HLA－DR在大肠癌的表达及其与转移和预后的关系

应用免疫组织化学方法对８３例不同分期的大肠癌及其正常粘膜组织和１２例大肠腺瘤进行Ⅱ类抗原ＨＬＡ－ＤＲ表达的研究。结果大肠癌ＨＬＡ－ＤＲ表达的阳性率为４７％，大肠腺瘤为１６．６％，而正常粘膜未见表达；ＨＬＡ－ＤＲ的表达与大肠癌的转移呈正相关，ＨＬＡ－ＤＲ表达强者其转移发生率低；ＨＬＡ－ＤＲ的表达与大肠癌的Ｄｕｋｅ＇ｓ分期及浸润深度有关，与细胞分化、肿瘤大小及位置无关；ＨＬＡ－ＤＲ是大肠癌的一个预后指标，表达阳性者其预后较好。     

Treatment of primary dysmenorrhea with herb-partitioned moxibustion plus ultra-short wave

Objective： To observe the clinical effect of herb-partitioned moxibustion plus ultra-short wave for primary dysmenorrhea （PD）. Methods： One hundred and thirty patients with PD were randomly divided into a treatment group and a control group, 65 cases in each group. Patients in the treatment group were treated with herb-partitioned moxibustion plus ultra-short wave, while patients in the control group were treated with oral administration of Ibuprofen sustained-release capsules. After completing three courses of treatments, the clinical effects were evaluated by professional researchers. Results： The recovery rate of the treatment group was 58.5%, and the total effective rater was 98.5%; versus 24.6% and 81.5% in the control group. The between-group differences were statistically significant （P〈0.05）. Conclusion： Herb-partitioned moxibustion plus ultra-short wave is an easy-to-operate and effective therapy for PD.     

ERK signaling mediates enhanced angiotensin II-induced rat aortic constriction following chronic intermittent hypoxia

Background Obstructive sleep apnea (OSA) has been recognized as an independent risk factor for systemic hypertension.The study investigated the functional consequences of chronic intermittent hypoxia (...     

Future application of hair follicle stem cells: capable in differentiation into sweat gland cells

Background Sweat glands (SGs) can not regenerate after complete destruction in the severe skin injury,so it is important to find a ideal stem cell source in order to regenerate functional SGs.Hair foll...     

Condyloma acuminatum and human papilloma virus infection in the oral mucosa of children

PURPOSE: The purpose of this study was to investigate the clinicopathological features of oral condylomas in children and condylomatous lesions of their mothers. Moreover, the authors sought to determine the mode of transmission of this disease and to find the genotype of human papilloma virus (HPV) in the children's oral condyloma. METHODS: Nine instances of oral condyloma acuminatum in children and lesions in their mothers were reviewed. Their HPV genotypes were evaluated by in situ hybridization (ISH). RESULTS: This study revealed that the lesions appeared during 3 years of age and the most common location was the hard and soft palate. Seven of the 9 mothers had experienced vulva and/or oral cavity condylomata during pregnancy. Social evaluation confirmed sexual abuse in 1 girl, and probable sexual abuse in another girl. The results of ISH demonstrated HPV 16/18 DNA being positive in 5 of the 9 cases, and HPV 6 and HPV 11, HPV 6 and HPV 16/18, HPV 6, and HPV 11 DNA being positive, respectively, in 1 case. HPV DNA types in mother-child pairs were not concordant. CONCLUSIONS: Oral condyloma acuminatum in children is probably induced by HPV 16/18. The mode of transmission by sexual abuse is the most likely route. Prenatal transmission of HPV to children is rare. This study provides further confirmation of possible different genotype and transmission in oral CA of children and adults.     

IL-0抑制转化生长因子β1诱导的大鼠肝星状细胞工型胶原合成作用观察

的探讨IL-10抗纤维化的可能机制。方法体外培养大鼠肝星状细胞系rHSC-99，分别用不同浓度的IL-10、TGFβ1干预和两者共同干预，用ELISA法检测肝星状细胞（HSC）的Ⅰ型胶原合成的影响。结果TGFp1显著增强HSC的Ⅰ型胶原的合成。IL-10单独干预对HSC的Ⅰ型胶原的合成无明显影响，但IL-10可显著抑制TGFβ1促进HSC的Ⅰ型胶原的合成。结论TGFβ1显著促进HSC的Ⅰ型胶原的合成，IL-10对TGFβ1的促HSC Ⅰ型胶原合成有抑制作用，这可能是IL-10发挥抗肝纤维化作用的途径之一。     

树突状细胞对LAK细胞抗人鼻咽癌细胞的促进作用

本研究对人血树突状细胞联合ＬＡＫ细胞和ＩＬ－２对人鼻咽癌细胞株Ｈｅｐ－２的抗肿瘤活性进行了体外观察。实验分为ＬＡＫ组，ＬＡＫ＋ＤＣ组和ＬＡＫ＋ＤＣ＋ＩＬ－２组；效靶比例分别采用１０：１和２０：１二种。３７℃，５％ＣＯ２，饱湿条件下培养４８ｈ后，用中性红摄入比色法检测细胞毒活性。     

Cryopreserved oocytes of infertile couples undergoing assisted reproductive technology could be an important source of oocyte donation: a clinical report of successful pregnancies

BACKGROUND: Surplus oocytes in assisted reproduction treatment cycles could be saved and donated to other couples. ICSI is usually performed for oocytes that have been stored frozen, considering possible exocytosis of cortical granules (CG). The unavoidability of ICSI merits further study. METHODS: We used a slow method to freeze excess oocytes from infertile couples. After thawing, oocytes were fertilized by either IVF or ICSI according to semen parameters. Some oocytes were examined for CG. RESULTS: Twenty-eight infertile couples cryopreserved a proportion of their oocytes and 12 thawed their oocytes. Three couples used their own oocytes, whereas nine donated their oocytes to nine other couples for 12 cycles. The survival rate from thawing was 90% (73/81). The fertilization rate using IVF (83%) was similar to ICSI (82%). Seven pregnancies (47% per cycle) were achieved; one used her own oocytes and six received donated oocytes. Five women delivered six babies including one set of twins. Two pregnancies aborted. The frozen-thawed oocytes (15/15) revealed no exocytosis of CG. CONCLUSIONS: To freeze oocytes of infertile couples undergoing assisted reproduction treatment may help other couples. Our successful experience may facilitate oocyte banks to become a reality. Both IVF and ICSI are valuable for frozen oocytes.     

Characteristic of the prevalence of J wave in apparently healthy Chinese adults

BACKGROUND: The J wave has been seen in idiopathic ventricular fibrillation, Brugada syndrome, and early repolarization syndrome. Although these conditions share some ECG features, the clinical consequences are markedly different. J wave presentation in healthy subjects is not a rare phenomenon, although its characteristics are poorly understood. The aim of this study was to investigate the prevalence and the characteristics of the J wave in apparently healthy Chinese adults. METHODS: The study was comprised of 1817 consecutive healthy Chinese subjects undergoing annual routine medical examination from April 2006 to July 2006, including 1131 men and 686 women. Routine medical examination including ECG was performed. J wave was defined as a wave on the ECG that followed the QRS complex with amplitude of at least 0.05 mV for 0.03 sec. RESULTS: The prevalence of the J wave in all subjects was 7.26%. The prevalence of the J wave in males was significantly higher than in females (10.52 vs. 1.89%, p<0.01). The incidence of the J wave in the inferior leads (II, III, avF), right precordial leads (V1-V3), and left precordial leads (V4-V6) was 4.57, 0.50, and 2.20%, respectively. The J wave prevalence in the inferior leads was significantly higher than in the left and right precordial leads (both p<0.05). Moreover, the prevalence of the J wave had a positive correlation with age in all subjects (r=0.78, p<0.01). CONCLUSIONS: J wave presentation on the ECG of apparently healthy people is not a rare phenomenon and is more likely to be found in the elderly, in males, and more frequently occurs in the inferior leads than in left and right precordial leads.