红藤脂溶性成分的分离和鉴定

从红藤Sargentodoxa cuneata(Oliy.)Rend.et Wils.的脂溶性部分分离到九种成分,经理化性质与波谱数据分析,分别鉴定了它们的结构。其中香荚兰酸(Ⅰ)、原儿茶酸(Ⅱ)、大黄酚(Ⅲ)和新化合物对-香豆酸-对-羟基苯乙醇酯(Ⅳ)首次从红藤中分离到。     

丝棉木的三萜成分研究Ⅰ

从药用植物丝棉木Euonymus bungeanus,Maxim的茎木部分离得八种三萜(Ⅰ～Ⅷ)。经波谱和其他理化性质分析,确定Ⅰ为moronic acid,Ⅱ为雷公藤内酯乙(wilforlideB),Ⅲ为雷公藤内酯甲(wilforlide A),Ⅳ为齐墩果酸(oleanolic acid)和Ⅷ为3β,22α-二羟基-△~(12)-齐墩果烯-29-羧酸。上述成分均为首次从丝棉木中分得。此外还得到没食子酸。     

红藤水溶性提取物的抗心肌缺血研究

用异丙肾上腺素诱发大鼠心肌坏死模型对红藤水溶性提取物红藤甲素、丙素及多糖进行比较研究。连续两天给药(每天5mg/kg)并记录EKG变化,第三天开胸取心进行病理学检查。EKG和病理学检查结果均表明,三种成分中多糖对缺血心肌的保护作用最强,丙素有微弱的保护作用,而甲素未见有统计学的显著差异。     

中药槐花米的化学成分研究[Ⅰ]槐花米甲素(1)

Hwai-Hua-Me has long been used as insecticide,dye-stuff,nutriment,heamosta- tic agent as well as remedies for haemorrhoids and brain hyperemia (apoplexy). Hitherto six substances have been isolated by the author from a sample ob- tainable in Shanghai. The presant paper deals with the study of Sorphorine-A,a yellowish crystal- line subtance.The yield is about 14%.The sample was first extracted with dilute alcohol,then washed with ether,acetone and petroleum ether,and the Product was recrystallized from alcohol and hot water,and then reated with methyl alcohol and ether mixture in order to remove Sorphorn-B,Sorphorin-C & etc.The substance thus obtained gives the following: m.p.180-182°(bulbing),185-188°(Changing forom),199-203°(Sintering with plastic appearance) and decomposing at 245-250°). [a]_D²⁴=+12°(0.5% C₂H₅OH);+5°(% CH₃OH). Mol.formula:C₂₉H₃₆O₁₇. Crystallization of water:7.16;7.33%;CH₂₉H-(36)O₁₇·3H₂O. Crystallizion of methyl alcohol:4.86%,C₂₉H₃₆O₁₇·CH₃OH. Crystallization of ethyl alcohol:6.57%,C₂₉H₃₆O₁₇·C₂H₅OH. Solubility:cold water(1:60,000);boiling water (1:167). cold methyl alcohol(1:1,080);boiling methyl alcohol(111:100). cold ethyl alcohol(1:600);boiling ethyl alcohol(3:5). cold acetone(1:600);boiling acetone(1:350). Pyridine(1:12). cold dioxan(1:12);boiling dioxan(1:15). cold ethyl acetate(trace);boiling ethyl acetate(small). cold amyl alcohol(slightly);hot amyl alcohol(slightly). Reactions:1.Tollen's reagent:black ppt. 2.Fehling's solution:no reaction. 3.HCl+Mg+C₂H₅OH:reddish purple coloration. 4.HAC+Mg+C₂H₅OH:greenish fluorescence. 5.Hydrolysis(1:100 HCl): a.yellowish brown crystals(yield 59%),306-308°(blackens),310-311° (melts),C₁₇H₁₃₍₁₄₎O₉₍₁₀₎. b.acetone Insoluble osazone:m.p.215-217°(D). c.acetone soluble osazone:m.p.188-189. 6.FeCl₃ solution:dark green solution. 7.Lead acetate solution:Lemon-yellow ppt. 8.Basic lead acetate solution:yellowish Orange ppt. 9.Hydrolyzed product:yellowish brown crystal. a.FeCl₃ solotion:bark green solution. b.Lead acetate:yellowish orange ppt. c.Tollen's reagent:black ppt. d.Fehling's solution:no reaction. e.Acetate,C₂₇₍₂₈₎ H₂₃₍₂₄₎O₁₄:m.p.198-203°,CH₃CO%=44.4%;hydro- lyzed with KOH,give greenish yellow crystals,yield about 98% with m.p.310-312°(D). f.Methyated:(1)m.p.300-302° greenish yellow. (2)m.p.151-152° slight yellowish white. The spectrophotometric determination: As show in Fig.Vl (1) Hwai-hua-menine-A exhibits absorption at E_1cm^1% 258 mμ =353;E_1cm^1% 364 mμ=277.8(95% ethyl alcohol+1% 0.2N HAC),(Conc.1.551 mg/100CC.)(Beckman DU). (2) aglucone of Hwai-Hua-Menine-A exhibits absorpotion at: E_1cm^1% 258 mμ=660;E_1c%^1% 375 mμ=550. The paper chromatography determination: Solvent:n-butanol:acetic acid:water=4:1:5 for 10 hours. R_f.=0.68 (0.71-0.72) Aglucone:R_f=0.75.(0.73) Acetic acid:water(6:4 V/V), R_f.=0.71,0.72;Aglucone:R_f=0 The active Oh-groug of aglucone(Tschugajiff Zerrmettinoff's method 26.8%.) The mixed m.p.of rutin(obtained from leaf and stem of Fagoyprum esculentuni, Moench 蕎麦茎叶) and Hwai-Hua-Menine-A:154°(contract),180-190°(syrup), 240-250°(D). It may be concluded from the above results,that the compound is neither rutin, nor its homologues.     

红藤水溶性提取物对家兔实验性心肌梗塞的影响

红藤水溶性提取物给心肌梗塞(简称心梗)家兔静脉注射(简称静注)100mg/kg体重,能使已抬高的ST段显著下降,改善结扎左冠状动脉前降支(简称左前降支)所致的心肌乳酸代谢紊乱,并缩小心梗范围。