in placebo group of patients bled. Although the cumulative amenorrhea rate of observation group was lower than that of placebo group in each cycle (28 days), it increased gradually along with duration of the treatment. The commonest adverse event in observation group was breast tenderness which accounted for 12.0% (22/183 ). The level of serum potassium was in the normal range in observation group mostly. Meanwhile, the other adverse events rate was low. Serious adverse events reported in this trial were assessed as not study drug related or as unlikely study drug related. Conclusion Estradiol and drospirenone tablets which could effectively alleviate menopausal symptoms in postmeuopausal Chinese healthy women is a novel hormone replacement therapy regimen with high safety and efficacy.     

子宫颈癌中叉头框C2蛋白的表达及其意义

宫颈癌是女性常见的恶性肿瘤,其发病率及病死率在我国呈上升趋势.宫颈癌转移是一个复杂的、连续的、多阶段的过程.肿瘤血管生成理论认为,肿瘤的生长、转移依赖于血管生成,而以直接蔓延为主要转移途径的宫颈癌,其发展和转移更有赖于新生血管的形成.     

巨块型宫颈癌ⅠB2～ⅡB期68例术前全身新辅助化疗的近期疗效观察

目的 探讨术前新辅助化疗对巨块型宫颈癌的近期疗效.方法 选择2001年12月至2004年12月华中科技大学同济医学院附属协和医院初治的经病理确诊的68例ⅠB2～ⅡB期巨块型宫颈癌,采用PVB方案化疗2个疗程后手术,观察近期的疗效.结果 临床近期有效率达75%,手术切除率达100%.术前新辅助化疗可提高手术切除率和症状改善率,化疗后肿瘤直径较化疗前有不同程度的缩小,差异有显著意义（P＜0.05）.所有手术患者均能顺利切除病灶并达到切缘距离病灶1cm以上.结论 术前新辅助化疗可提高宫颈癌的近期疗效,成为治疗该病的一种新手段,具有重要的临床意义.     

紫草联合MTX治疗宫外孕的疗效观察

宫外孕是妇科常见的一种病症。近20年来，它的发生率增加了2～3倍，占妊娠总数的近2％。如不及时予以治疗，会引起腹腔内大出血、失血性休克等，甚至危及生命安全。在药物保守治疗的基础上，大多医院都以MTX治疗为主。     

76例宫颈癌患者的临床特征及预后影响因素分析

近年来,宫颈癌的发病呈年轻化趋势.现对76例宫颈癌患者的临床特征进行分析,并探讨预后影响因素.     

正常妊娠妇女及妊娠期高血压疾病患者血浆D-二聚体改变的前瞻性研究

目的:研究正常妊娠以及妊娠期高血压疾病母体血浆D-二聚体(D-dimer,D-D)水平随妊娠发展的变化趋势.方法:应用前瞻性研究方法,对180例妊娠前血压正常的孕妇,检测母体血浆D-D在妊娠10～14、20～24、30～34周的水平,观察D-D在孕妇孕期变化情况并随访妊娠结局;选取50例血压正常未孕妇女作为对照.结果:①180例进入本研究妊娠妇女,12例发生妊娠期高血压、10例子痫前期(4例重度、6例轻度)、158例孕妇妊娠结局正常;②正常妊娠后,母体血浆D-D浓度在妊娠10～14周高于正常未孕妇女,差异有统计学意义(P<0.01),妊娠10～14、20～24、30～34周、随孕周增加,母体血浆D-D浓度逐渐上升,差异均有统计学意义(均P<0.01);③子痫前期、妊娠期高血压、正常妊娠妇女母体血浆D-D浓度在妊娠10～14、20～24、30～34周,同期比较差异均无统计学意义(均P>0.05).结论:正常妊娠妇女与妊娠期高血压疾病患者母体血浆D-D随着妊娠进展浓度逐渐增加,但在本研究中2者间差异无统计学意义,故以其作为疾病早期预测指标宜谨慎.     

米非司酮对早孕胎盘绒毛诱导型一氧化氮合酶和细胞凋亡的影响

目的了解米非司酮对早孕绒毛诱导型一氧化氮合酶（iNOS）及滋养层细胞凋亡的影响,进一步探讨米非司酢早孕药物流产机理。方法选择40例早孕药物流产患者（实验组）,采用原位杂交及免疫组化方法检测绒毛中iNOS的表达,细胞凋亡采用3＇-OH末端缺口标记法（TUNEL）显示绒毛滋养细胞中原位凋亡细胞的表达。利用计算机图象分析系纺（CMIAS）,iNOS表达指标以数密度（N／S）及阳性单位（Pu）计算,细胞凋亡以每个统计场凋亡细胞个数（N）、凋亡细胞平均噘光度（OD）、凋亡细胞面密度（凋亡细胞面积／统计场面积,DS／TS）表示,并与40例正常人工流产的绒毛和蜕膜组织（对照组：比较。结果实验组绒毛组织iNOS原位杂交结果显示N／S、Pu分别为0．12±0．010、15．3±2．6,对照组分别为0．02±0．003、3．1±0．5,两组有极显著性差异（P〈0．01）;免疫组化结果显示实验组和对照组N／s、Pu分别为0．09±0．01、10．24±1．55及0．016±0．002、1．26±0．33,差异有显著性。实验组绒毛滋养细胞凋亡指数显著增高,各指标两组比较差异显著。结论米非司酮早孕药物流产与绒毛组织iNOS活性及滋养细胞凋亡有关。     

细胞凋亡及Fas、Bcl-2 mRNA在早孕药物流产绒毛组织中的表达

目的 了解早孕药物流产局部绒毛组织中细胞凋亡及Fas、Bcl-2mRNA表达,进一步探讨米非司酮早孕药物流产机制.方法 选择40例早孕药物流产患者(实验组),采用3'-OH末端缺口标记法(TUNEL)显示绒毛滋养细胞中原位凋亡细胞的表达,原位杂交法显示绒毛滋养细胞中Fas、Bcl-2mRNA表达,利用计算机图象分析系统(CMIAS)分析每个统计场凋亡细胞个数(n)、凋亡细胞平均吸光度(OD)、凋亡细胞面密度(凋亡细胞面积/统计场面积,DS/TS)、Fas、Bcl-2mRNA原位杂交阳性表达细胞的平均光密度(D)及每个统汁场阳性细胞数(N/S).并与40例正常人工流产比较(对照组).结果 实验组绒毛滋养细胞中凋亡细胞表达显著增高,各指标两组比较差异显著.FasmRNA表达实验组高于对照组,Bcl-2mRNA表达实验组低于对照组,两组比较均有极显著性差异(P<0.01).结论 Fas、Bcl-2mRNA介导的细胞凋亡可能是米非司酮早孕药物流产的重要机理之一.     

Effects of Livin gene RNA interference on apoptosis of cervical cancer Hela cells and enhanced sensitivity to cisplatin

The recombinant plasmids pGenesil-1-BIRC71 and pGenesil-1-BIRC72 were transfected into Hela cells and cisplatin was added with different concentrations in order to study the inhibitory effects of Livin gene, increase the apoptosis induced by cisplatin, and detect the expression ofBcl-2, Bax, caspase-3, and survivin genes. The pGenesil-1-BIRC71 and pGenesil-1-BIRC72 were transfected into Hela cells, and the expression levels of Livin, Bcl-2, Bax, caspase-3, and survivin genes were detected by using fluorescence quantitative real-time PCR. Then cisplatin at different concentrations （3.0, 6.0 and 9.9 μg/mL） was added into the transfected Hela cells, and 24, and 48 h later, the apoptosis rate was measured by flow cytometry. After transfection of pGenesil-l-BIRC71 and pGenesil-1-BIRC72 into Hela cells, the expression level of Livin gene was obviously reduced, and the apoptosis rate was significantly increased in transfection group as compared with control group （P〈0.05）. Cisplatin could increase the apoptosis rate in a dose- and time-dependent manner. After cisplatin was added, the expression levels of Bcl-2 mRNA were reduced, and those of Bax, caspase-3, and survivin mRNA were increased in transfection group as compared with those in control group （P〈0.05）. It was concluded that shRNA expression vector targeting Livin gene could inhibit the expression of Livin gene in Hela cells and enhance the apoptosis induced by cisplatin, which was related to the decreased expression of Bcl-2 and activation of Bax and caspase-3. Survivin might play an important role as an antagonist in the process of apoptosis induction.