非淋菌性泌尿生殖道感染支原体检测及药敏试验

目的:了解解脲脲原体(Uu)和人型支原体(Mh)在非淋菌性泌尿生殖道感染中的致病作用及其体外药物敏感状况.方法:296例疑为非淋菌性泌尿生殖道感染的患者,运用微量稀释法进行支原体培养,测定解脲脲原体对10种抗菌药物的敏感性. 结果:101例(34.1%)支原体培养阳性,其中Uu阳性75例(25.3%),Mh阳性6例(2.0%),Mh＋Uu阳性20例(6.8%),女性(42.9%)支原体阳性率显著高于男性(28.8%).Uu对螺旋霉素均耐药,对氧氟沙星、四环素的耐药率均为37.3%,对交沙霉素的耐药率最低(8.0%), 对其他抗菌药物的耐药率依次为美满霉素(33.3%)、强力霉素(32.0%)、红霉素(30.7%)、左氧氟沙星(18.7%)、阿奇霉素(13.3%)、罗红霉素(10.7%).Uu对四环素类、大环内酯类和喹诺酮类有交叉耐药,以对四环素类和喹诺酮类的交叉耐药率最高(12.0%).结论:支原体的定期耐药性监测对临床用药有重要意义.     

人纤维介素在系统性红斑狼疮患者外周血单个核细胞中的表达及意义

我们用免疫组化方法检测了32例系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMNC)中人纤维介素(hfg12)蛋白表达情况，探讨hfg12在SLE发病中的作用及其与SLE疾病的关系。     

淋球菌MtrC融合蛋白单克隆抗体杂交瘤细胞株的建立和特性鉴定

目的：建立针对淋球菌MtrC膜蛋白的单克隆杂交瘤细胞株，制备相应的单克隆抗体（单抗），用于淋球菌耐药机制的研究和淋球菌耐药的临床检测。方法：用重组MtrC抗原免疫6周龄Balb/c小鼠，按常规淋巴细胞杂交瘤技术制备单抗。应用间接ELISA法和Western印迹法分别测定单抗亚类及其特异性。结果：建立了2株小鼠抗MtrC单抗杂交瘤细胞283和4F7，染色体数在95～103之间，能稳定分泌抗MtrC单抗，单抗类型为IgGl，腹水效价为1：10^5和1：10^6。结论：本研究建立的单抗在检测淋球菌MtrC膜蛋白时有高度特异性和敏感性。     

PI3K-Akt在皮肤鳞状细胞癌组织中的表达

【摘 要】 目的 检测皮肤鳞状细胞癌(SCC)组织中磷脂酰肌3-激酶(PI3K)及其下游分子蛋白激酶B(AKT)蛋白的表达,探讨其在SCC增殖及癌变中的作。 方法 采用免疫组织化学SP法检测41例SCC,11例正常皮肤组织石蜡标本中PI3K,AKT蛋白的表达。结果 SCC组织中PI3K,AKT蛋白的阳性表达率(65.85%和73.17%)明显高于正常皮肤(18.18%和0%,P<0.05)。SCC组织中PI3K的阳性表达率与AKT的阳性表达率呈正相关(r=0.418,P<0.05)。结论 PI3K-AKT通路可能在SCC的发生过程中起重要作用。     

阿奇霉素、氧氟沙星联合治疗解脲支原体所致非淋菌性尿道炎疗效观察

目的观察阿奇霉素和氧氟沙星联合治疗解脲支原体(UU)引起的非淋菌性尿道炎(NGU)的疗效。方法随机将286例UU引起的NGU患者分为108例口服阿奇霉素和氧氟沙星联合治疗组(观察组)和78例口服琥乙红霉素和氧氟沙星治疗组(对照组),疗程10天。结果观察组的治愈率(80.6%)极显著高于对照组(39.8%),P<0.01。结论阿奇霉素和氧氟沙星联合治疗UU引起的NGU具有较好的疗效,无明显副作用。     

淋球菌多重耐药性与mtrR基因点突变的关系

目的探讨多传递耐药（mtr）外排系统中mtrN基因点突变与淋球菌流行株多重耐药性的关系。方法应用K-B法和琼脂稀释法分离出55株淋球菌流行株。利用小量细菌基因组DNA快速抽提试剂盒取淋球菌DNA，PCR扩增16株淋球菌mtrR基因．并对扩增产物测序．比较淋球菌敏感株与多重耐药株的差异。结果淋球菌多重耐药株占76．36％（42／55）。4株敏感株和2株耐青霉素淋球菌无mtrR基因突变，10株多重耐药株均有mtrR基因点突变，表现为3种点突变形式：6株发生了第45位Gly（GGC→GAC）Asp，3株发生了第14位His（CAC→CGC）Arg．1株发生了第51化Phe（TTC→GTC）Val。结论淋球菌mtrR基因第45位Gly（GGC→GAC）Asp,14位His（CAC→CGC）Arg和第51位Phe（TTC→GTC）Val突变与淋球菌流行株的多重耐药性密切相关。     

解脲支原体及其合并人型支原体感染对7种抗生素敏感性的比较

目的 了解非淋菌性尿道炎(NGU)患者解脲支原体(UU)、人型支原体(MH)混合感染对7种抗生素的敏感性及其与解脲支原体单一感染的差异。方法 采用微量肉汤稀释法对UU、MH培养阳性的196例患者进行了7种抗生素的体外药物敏感性测定。结果 UU感染组与UU MH混合感染组对7种抗生素的敏感性的顺序基本相同，敏感性由高到低依次为：交沙霉素、强力霉素、美满霉素、司巴沙星、罗红霉素、氧氟沙星和阿奇霉素。UU MH混合感染组耐药总的发生率(97．62％)明显高于UU感染组(44．67％)，差异有极显著性(P＜0．01)。UU组和UU MH组中耐药率最高的分别为氧氟沙星(31．33％)和阿奇霉素(90．48％)。结论 UU MH混合感染后耐药性升高并发生耐药谱变化。     

In vitro Recombination and Identification of Mutated Fragment Corresponding to Regulation Region of mtrR Gene of Neisseria Gonorrhoeae

A site-directed mutant DNA fragment was synthesized and transfected into clinical Neisseria Gonorrhoeae(NG) stains to construct the transformants that contained the corresponding mutagenesis of regulation region of mtrR gene.According to the technique of gene splicing by overlap extension(SOEing),a DNA segment with specific mutagenesis was constructed by two-step polymerase chain reaction(PCR).The mutation fragments EF could be used for the next experiment in which the mutation NG strains were induced.By comparing the recombinant EF fragments to the corresponding DNA fragments of clinical NG strains,2 of these were not compatible completely.The results of sequencing revealed that there was a 9 bp deletion between the 45 to 54 inverted repeat sequence localized within the mtrR promoter.It can be confirmed that the fragments EF are the specifically designed mutant fragments.     

Expression of phosphorylated-STAT3 and osteopontin and their correlation in melanoma

The expressions of p-STAT3 and osteopontin in 22 cases of normal nevi and 43 cases of malignant melanoma were immunohistochemically detected, and the correlation between p-STAT3 and osteopontin in malignant melanoma and the correlations of p-STAT3 （or osteopontin） with invasion, metastasis and thickness of malignant melanoma were examined. The results showed p-STAT3 was expressed in 2 of 22 cases of normal nevi and 30 of 43 cases of malignant melanoma, while osteopontin was expressed in 3 cases of normal nevi and 29 cases of malignant melanoma. The expressions of p-STAT3 and osteopontin in melanoma were significantly higher than that in benign nevi. There existed significant correlations between the expression of p-STAT3 and that of osteopontin in melanoma. Furthermore, the expression rates of p-STAT3 were significantly higher in invasive or metastatic melanomas than that their non-invasive or non-metastatic counterparts, and the expression rates of osteopontin were significantly higher in invasive melanomas than that in non-invasive ones. It is concluded that p-STAT3 and osteopontin may play important roles in the pathogenesis of malignant melanoma.