心肌营养素1修饰的神经干细胞移植对癫(癎)持续状态大鼠海马损伤及苔藓纤维发芽的影响

Objective To observe the survival,migration and differentiation of grafted neural stem cells(NSCs)transfected with cardiotrophin-1(CT1)in hippocampus in status epilepticus(SE)rats,and investigate its effect on neuron loss and mossy fiber sprouting(MFS)in hippocampus of SE rats.Methods (1)Lithium-pilocarpine induced SE model rats were divided into 3 groups randomly:CT1-NSCs transplantation group(n=18);NScs transplantation group(n=18)and SE model group(n=18).Another 18 rats served as normal control group.Each group was further divided into 3 time points testing groups(n=6 at each point)corresponding to 1,4 and 8 weeks after transplantation respectively.(2)Under the confocal microscopy,the survival,migration and differentiation of the grafted cells were observed by immunofluorescenee.(3)Morphological changes and neuron loss in the hippocampal CA1 region were examined by Nissl staining.(4)MFS in hippocampal dentate gyrus in rats was obserred by Timm histochemistry.Results(1)At 4 and 8 weeks post-tmusplantation,the numbers of double-labeled NF-200 and EGFP pesitive cells in the CT1-NSCs group were significantly hisher than those in NSCs group.In the former group most of the grafted NSCs migrated away from the needle tract,but in the latter group,grafted ceHs remained at the transplantation site.(2)The numbers of neuron in the hippocampal CA1 region reduced gradually after SE.The numbers of neuron in the CA1 region in CT1-NSCs transplantation rats (68.85±11.49,60.89±12.17 and 51.51±13.34 in 1,4,8 weeks after transplantation respectivelv)were greater than that in NSCs transplantation rats(67.92±10.78,42.56±11.47 and 30.49±10.12).tvalue were 4.650 and 5.334 in 4 and 8 weeks after transplantation(P<0.05).(3)Aberrant MFS in the inner molecular layer of dentate gyrus was observed,and the scores of MFS gradually increased with timelapse.The scores of MFS in CT1-NSCs transplantation rats(0.77±0.04,2.48±0.89 and 2.39±0.82 in 1,4,8 weeks after transplantation respectively)were significant lower than that in NSCs transplantation rats (1.12±0.62,3.17±0.64 and 3.88±0.51,t=6.059,9.511 and 9.728,P<0.05).Conclusions CT1 could promote the survival,migration and differentiation of engrafted NSCs in hippocampud in SE rats.Engrafted NSCs transfected with CT1 have effect on repair of the injured hippocampus,and could inhibit hippocampus MFS in SE rats.     

54例小儿脑外伤急性期EEG与CT比较

小儿由于自我防护意识的欠缺,不同程度的脑外伤发病率较高。现对2005年1月至2006年1月我院就诊的54例脑外伤后急性期的患儿进行CT及脑电图(EEG)检查,以探讨小儿脑外伤急性期EEG特征及与CT的相互关系。现报道如下。1资料与方法1.1资料54例中男41例,女13例,年龄8个月~14岁,其中>3     

神经皮肤黑变病1例

神经皮肤黑变病是皮肤神经综合征的一种,临床罕见。我科近期接诊1例神经皮肤黑变病患儿,现报告如下。1临床资料患儿男,6个月。因“频繁抽搐发作1周”求诊。患儿系第一胎第一产,足月自然分娩,产程顺利,Apgar评分1分钟、5分钟均评10分。出生即见全身皮肤大量黑色素斑。生长发育类     

心肌营养素1修饰的神经干细胞移植对癫(癎)持续状态大鼠海马损伤及苔藓纤维发芽的影响

Objective To observe the survival,migration and differentiation of grafted neural stem cells(NSCs)transfected with cardiotrophin-1(CT1)in hippocampus in status epilepticus(SE)rats,and investigate its effect on neuron loss and mossy fiber sprouting(MFS)in hippocampus of SE rats.Methods (1)Lithium-pilocarpine induced SE model rats were divided into 3 groups randomly:CT1-NSCs transplantation group(n=18);NScs transplantation group(n=18)and SE model group(n=18).Another 18 rats served as normal control group.Each group was further divided into 3 time points testing groups(n=6 at each point)corresponding to 1,4 and 8 weeks after transplantation respectively.(2)Under the confocal microscopy,the survival,migration and differentiation of the grafted cells were observed by immunofluorescenee.(3)Morphological changes and neuron loss in the hippocampal CA1 region were examined by Nissl staining.(4)MFS in hippocampal dentate gyrus in rats was obserred by Timm histochemistry.Results(1)At 4 and 8 weeks post-tmusplantation,the numbers of double-labeled NF-200 and EGFP pesitive cells in the CT1-NSCs group were significantly hisher than those in NSCs group.In the former group most of the grafted NSCs migrated away from the needle tract,but in the latter group,grafted ceHs remained at the transplantation site.(2)The numbers of neuron in the hippocampal CA1 region reduced gradually after SE.The numbers of neuron in the CA1 region in CT1-NSCs transplantation rats (68.85±11.49,60.89±12.17 and 51.51±13.34 in 1,4,8 weeks after transplantation respectivelv)were greater than that in NSCs transplantation rats(67.92±10.78,42.56±11.47 and 30.49±10.12).tvalue were 4.650 and 5.334 in 4 and 8 weeks after transplantation(P<0.05).(3)Aberrant MFS in the inner molecular layer of dentate gyrus was observed,and the scores of MFS gradually increased with timelapse.The scores of MFS in CT1-NSCs transplantation rats(0.77±0.04,2.48±0.89 and 2.39±0.82 in 1,4,8 weeks after transplantation respectively)were significant lower than that in NSCs transplantation rats (1.12±0.62,3.17±0.64 and 3.88±0.51,t=6.059,9.511 and 9.728,P<0.05).Conclusions CT1 could promote the survival,migration and differentiation of engrafted NSCs in hippocampud in SE rats.Engrafted NSCs transfected with CT1 have effect on repair of the injured hippocampus,and could inhibit hippocampus MFS in SE rats.     

发热诱导的学龄儿童难治性癫痫性脑病最新进展

发热诱导的学龄儿童难治性癫痫性脑病（FIRES）是近年引起广泛关注的一类综合征,常由发热诱导、好发于既往健康的学龄儿童,表现为难治性癫痫及癫痫持续状态,抗癫痫药物无效,病情危重,死亡率极高。幸存者多合并严重认知障碍及难治性部分性癫痫。病因及发病机制不详、治疗困难、预后不良。本文对近年来有关 FIRES 发病机制、临床特征、诊断及治疗的文献进行综述。     

儿童结核性脑膜炎抗结核治疗中发生类赫反应的危险因素

目的:探讨儿童结核性脑膜炎(TBM)抗结核治疗中出现类赫反应(PR)的临床特征、危险因素及对预后的影响。方法:回顾性分析2013年1月至2018年12月在遵义医科大学附属医院儿科收治的TBM患儿的临床资料及随访情况;根据是否发生PR分组,采用单因素分析选出PR的影响因素后,引入多因素 Logistic回归分...     

特发性儿童枕叶癫痫伴睡眠期癫痫性电持续状态

通常认为特发性枕叶癫痫呈良性经过,不伴明显的认知功能障碍及智力障碍.但是,近年国外有报道质疑枕叶癫痫,尤其是晚发型枕叶癫痫的良性预后,患儿抗癫痫药难以控制,还可存在明显的认知障碍[1],尤其是脑电图(EEG)显示枕区持续性电发放者.但国内尚未见特发性枕叶癫痫伴睡眠期癫痫性电持续(electrical status epilepticus during sleep,ESES)状态的报道.本文报道2例特发性枕叶癫痫伴ESES状态,均存在明显的认知及学习障碍.     

心肌营养素1对谷氨酸诱导神经干细胞凋亡的作用

背景：心肌营养素1属于白细胞介素6细胞因子家族,近年发现其可能对中枢神经系统有重要保护作用。目的：探索重组腺病毒心肌营养素1对谷氨酸诱导神经干细胞凋亡的保护作用,为脑损伤提供新的治疗措施。方法：取新生Wistar大鼠海马神经干细胞,第2代培养第5天用于实验,神经干细胞分为3组：谷氨酸组、重组腺病毒心肌营养素1组及对照组,利用四唑蓝比色了解细胞存活情况及原位缺口末端标记技术检测细胞凋亡。结果与结论：谷氨酸组神经干细胞呈现明显形态改变,重组腺病毒心肌营养素1组未见类似改变,在谷氨酸暴露后12,24,48及72hMTT吸光度值均低于心肌营养素1组,而细胞凋亡率均高于心肌营养素1组。与其他两组比较,对照组相同时间点A值增高,细胞凋亡率降低。提示,心肌营养素1对谷氨酸损伤的神经干细胞具有保护作用,可以抑制神经干细胞凋亡,促进其存活和增殖。     

Adv-CT1转染对神经元的作用

目的观察重组腺病毒心肌营养素1（recombinant adenovirus cardiotrophin-1,Adv-CT1）对谷氨酸（glutamate,Glu）诱导损伤神经元的保护作用。方法分离、培养新生Wistar大鼠神经元细胞,将细胞分为单纯谷氨酸损伤组、Adv-CT1转染组及正常对照组;利用台盼蓝染色技术检测神经元细胞存活率;采用TUNEL原位细胞凋亡检测技术检测神经元细胞凋亡率。结果谷氨酸损伤第1、2及3天,单纯谷氨酸损伤组细胞存活率（77.4%、72.7%、67.0%）较Adv-CT1转染组（84.8%、78.5%、74.0%）明显降低（P〈0.05）;单纯谷氨酸损伤组细胞凋亡率（2.7%、5.5%、6.8%）较Adv-CT1转染组（1.5%、2.0%、3.2%）明显增高（P〈0.05）。结论 CT1转染可减少谷氨酸损伤诱导的神经元细胞凋亡发生,促进损伤神经元细胞存活,对损伤的神经元细胞具有保护作用。     

心肌营养素1对谷氨酸诱导神经干细胞凋亡的作用**

背景：心肌营养素1属于白细胞介素6细胞因子家族,近年发现其可能对中枢神经系统有重要保护作用。 目的：探索重组腺病毒心肌营养素1对谷氨酸诱导神经干细胞凋亡的保护作用,为脑损伤提供新的治疗措施。 方法：取新生Wistar大鼠海马神经干细胞,第2代培养第5天用于实验,神经干细胞分为3组：谷氨酸组、重组腺病毒心肌营养素1组及对照组,利用四唑蓝比色了解细胞存活情况及原位缺口末端标记技术检测细胞凋亡。 结果与结论：谷氨酸组神经干细胞呈现明显形态改变,重组腺病毒心肌营养素1组未见类似改变,在谷氨酸暴露后12,24,48及72 h MTT吸光度值均低于心肌营养素1组,而细胞凋亡率均高于心肌营养素1组。与其他两组比较,对照组相同时间点A值增高,细胞凋亡率降低。提示,心肌营养素1对谷氨酸损伤的神经干细胞具有保护作用,可以抑制神经干细胞凋亡,促进其存活和增殖。