兔动静脉短路环法诱导组织工程骨支架材料血管生成的初步研究

Objective To compare the effects of 2 vascular carriers, arteriovenous loop and arteri-ovenous bundle, on inducing angiogenesis in coral scaffold of vascularized tissue-engineered bone in animal models.Methods Thirty-six adult male New Zealand rabbits were randomized into 2 even groups.In group A, an arteriovenous loop (AVL) was formed by microsurgical anastomosis at the proximal ends between the femoral poptiteal artery and vein, and placed in the circular side groove of the coral block (6 mm × 8 mm × 10 mm) .In group B, flow-through vessels bundles of both femoral artery and vein were placed in the side grooves of the coral block.All the implants in 2 groups were wrapped by a micro-porous expand-ed-polytetrafluoroethylene (ePTFE) membrane, and fixed subcutaneously by suturing.Evaluation methods included gross morphological observations, histological examinations, India ink perfusion and vascular casting after 2, 4, 6 weeks.The density of blood vessels was analyzed by the statistical software SPSS 10.0.Results All the corals were encased by newly formed fibrovascular tissues in 2 groups.Ink-stained vessels distributed the surfaces and side grooves, and invaded the interspaces of corals.The degree of vascularization increased over the course of experiment.Blood vessel density demonstrated a significant continuous increase between 2 and 6 weeks after implantation in group A.The mean value of blood vessel density in group A (2 weeks 276.60±4.67, 4 weeks 517.20±10.66, 6 weeks 707.00 ±11.87) was significantly higher than in group B (2 weeks 153.60 ±7.16, 4 weeks 269.40±6.80, 6 weeks 279.20±6.53) (P <0.01).Vascular casting showed that in group A, significant blood vessels sprouted from all areas of the loop, espe-cially at the entrance of the arteriovenous pediele where the small tubes were densely interconnected.In group B, however, no blood vessels sprouted from the arteriovenous bundles and only some small vessels grew from the entrance and exit.Conclusions A vascularized coral model can be constructed by inserting an ar-teriovenous loop or an arteriovenous bundle, useful in vascular bone tissue engineering.The former, however, have stronger abilities to induce angiogenesis than the latter.     

烧伤患儿恐惧心理的护理

小儿烧(烫)伤是日常生活中十分常见的意外伤害,它不仅导致患儿身体遭受损害,还给患儿心理造成极大的恐惧感,导致了生活方式的改变。近年来,随着医学模式由生物医学模式向心理、社会、生物医学模式的转变,推广“以人为本,以病人为中心”的新型医疗模式,心理护理也得到了人们青睐     

黄芪注射液治疗幽门螺杆菌相关性胃炎的临床研究

目的:探讨黄芪注射液对幽门螺杆菌(HP)相关性胃炎的临床疗效。方法:将62例幽门螺杆菌相关性胃炎患者随机分为两组,治疗组和对照组各31例。对照组给予常规治疗(用法莫替丁、阿莫西林胶囊及复方灭滴灵片治疗4周),治疗组在对照组治疗基础上给予黄芪注射液20ml加入10%葡萄糖液250ml中静脉点滴,每日1次,4周为1个疗程,测定两组幽门螺杆菌阴转率,观察两组的临床疗效。结果:治疗组HP阴转率明显高于对照组(P＜0．05),治疗组临床疗效优于对照组(P＜0．05)。结论:黄芪注射液能提高幽门螺杆菌相关性胃炎的治愈率。     

带血管蒂逆行足背动脉岛状皮瓣修复拇趾软组织缺损骨外露

目的 探讨应用带血管蒂逆行足背动脉岛状皮瓣修复厨趾软组织缺损骨外露的优缺点。方法 对2例外伤致拇趾软组织缺损骨外露应用足背动脉的逆行岛状皮瓣修复厨趾软组织消灭创面，分析该皮瓣的可行性、实用性。结果：2例拇趾软组织缺损骨外露后应用带血管蒂逆行足背动脉岛状皮瓣修复均获成功。结论 带血管蒂逆行足背动脉岛状皮瓣修复足部厨趾软组织缺损骨外露操作简单，安全可靠，实用性强。     

汶川大地震后送伤病员病情特点及救治要点

2008年5月12日发生的汶川里氏8．0级地震造成37万余人受伤，近90％为肢体创伤病员。其中，10％-15％患者伴有颅面、胸腹部等处创伤。我院于2008年5月23日及28日分两批收治47名震区空运伤病员，其中骨科伤病员41例。本文针对我院接收的41例骨科伤病员的入院时病情特点、治疗措施及原则等作一简要总结。     

微粒子酶免疫测定乙肝病毒表面抗原的评价

目的：对微粒子酶免疫测定法（MEIA法）检测乙肝病毒表面抗原进行方法学评价。方法：采用美国雅培公司的AXSYM型化学发光仪对乙肝病毒表面抗原卫生部质控物、高浓度乙肝病毒表面抗原标本进行稀释测定，同时与ELISA法检测乙肝表面抗原进行比较。结果：微粒子化学发光法检测乙肝病毒表面抗原具有较高灵敏度，达0.1ng/ml；有较好的重复性和特异性。结论：微粒子酶免疫法对提高低含量乙肝表面抗原阳性检出率具有重要意义。     

血液灌流加透析抢救药物中毒及其护理对策

1　临床资料1 .1　一般资料5例中毒者 ,其中男 2例 ,女 3例 ,年龄最大 4 6岁 ,最小 1 4岁 ,平均年龄 2 9岁 ,均为口服中毒 ,其中有机磷中毒 2例 ,镇静剂中毒 1例 ,杀虫剂中毒 1例 ,药品不详 1例。就诊时昏迷 3例 ,嗜睡 1例 ,清醒 1例。 5例患者均经过急诊洗胃抢救后 ,到我科进行血液透析加灌流治疗。1 .2　材料与方法1 .2 .1　采用德国倍朗公司B -Braun -Dialog型透析机 ,山东廊坊爱尔血液净化器材厂的血液灌流器 ,血透管路 1套。方法 :用 5 0 g/L的葡萄糖 5 0 0ml灌满整个管路与灌流器 ,然后用肝素盐水 4 5 0 0ml冲洗灌流器 ,并轻轻拍打…     

成功救治大面积头皮撕脱伤伴失血性休克1例报告

病例女,21岁,因被米线机绞住头发撕脱头皮流血2h入院。受伤后大部分头皮被完全撕脱,入院时离体头皮随患者一同送入病房,撕脱头皮污秽,坏死。入院查体:T37.7℃;P125次/min;R23次/min;BP85/50mmHg。神志淡漠,烦躁不安。头部以绷带加压包扎,敷料及衣裤被鲜血浸透,头部敷料仍可见鲜