11β-羟基类固醇脱氢酶Ⅰ型在不同年龄段 大鼠眼内的表达与分布

目的：观察糖皮质激素代谢酶11β-羟基类固醇脱氢酶Ⅰ型（11β-HSD1）在不同年龄Sprague-Dawlay （SD）大鼠眼内的表达与分布。方法：实验研究。将SD大鼠按年龄分为6组：1~2日龄新生组（PND1）、 5~6日龄组（PND5）、10~11日龄组（PND10）、14~15日龄刚开眼组（PND15）、20~21日龄组（PND20） 和8~9周龄成年组（M）,每组6只。采用免疫组织化学染色法（IHC）检测从新生、开眼到成年等不 同发育阶段SD大鼠眼组织内11β-HSD1蛋白的表达和分布情况,并通过Real time RT-PCR和Western blot进一步验证11β-HSD1在不同年龄段大鼠视网膜内的表达差异。组间数据比较采用单因素方差 分析或t检验分析。结果：IHC显示11β-HSD1在各年龄组SD大鼠眼角膜、虹膜、视网膜、脉络膜、 晶状体、睫状体等组织中均有表达,新生SD大鼠眼组织中的11β-HSD1阳性细胞数更多,染色更明 显。Real time RT-PCR结果显示,角膜内11β-HSD1 mRNA相对表达量在SD大鼠出生后10 d达到高 峰,SD大鼠开眼后开始逐渐下降,差异有统计学意义（F=8.40,P<0.001）。开眼前SD大鼠视网膜内 的11β-HSD1 mRNA相对表达量高于开眼后和成年,差异有统计学意义（F=83.50,P<0.001）。Western bolt进一步证实,新生大鼠视网膜内的11β-HSD1蛋白表达量高于刚开眼大鼠和成年大鼠,差异有统 计学意义（t=4.76,P=0.009;t=4.24,P=0.013）。结论：11β-HSD1在SD大鼠眼组织内广泛分布,其表 达量随年龄而变化,开眼前SD大鼠眼组织尚未分化发育成熟,其表达量较高,提示11β-HSD1可能 与SD大鼠眼组织的分化、发育和成熟有关     

Association between retinopathy, nephropathy, and periodontitis in type 2 diabetic patients: a Meta-analysis

AIM:To verify the association between retinopathy,nephropathy,and periodontitis in type 2 diabetic(T2 D)patients.METHODS:Several electronic databases were available for our comprehensive search including China National Knowledge Infrastructure(CNKI),Chinese VIP Information(VIP),Wanfang,Web of Science,ScienceDirect and PubMed and were queried for relevant citations(updated to Mar.2019).RevMan was utilized to perform Meta analysis and publication bias detection.After evaluation of the methodological quality of included studies,a fixed or random effect model was utilized to analyze data from included studies.RESULTS:A total of eight articles were finally included in this Meta analysis.In all 3987 subjects,there were 1207 T2 D patients accompanying with microvascular complications and 1734 patients with periodontitis as well.The Meta forest plot presented little heterogeneity of the eight studies(P<0.00001,I²=89%).The total effect demonstrated periodontitis was associated with overall microvascular complications(OR:1.96,95%CI:1.67-2.30,Z=8.25,P<0.00001).Subgroup investigations among the studies in Asian(OR:2.33,95%CI:1.91-2.85)and North American(OR:1.42,95%CI:1.08-1.86)populations confirmed the existed association between retinopathy,nephropathy,and periodontitis.While the strength of such associations between periodontitis and diabetic microvascular complications were more obvious in the Asians than North Americans.All the results indicated that periodontitis was associated with diabetic retinopathy(OR:3.77,95%CI:2.71-5.24),diabetic nephropathy(OR:1.55,95%CI:1.24-1.94)in T2 D patients.CONCLUSION:The periodontitis is associated with diabetic retinopathy,diabetic nephropathy among T2 D patients and further large sample size clinical trials are in need to confirm the findings.     

Study on pedestrian thorax injury in vehicle-to-pedestrian collisions using finite element analysis

Objective: To explore the relationship between the collision parameters of vehicle and the pedestrian thorax injury by establishing the chest simulation models in car-pedestrian collision at different velocities and angles.Methods: 87 cases of vehicle-to-pedestrian accidents, with detailed injury information and determined vehicle impact parameters, were included. The severity of injury was scaled in line with the Abbreviated Injury Scale (AIS). The chest biomechanical response parameters and change characteristics were obtained by using Hyperworks and LS-DYNA computing. Simulation analysis was applied to compare the characteristics of injuries.Results: When impact velocities at 25, 40 and 55 km/h, respectively, 1) the maximum values of thorax velocity criterion (VC) were for 0.29, 0.83 and 2.58 m/s; and at the same collision velocity, the thorax VC from the impact on pedestrian''s front was successively greater than on his back and on his side; 2) the maximum values of peak stress on ribs were 154, 177 and 209 MPa; and at the same velocity, peak stress values on ribs from the impact on pedestrian''s side were greater than on his front and his back.Conclusion: There is a positive correlation between the severity and risk of thorax injury and the collision velocity and angle of car-thorax crashes. At the same velocity, it is of greater damage risk when the soft tissue of thorax under a front impact; and there is also a greater risk of ribs fracture under a side impact of the thorax. This result is of vital significance for diagnosis and protection of thorax collision injuries.     

Utility of serum TNF-a,infliximab trough level,and antibody titers in inflammatory bowel disease

AIM:To assess tumor necrosis factor-a(TNF-a),infliximab(IFX)concentrations,and antibodies against IFX molecules in patients with inflammatory bowel disease(IBD)who develop loss of response,side effects,or allergic reaction during anti TNF-a therapy.METHODS:Blood samples of 36 patients with response loss,side effects,or hypersensitivity to IFX therapy(Group?Ⅰ)and 31 patients in complete clinical remission(GroupⅡ)selected as a control group were collected to measure trough serum TNF-a level,IFX,and anti-IFX antibody(ATI)concentration.We examined the correlation between loss of response,the development of side effects or hypersensitivity,and serum TNF-a,IFX trough levels,and ATI concentrations.RESULTS:The serum TNF-a level was shown to be correlated with the presence of ATI;ATI positivity was significantly correlated with low trough levels of IFX.ATIs were detected in 25%of IBD patients with loss of response,side effects,or hypersensitivity,however no association was revealed between these patients and antibody positivity or lower serum IFX levels.Previous use of IFX correlated with the development of ATI,although concomitant immunosuppression did not have any impact on them.CONCLUSION:On the basis of the present study,we suggest that the simultaneous measurement of serum TNF-a level,serum anti TNF-a concentration,and antibodies against anti TNF-a may further help to optimize the therapy in critical situations.     

CXCR3基因敲除小鼠慢性乙型肝炎病毒复制模型的建立

目的建立CXCR3基因敲除小鼠慢性乙型肝炎病毒复制模型。方法繁育CXCR3基因敲除小鼠,并抽提组织DNA进行聚合酶链反应及凝胶电泳鉴定基因型。CXCR3基因敲除小鼠纯合子9只与野生型C57BL/6小鼠9只同时高压水注射pAAV/HBV1.2质粒,按既定时间点采血检测HBsAg、HBeAg、HBV DNA,以及取肝组织行免疫组织化学检测HBcAg表达。结果本实验室繁殖的CXCR3基因敲除小鼠均为纯合子基因型。在pAAV/HBV1.2质粒转染后第1天,CXCR3基因敲除小鼠与野生型C57BL/6小鼠血清HBsAg水平分别为1134.69±244.42和1759.63±881.20(P=0.096);第4天分别为5305.29±1395.06和7493.29±658.63(P=0.003);第15天分别为1615.04±1187.16和1536.19±1046.02(P=0.905);第40天为229.45±79.27和228.19±295.02(P=0.996);第1天血清HBeAg水平分别为6.65±1.50和20.61±4.03(P=0.000);第4天为6.33±1.61和9.79±2.31(P=0.007);第15天为3.52±1.97和2.85±0.74(P=0.425);第40天为1.28±0.06和1.90±1.01(P=0.431);第10天血清HBVDNA水平分别为4.38±0.22 lgcopies/ml和6.56±0.16lgcopies/ml(P=0.008);第15天为4.41±0.88lgcopies/ml和5.69±0.04lgcopies/m(l P=0.177);第25天为4.48±0.04lgcopies/ml和6.44±0.16lgcopies/ml(P=0.004);第40天为3.66±0.45lgcopies/ml和5.20±0.28lgcopies/ml(P=0.055);两组血清HBV DNA持续存在,在转染后第40天仍为阳性。肝组织HBcAg持续阳性,在转染后第4天、15天和40天均呈高表达,但CXCR3基因敲除小鼠肝组织HBcAg表达较低。结论我们成功建立了CXCR3基因敲除小鼠慢性HBV复制模型,可用于进一步研究CXCR3基因及其配体与HBV感染的关系。     

P53蛋白在胰腺癌中的表达及其意义

ｐ５３蛋白在胰腺癌中的表达及其意义陈旭＊林永许东坡石铮福建医科大学附属第一医院外科（福州３５０００５）＊：现在福建省立医院外科关键词胰腺癌；ｐ５３蛋白；免疫组化；预后ｐ５３基因位于染色体１７ｐ１３上，它编码分子量为５３ｋＤ的核磷酸蛋白，具有转录因子...     

Quantitative Microbial Risk Assessment of Cryptosporidium and Giardia in Public Drinking Water in China

We aimed to assess the risks of Cryptosporidium and Giardia infections associated with drinking water for local residents, based on a quantitative microbial risk assessment, in three densely populated regions of China.     

树突状细胞对LAK细胞抗人鼻咽癌细胞的促进作用

本研究对人血树突状细胞联合ＬＡＫ细胞和ＩＬ－２对人鼻咽癌细胞株Ｈｅｐ－２的抗肿瘤活性进行了体外观察。实验分为ＬＡＫ组，ＬＡＫ＋ＤＣ组和ＬＡＫ＋ＤＣ＋ＩＬ－２组；效靶比例分别采用１０：１和２０：１二种。３７℃，５％ＣＯ２，饱湿条件下培养４８ｈ后，用中性红摄入比色法检测细胞毒活性。     

急性闭合性跟腱断伤治疗分析

目的探讨急性闭合性跟腱断伤的损伤机制,应用腓肠肌筋膜瓣翻转术治疗急性闭合性跟腱断伤的临床效果。方法对20例急性闭合性跟腱断伤患者采用腓肠肌筋膜瓣翻转术治疗的临床资料进行分析。结果20例获得随访6个月～3年,平均10个月。患者石膏去除后,半年经功能训练后均提踵有力无疼痛及跛行,有1例出现皮肤问题经换药后愈合。优良率达100％。结论对于急性闭合性跟腱断伤采用该手术方法治疗效果满意及同时修复腱周组织对于跟腱断伤愈合防止术后并发症具有重要意义。