CK-7转录水平在乳腺癌外周血微转移检测中的应用

0 引言 乳腺癌是妇女最常见的恶性肿瘤之一,发病率呈逐年上升趋势,预后受诸多因素的影响,主要是全身转移.目前对微转移的检测方法和标志物较多,但没有公认的方法和标志物,逆转录结合荧光定量PCR(FQP-PCR)是一种检测微转移的有效方法,且敏感度较高.     

Isolation of cultured endothelial progenitor cells <Emphasis Type="Italic">in vitro</Emphasis> from PBMCs and CD133<Superscript>+</Superscript> enriched cells

Two isolation methods for sorting of endothelial progenitor cells(EPCs):from peripheral blood mononuclear cells(PBMCs)and CD133+ enriched cells were compared,by defining the cell morphology,phenotype,reproductive activities and function in vitro,to provide a reference for clinical application of EPCs.PBMCs from healthy subjects were used either directly for cell culture or for CD133+ sorting.The two groups of cells were cultured in complete medium 199(M199)for 7 to 14 days and the phenotypes of EPCs were an...     

T-PACS系统在构架新型医学教学体系中的应用

T-PACS教学系统是在放射信息系统(RIS)和医学影像存档与传输系统(PACS)的基础上,建成的用于医学影像学教学的多媒体网络教学系统.应用T-PACS系统终端进行影像学的教与学,是构建医学教学新体系的重要环节.由于其具有实用、高效、资源共享等优势,可将多种影像手段、多学科的知识综合示教,让学生对医学影像学知识有更直观的认识,从而对医学教学新体系的构建起到积极的意义.     

乳腺癌术后化疗患者的心理调查及分析

[目的]探讨乳腺癌术后化疗患者的心理特点,为临床治疗及护理工作提供科学依据。[方法]自制问卷调查表,对2007年6月～2009年12月某院住院接受化疗的68例乳腺癌患者分别进行心理调查。[结果]乳腺癌术后化疗患者普遍存在焦虑、恐惧、悲观等心理反应,年龄、受教育程度和经济状况不同,其心理障碍差异均具有统计学意义(P﹤0.05),患者随年龄的增大其心理障碍发生率降低,高学历患者心理障碍较为显著,患者经济状况越差,越易产生心理障碍。[结论]在乳腺癌术后化疗的过程中,患者易产生焦虑、恐惧、悲观心理等心理问题,医护人员应及时了解患者的心理活动,做好心理护理,改善患者生活质量,促进其尽快康复。     

Endothelial progenitor cells homing to the orthotopic implanted liver tumor of nude mice

This study investigated the "homing" phenomenon in hepatocellular carcinoma (HCC).The "homing" specificity of endothelial progenitor cells (EPC) by establishing an orthotopic implantation model in nude mice.EPCs harvested from the marrow cells were separated by density gradient centrifugation.Fluorescence microscope,flow cytometry (FCM) and double fluorescence staining with FITC-UEA-I and DiI-ac-LDL,were employed to identify the cells.4’,6-diamidino-2-phenylindole (DAPI) labelling and real-time PCR were used for detecting the expression of CD133 and chemokines to trace and observe the distribution of EPCs.Our results showed that the distribution rate of EPCs was obviously higher than that in other important organs and the negative control group.Detection of CD133 and chemokines yielded similar results in difference tissues.Our experiment confirmed that the chemotaxis of EPCs does exist in HCC.Moreover,HIF-1α,SDF-1 and VEGF might play important roles in the "homing" of EPCs in HCC.EPCs might be a potential candidate for targeting vector of HCC for gene therapy.