甲状腺机能亢进症患者血浆心钠素水平的动态变化

本文应用放射免疫法测定了50例甲亢患者及50例正常人血浆心钠素浓度,对其中25例甲亢患者测定了治疗前后血浆心钠素、肾素、血管紧张素Ⅱ浓度。结果表明甲亢患者血浆心钠素、肾素、血管紧张素Ⅱ明显升高,治疗后明显降低。甲状腺激素可能有促进心房肌细胞释放心钠素作用。     

桂利嗪-β-环糊精包合物的制备

目的:制备桂利嗪-β-环糊精包合物,并对包合物含量、溶解度进行了测定.方法:采用饱和溶液法制备桂利嗪-β-环糊精包合物.结果:经紫外光谱、相溶解度图法鉴定,桂利嗪与β-环糊精确已形成包合物,β-环糊精对桂利嗪的增溶倍数为3.33.结论:桂利嗪-β-环糊精包合物可使桂利嗪溶解度增加.     

糖尿病并发症的防治策略

研究显示,目前全球有糖尿病(DM)患者2.33亿,而且正以每年新发700万的速度猛增,估计到2025年全球将有3.80亿人患DM,其中增长最快的国家是中国、印度等发展中国家.     

HCCR-2反义核酸对肝癌HepG2细胞的作用

Objective To investigate the effects of antisense recombinant euraryotic expression vector of HCCR-2 on the proliferation and apoptosis of HepG2. Methods The antisense recombinant eukaryotic expression vector of HCCR-2 was constructed. The vector was stably transfected to the HepG2 cells, and positive clones were selected by G418 (antiseuse vector group), pIRES2-EGFP vector was transfected into the HepG2 cells in the same way (pIRES2-EGFP group). The conditions of the nontransfected HepG2 cells were used as control (HepG2 group). Changes in cell growth curve, cell cycle, cell apoptosis and morphology of HepG2 cells after the transfec-tion were detected by MTT method, flow cytometry and transmission electron microscopy, respectively. All the data were analyzed by one-way ANOVA and chi-square test. Results The expression level of HCCR-2 mRNA was down-regulated to 0.39±0.04 in antisense vector group, and the expression level of HCCR-2 mRNA in pIRES2-EGFP group and HepG2 group were 0.62±0.06 and 0.72±0.03, respectively, with significant difference among the 3 groups (F=43.701, P<0.05). The apoptotic rate of HepG2 cells in antisense vector group, pIRES2-EGFP grop and HepG2 group were 13.30%, 2.51% and 2.07%, respectively, with significant difference among the 3 group (χ2=6.793, 8.721, P<0.05). The growth of HepG2 cells in antisense vector group was retarded, and was blocked in G0/G1 stage. Conclusions The HCCR-2 antisense recombinant eukaryotic expression vector can inhibit the mRNA expression of HCCR-2 and promote the apoptosis of cells. HCCR-2 may be involved in cell regulation and the proliferation of hepatocellular carcinoma cells.     

鳃裂囊肿和鳃裂瘘的超声诊断与手术病理对照分析

目的 :分析鳃裂囊肿和瘘的声像特征及超声对该病变的诊断价值。方法 :对经手术病理证实的 6 8例鳃裂囊肿和瘘的声像图特征进行回顾性总结 ,与手术病理进行对照分析。结果 :第二鳃裂囊肿和瘘最多见占 85 .3%。其次是第一腮裂囊肿和瘘占 11.8%。第三、四鳃裂囊肿仅 2例 ,占 2 .9%。超声表现肿物大部分外形尚规则 ,边界清或尚清且包膜回声较强 ,内部回声的多样性与其病史和内壁衬里上皮有关。 75 % (5 1/ 6 8)的肿物位于胸锁乳突肌前缘或深面、颈动脉和颈内静脉的浅面。超声诊断该病的正确率为 88.2 %。结论 :高频超声对鳃裂囊肿和鳃裂瘘的诊断较为准确、可靠     

经尿道前列腺汽化电切术联合经尿道前列腺电切术治疗前列腺增生症的体会

目的探讨经尿道前列腺汽化电切术（TuVP）联合经尿道前列腺电切术（TURP）治疗良性前列腺增生的效果与优点。方法对68例前列腺增生症采用TUVP并用TURP治疗。结果68例良性前列腺珠增生症患者中术后随访0．6—3．0年，平均12．2个月。国际前列腺症状积分（IPSS）由术前27．4±3．5分下降至5．1±1．1分；残余尿由120．3±4．1ml减少至22．2±11．0ml；平均尿流率由6．8±3．1mVs增加至14．3±2．8ml／s。上述指标术前、术后相比，差异有显著性（P〈0．05）。结论经尿道前列腺汽化电切联合经尿道前列腺电切治疗前列腺增生症较开放手术具有适应证宽、痛苦少、出血少、恢复快、并发症少、疗效显著易于掌握等优点，值得临床推广应用。