连续血液滤过对严重脓毒症患者免疫抑制状态的影响

目的：探讨连续性静-静脉血液滤过（continuous venovenous hemofiltration，CVVH）对严重脓毒症患者血流动力学和外周血辅助性T细胞亚群Th1/Th2平衡的影响。方法：前瞻性观察应用CVVH治疗18例严重脓毒症患者，于CVVH治疗前和治疗后4、8h抽取静脉血应用流式细胞仪检测外周血辅助性T细胞亚群Th1／Th2比值变化，同时观察生化指标、血流动力学变化。结果：18例患者CVVH治疗后血清BUN、Scr较治疗前均呈下降趋势（P〈0．01），MAP显著上升（P〈0．01）。而多巴胺、去甲肾上腺素用量显著降低护〈0．01），Th1／Th2比值呈上升趋势，差异具有统计学意义（P〈0．05或P〈0．01）。结论：CVVH能改善严重脓毒症患者血流动力学及免疫麻痹状态。     

急性胆管损伤的治疗(附10例报告)

肝外胆管损伤，如不及时处理或处理不当可发生胆管狭窄，引起胆管反复感染造成肝功能损害。1985～2002年我们对外伤及术中损伤胆管10例及时作了处理，现将诊治体会报告如下。     

MEC-509监护仪电源故障检修二例

1故障现象一按下电源开关键“POWER”,机器不能启动工作。故障分析与检修:该机电源的控制原理:当电源开关键“POWER”按下时,U17(7474)5脚经R65接地为低电平,6脚为高电平,U18(7474)输出端输出高电平,Q3导通,U24(78S40)接地工作,有12V电压输出。同时Q13、Q14导通,U19(TL497)得到     

双小腿离断成功再植一例

报道 :2 0 0 1年 7月 14日我院收治一例双小腿下段完全离断 ,再植成活术后 ,随访 3 0个月 ,现患者可独立行走及上下楼 ,双足功能恢复满意。体会 :分组手术、骨折采用简便的固定以缩短再植时间 ;术后注意预防再植后毒素吸收所致肾损伤。双小腿离断成功再植一例@王兵$昆明医学院第一附属医院骨科 @赵宏斌$昆明医学院第一附属医院骨科 @赵学凌$昆明医学院第一附属医院骨科 @李林芝$昆明医学院第一附属医院骨科 @张春强$昆明医学院第一附属医院骨科 @王宏邦$昆明医学院第一附属医院骨科…     

自拟“表清汤”治疗肺炎113例

我们自1992年,用自拟“表清汤”治疗肺炎,取得满意疗效,现将资料完整的113例总结如下:1 一般资料 113例均是我院门诊病人,其中男88例,女25例;年龄最大76岁,最小8岁,平均年龄37岁;病程最长9天,最短1天,平均病程3.5天。所有病人均作胸部X线透视或拍片,提示肺纹理增强或有片絮状阴影,血化验检查白细胞数增高,或中性粒细胞比例增大,胸部听诊有呼吸音改变,或有肺部罗音,临     

显微手术治疗肘管综合征的优点

显微手术治疗肘管综合征的优点王兵１９８２～１９９４年，我们收治３３例肘管综合征（亦称迟发性尺神经炎Ｃｕｂｉｔａｌｔｕｎｎｅｌｓｙｎｄｒｏｍｅ，ｔａｒｄｙｕｌｎａｒｎｅｕｒｉｔｉｓ），分别采用手术松解，手术松解皮下前置术、显微手术松解移位术等三种术式。...     

山东滕州市农村小学生弱视斜视及立体视锐度调查

Objective To investigate the visual acuity, stereoacuity and the prevalence of amblyopia and strabismus ofprimazy school children in rural areas in Tengzhou, Shandong as well as to evaluate the effi-ciency of three methods in screening amblyopia. Methods In this population-based study, children aged 5 to 14 years old in primary school were randomly selected by a cluster sampling in which school shift was the sam- piing unit. Titmus stereo-test, visual acuity (VA), cover-test and ocular motility evaluation were examined for each child. Retinoscopy was performed for children with VA < 1.0 in either eye. Results ( 1 ) Of 2668 effec-tive examinatious,369 eases (13.83%) were poor eyesight. (2) The prevalence ofamblyopia and strabismus were 1.50% and 1.72% respectively. Out of 40 amblyopia children, 39 were screened at the first time. (3) The stereoacuity in normal children was 40″, 31 (77.5%) children with amblyopia showed abnormal stereoacuity,while 28.3% for strabismus. (4) Specificity and crude agreement of combined Titmus with VA were 99.47%and 99.06% ,which were highest in the three methods. The second was Titmus stereo-test with 97.98% and 97.59%, VA was the lowest. Conelusion The most important cause of poor eyesight in primary school chil-dren is myopia. Amblyopia and strabismus might have significantly effects on stereoacuity. The most efficient method of screening amblyopia was stereo-test combined with visual acuity.     

体外转染PTEN基因对人结肠癌细胞生长的影响

目的探讨野生型PTEN基因表达对结肠癌细胞体外生长的影响。方法应用脂质体转导技术,将含野生型PTEN基因重组真核表达质粒pBp-PTEN转染Lovo细胞并稳定表达。Western blotting鉴定后,观察Lovo细胞转染前后生长、细胞周期、凋亡的改变。结果转染后细胞生长曲线提示转染组曲线平缓,无明显对数生长期,生长速度较对照组明显降低(P<0.01);细胞周期测定显示转染组细胞G1期比例上升,提示PTEN可能对G1/S期转换点起阻滞作用;转染组细胞凋亡比率增高(P<0.01),尤其是早期凋亡。结论外源性PTEN基因的转染可以抑制结肠癌细胞的体外生长。