神经生长因子干预实验性自身免疫性脑脊髓炎幼年大鼠脑神经干细胞的变化

BACKGROUND:Nerve growth factor is a neurotrophic factor that is involved in the cell regulation and promotes the proliferation of neural stem cells. OBJECTIVE:To observe the effect of nerve growth factor on neural stem cells of experimental autoimmune encephalomyelitis rats. METHODS:Wistar rats, 3 weeks of age, were randomly divided into control group, encephalomyelitis model group, nerve growth factor treatment group. Rat models of experimental autoimmune encephalomyelitis were made in the latter two groups. On the day of onset, rats in the nerve growth factor treatment group were given intraperitoneal injection of 1 000 U/kg nerve growth factor for 7 continuous days, and rats in the other two groups given no treatment. Effects of nerve growth factor on clinical symptoms of model rats were observed. Expression of Brdu in the subependymal zone and Brdu⁺/GFAP⁺ expression in the cortex were detected. RESULTS AND CONCLUSION:Rat models of experimental autoimmune encephalomyelitis appeared to have encephalomyelitis symptoms successively at the beginning of 10-day immunization, while rats in the control group had no symptoms of encephalomyelitis. In the model group, glial cell hyperplasia, inflammatory cell infiltration, damage to vascular endothelial cell proliferation, and perivascular aggregation of inflammatory cells in the rat brain were found, while no abnormal changes in the rat brain were found in the control group. Compared with the model group, the expression of Brdu in the subependymal zone and Brdu⁺/GFAP⁺ expression in the cortex were both higher in the model group (P < 0.05), and these expressions were also higher in the nerve growth factor treatment group than the model group at 7 and 21 days after onset (P < 0.05). To conclude, these findings suggest that the rat model of autoimmune encephalomyelitis can be successfully established, and nerve growth factor treatment can improve clinical symptoms of experimental autoimmune encephalomyelitis rats by promoting the proliferation and differentiation of neural stem cells into astrocytes.     

小切口复位植骨内固定治疗跟骨骨折80例临床分析

跟骨骨折临床常见,占全身骨折的2%。如处理不当,可产生功能障碍或较严重的手术并发症。保守治疗更难以达到令人满意的效果,遗留行走疼痛、跛行步态等,对工作生活影响较大。本院自1997—2010年采取小切口复位植     

HPLC测定复尔康注射液中维A酸在家兔体内的药动学

目的：采用高效液相色谱法（HPLC）测定复尔康注射液中维A酸的血药浓度,计算药动学参数,并与维A酸对照品溶液比较其药动学特征。方法：色谱柱：Dikma Kromasil C18（250min×4．6mm,5μm）;流动相：乙腈-0．1％磷酸溶液;流速：1．0ml·min^-1;检测波长：355nm;柱温：30℃;进样量：20μl。结果：复尔康注射液中维A酸和维A酸对照品溶液在家兔体内均符合开放二室动力学模型特征。复尔康注射液中维A酸的t1/2α=10．61min,t1/2β=99．44min,K10=0．022min^-1,AUC=257．15μg·min·ml^-1,CL=0．022mg·kg·min^-1;维A酸对照品溶液的t1/2α=8．61min,t1/2β=54．06min,K10=0．033min^-1,AUC=174．87μg·min·ml^-1,CL=0．032mg·kg·min^-1。结论：与维A酸对照品溶液相比,复尔康注射液中维A酸的消除速率减慢,生物利用度增加,在体内滞留时间延长。     

核因子κB与肝脏疾病

细胞核因子κB又称κ基因结合核因子（nuclear factor kgene binding,NF—κB）,是一种广泛存在于细胞中的具有多向调节作用的蛋白质分子,参与细胞激酶、趋化因子、生长因子、细胞黏附因子及早期反应的蛋白质分子基因的转录,其活性受到一个NF—κB抑制蛋白（inhibitor of κB,IκB）的抑制。近年来研究结果表明：NF—κB能介导广泛的生物学作用,参与多种疾病的发生发展过程,尤其在肝脏疾病中发挥着不可忽视的作用,本文就核因子κB与肝脏疾病之间的关系作一综述。     

辛伐他汀对大鼠创伤性静脉血栓形成的影响

目的：观察辛伐他汀对大鼠创伤性深静脉血栓形成情况以及血栓形成后炎症标志物的变化情况,以探索辛伐他汀的预防血栓形成及抗炎机制。方法取SD大鼠24只随机分为干预组和对照组,每组12只。干预组造模前连续1周喂服普通饲料,同时喂服辛伐他汀,对照组仅喂服普通饲料。2组均造亚急性大鼠创伤性静脉血栓形成模型。造模成功后干预组继续喂服普通饲料同时喂服辛伐他汀,观察组则继续喂服普通饲料。1周后分别采取各组大鼠静脉血行血常规、C反应蛋白（C reactive protein,CRP）及白介素-6（interleukin-6,IL-6）检测,然后处死大鼠,观察大鼠血栓形成情况,比较2组血栓形成的相对危险度（RR）,同时比较炎症反应情况。结果对照组比干预组血栓形成的相对危险度（RR）为2.25倍,干预组白细胞、CRP及IL-6显著低于对照组（P＜0.05）。结论辛伐他汀能减轻大鼠下肢静脉血栓的发生率,对大鼠下肢静脉血栓有治疗作用,能明显减轻炎症反应,改善大鼠下肢静脉血栓形成的预后。     

炎症与下肢深静脉血栓形成的关系研究

目的观察炎症标志物与下肢深静脉血栓（DVT）形成的关系。方法将确诊的下肢DVT患者42例为观察组,另随机选取就诊的健康体检者40例为对照组。两组均采集静脉血进行G反应蛋白（CBP）、红细胞沉降率（ESR）、纤维蛋白（原）降解产物（印P）及血常规检测。结果DVT患者CBP、ESB、FDP、白细胞、中性粒细胞、单核细胞水平明显升高,与对照组比较差异有统计学意义（P〈0.05）。结论下肢DVT表现出CBP、ESR、白细胞等炎症标志物的水平变化,临床可结合以上指标作为DVT的辅助诊断,有助于发现DVT高危人群,预警DVT的发生。     

乳必泰胶囊药效学研究

目的：探讨乳必泰胶囊的药效学。方法：通过大鼠乳腺增生模型及其它试验观察本品对乳腺增生的抑制作用及其它药理作用。结果：乳必泰胶囊能明显抑制乳腺组织增生，降低血清雌二醇水平，减少乳头直径及子宫系数，能明显抑制角叉菜胶引起的足肿胀。结论：这些作用说明乳必泰胶囊用于治疗乳腺增生，可能对患的症状有一定缓解作用。     

荆黄汤熏洗液抗炎镇痛与活血化瘀作用研究

目的：观察荆黄汤熏洗液的抗炎镇痛与活血化瘀作用。方法：分别采用鼠耳肿胀法、大鼠足爪肿胀法观察荆黄汤熏洗液的抗炎作用和采用小鼠热板法、扭体法观察其镇痛效应;测定血瘀模型大鼠血流变指标及小鼠凝血时间考察其活血化瘀作用。结果：荆黄汤熏洗液能减轻二甲苯致小鼠耳廓肿胀和蛋清所致大鼠足跖肿胀,提高小鼠对热板性疼痛的痛阈值,减少扭体反应的平均次数;荆黄汤熏洗液能明显降低血瘀模型大鼠的全血粘度、血浆粘度、血小板聚集率、红细胞压积和血沉,延长小鼠的凝血时间。结论：荆黄汤熏洗液具有抗炎镇痛和活血化瘀作用。     

艾滋病口腔炎含漱液对口腔黏膜与皮肤安全性的研究

目的 通过动物口腔黏膜、皮肤给药安全性试验,观察艾滋病口腔炎含漱液的安全性,为临床用药提供安全性参考依据.方法 按照国家颁发的皮肤安全性试验方法,将含漱液以0.425 g/ml 和1.7 g/ml分别对白家兔进行急性毒性试验、口腔黏膜与皮肤刺激性试验,对豚鼠进行皮肤过敏性试验.用药后分别观察受试动物呼吸和中枢神经系统症...