椎弓根螺钉内固定治疗多节段腰椎骨折的临床

目的 探讨应用TSRH-3D椎弓根螺钉系统复位并椎弓根内固定治疗多节段腰椎骨折的治疗效果及远期疗效.方法 本组18例多节段腰椎骨折患者应用TSRH-3D椎弓根螺钉系统复位并椎弓根内固定治疗多节段腰椎骨折,均于伤后4 h～2 d内手术,所有患者均得到随访,随访时间15～32个月,平均23个月.均于术前、术后、末次随访时拍摄脊柱正侧位X片和CT片.结果 (1)脊柱活动改善率为67%;腰背痛改善率为94%;下肢疼痛改善率为78%.(2)与术前相比,伤椎前缘高度平均矫正59.2%,平均丢失5.2%(P<0.01);Cobb角平均矫正19.5°,平均丢失5.4°(P<0.01)°结论 采用TSRH-3D椎弓根螺钉内固定系统治疗多节段腰椎骨折对损伤节段可有满意的复位及内固定效果,有利于恢复脊柱伤椎高度、生理弧度和神经功能以及消除腰腿痛,有较好的远期疗效.     

双极人工股骨头置换术治疗高龄患者股骨颈骨折

目的探讨双极人工股骨头置换术治疗高龄股骨颈骨折的临床疗效。方法自1998年5月～2004年8月．采用双极人工股骨头置换术治疗28例老年股骨颈骨折患者。结果28例患者平均随访时间8个月（6个月～4年）。根据Harris评分方法判断疗效：优10例,良12例,可5例,差1例。结论双极人工股骨头置换术是治疗高龄有移位的股骨颈骨折的一种可供选择的方法。     

Molecular Characterization of Duck Hepatitis B Virus Isolated from Hubei Brown Ducks

The objective of this study was to characterize the genome structure of duck hepatitis B virus (DHBV) isolated from Hubei brown ducks. The natural carrier rate of DHBV in adult ducks from Hubei area was investigated and the DHBV DNA-positive serum screened out. The complete genome of a DHBV strain was amplified by polymerase chain reaction (PCR) and cloned into T vector and sequenced. The results showed that the carrier rate of DHBV in Hubei brown ducks was 10 %. This strain (GenBank accession number DQ276978) had a genome of 3024 nucleotides with three overlapping open reading frames encoding the surface, core and polymerase proteins respectively. Comparison of the strain with 17 DHBV strains registered in GenBank revealed a homology from 89.3 % to 93.5 % at the nucleotide level. The sequences of the structural and functional domains of these proteins were highly conserved. The strain was found to share more signature amino acids in the polymerase genes with the "Chinese" DHBV strains than those of the "Western" country strains. This finding was also corroborated by a phylogenetic tree analysis. Therefore, the DQ276978 might belong to a subtype of the Chinese DHBV strains.     

幽门螺杆菌对甲硝唑耐药性及多重相关基因DNA和氨基酸序列分析

目的 了解幽门螺杆菌(Hrlicobacter pylori，Hp)对甲硝唑的耐药状况，研究相关的多重基因突变，为深入揭示Hp耐药的分子机制提供相关资料。方法 分离培养54株Hp，以琼脂稀释法检测Hp的甲硝唑最低抑菌浓度(deterimination of minimal inhibitory concentration，MIC)，用PCR方法扩增甲硝唑耐药相关基因(rdxA、frxA、fdxB)，经分子克隆后DNA测序，分析敏感株和耐药株耐药基因的序列及其与耐药性的关系。结果 武汉地区人群Hp对甲硝唑的耐药率为67％；甲硝唑耐药基因序列分析表明，耐药临床株的rdxA和(或)frxA基因存在插入、缺失和点突变。结论 Hp对甲硝唑耐药不仅与rdxA有关，同时存在frxA、fdxB基因突变。frxA、fdxB基因的突变可能与rdxA在Hp耐甲硝唑中有协同作用。     

Whole CanA Gene Amplification of Helicobacter pylori and Its Fingerprinting by Restriction Fragment Length Polymorphism

To set up a method of amplification for the whole CagA gene of Helicobacter pylori and its fingerprinting by restriction fragment length polymorphism(RFLP),nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind Ⅲ were used to generate the RFLP fingerprinting.Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained.It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.     

交叉钢针张力带治疗尺骨鹰嘴骨折37例

交叉钢针张力带治疗尺骨鹰嘴骨折３７例李爱武王勇王兆阳张正茂＊（益都中心医院山东青州２６２５００＊淄博市第三人民医院）自１９９０年至１９９５年，我们应用交叉钢针加张力带治疗尺骨鹰嘴骨折３７例，效果良好，现报告如下。１临床资料本组病例中，男２４例，女１３...     

内镜椎间盘切除联合激素治疗L5S1椎间盘脱出症

[目的]探讨经皮脊柱内镜下椎间盘切除术(percutenous endoscopic lumbar discectomy,PELD)联合激素(glucocorti-coid,GC)治疗L5S1椎间盘脱出症的疗效.[方法]回顾性分析本院2015年6月-2019年1月PELD治疗的单纯L5S1椎间盘脱出症116例患者的临床...     

湖北地区幽门螺杆菌VacA基因分型及疾病相关性分析

目的了解湖北地区幽门螺杆菌（Hp）空泡毒素基因（vacA）不同噩型及其与慢性胃炎（CG）和慢性消化性溃疡（CPU）之间的相关性。方法采用共享引物PCR技术和特异的PCR分型引物，对74例CG或CPU患者胃黏膜活检组织Hp分离株进行分型，并分析不同亚型与这两种疾病的相关性。结果湖北地区的HpvacA基因主要以S1a—m2型为主，vacA的优势亚型分布与上海和广州相似．除了s2型以外，sla—ml、S1b—m1、s1b—m2、s1c—m1和S1c—m2均有分布；另有8株不能被完全分型。结论湖北地区Hp vacA基因的不同亚型与CG和CPU之间不存在相关性，CG和CPU的引发因素有待进一步研究。     

伤椎置钉GSS系统治疗胸腰段脊柱骨折52例疗效观察

目的探讨GSS加伤椎置钉系统治疗胸腰段脊柱骨折的可行性和手术疗效。方法回顾性分析2007年10月～2011年2月采用短节段GSS加伤椎置钉系统治疗胸腰段脊柱骨折52例患者临床资料及术后Cobb角、骨折复位及症状改善情况。结果 52例手术均成功,术后随访12～29个月、平均19个月。Cobb角术前(29.6±9.1)°,术后恢复到(5.1±6.2)°,两者比较P<0.05;椎体前缘高度由术前为正常的(40.9±17.7)%,术后恢复为正常的(94.9±10.2)%,两者比较P<0.05。术前脊髓神经损伤按Frankel分级A级6例,B级5例,C级6例,D级19例,E级16例。术后除3例A级和1例C级外,均有1～3级的恢复。术后无感染,无内固定松动、断裂。结论同时经伤椎置钉的短节段GSS系统复位固定治疗胸腰段脊柱骨折可获得良好的复位,增加术后稳定性,是一种切实可行而有效的方法。     

转染CD40配体的卵巢癌细胞株OVHM抗卵巢癌肝转移机制的研究

Objective To examine the in vivo anti-metastatic effect of enhanced expression of CD40L cDNA in murinc ovarian cancer OVUM cells (CD4OL-OVHM) injected into the spleen on liver metastasis in mice. Methods OVUM cells were inoculated into the spleen of 6 ～ 8 week-old female B6C3F1 (C57BL/6N × C3H/He) mice. The established liver metastasis was identified by histopathology (HE staining). OVUM cells, DNA-pMKITneo-OVHM cells or CD40L-OVHM cells were inoculated into the spleen of female B6C3F1 mice and the expressions of CD11c in splenic cells were detected by flow cytometry. The specific cytotoxicity of splenic cells was detected by MTT assay, and the serum cytokines of IFN-γ, TNF-α, IL-12, IL4 and IL-10 of the mice were measured by enzyme linked immunoabsorbent assay. The liver metastases and the survival time of the mice were also recorded. Results The mouse models with liver metastasis by injecting tumor cells into the spleen of mice were established. The expression of CD11c and the specific killing rote in CD40L-OVHM cells group was significantly higher than that in the OVHM cells group and DNA-pMKITneo-OVHM cells group. The expressions of IFN-γ, TNF-α and IL-12 in the CD40L-OVHM cells group were much more increased than OVHM cells group and DNA-pMKITneo-OVHM cells group, but the expressions of IL-4 and IL-10 in the CD40L-OVHM cells group were decreased significantly (P < 0.05). The average weights of livers and spleens of mice in CD40L-OVHM cells group were significantly lower than those of DNA-pMKITneo-OVHM cells group and OVHM cells group. The survival time of mice in CD40L-OVHM cells group was also significantly longer than that in the OVHM cells group and DNA-pMKITneo-OVHM cells group (P < 0.05). Conclusion The data directly demonstrate that the expression of CD40L in ovarian cancer cells (CD40L-OVHM) can enhance the proliferation and differentiation of dendritic cells in the spleen and induce specific cytotoxic effect of T cells in the spleen, and may regulate the immune function of peripheral blood cells and the immune balance between Thl cells and Th2 cells, which maybe the possible mechanism induced by CD40L in mice inhibiting the development of liver metastasis.