两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

医院学科综合评价研究的应用与前景

在介绍医院学科综合评价的内容与意义的基础上，对国内外医院学科综合评价研究的现状、医院学科综合评价研究的一般步骤、医院学科综合评价的应用等进行了综述。指出：科学构建学科评价指标体系是学科评价的关键，规范收集学科评价数据是学科评价的基础，评价实施中要合理选择学科评价组织形式，有效利用学科评价结果。同时对医院学科综合评价的前景进行了展望。     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

无偿献血血液报损原因分析

20 0 2年 1月～ 2 0 0 2年 6月 ,本市街头无偿献血 80 1 6人份 ,报损血液 5 79份 ,占 7.2 2 %。如何加强对无偿献血各环节的有效监控 ,尽量减少血液浪费 ,对于进一步保证血液质量尤为重要。笔者就此分析如下。对象与方法1　对象　 2 0 0 1年 1月～ 2 0 0 2年 6月无偿献血 80 1 6人份。2　方法　无偿献血者经献血前咨询、体格检查初步合格后 ,取手指血用全血金标法快速检测 HBs Ag,结果为阴性者采血 ,并按卫生部要求进行初检、复检。每月对血站采供血及报损情况进行统计上报 ,并进行质量控制。结果与讨论按献血者健康检查标准要求 ,经体格…     

关怀因素调查-护士长关怀量表的汉化及信效度研究

目的译制英文版关怀因素调查-护士长关怀量表(Caring Factor Survey-Caring of Manager,CFS-CM),并对中文版信效度进行评价。方法遵循Brislin翻译回译原则进行翻译,采用专家咨询法对其进行文化调适,形成中文版CFS-CM。采用整群抽样对北京市某三级甲等医院1 168名临床护士进行调查,评价量表的信效度。结果中文版CFS-CM的Cronbach’sα系数为0. 961,重测信度为0. 922;各条目间以及与总分的相关系数分别为0. 812～0. 898;各条目的内容效度指数为0. 830～1. 000,量表平均内容效度指数为0. 983。探索性因素分析提取的1个公因子累积方差贡献率为70. 389%;验证性因素分析的各项拟合指数结果为:/df=5. 748,GFI=0. 920,AGFI=0. 874,RMSEA=0. 096,NFI=0. 964,CFI=0. 970,IFI=0. 970,TLI=0. 961均在可接受范围内,适配较好。不同年龄、护龄及不同职称的护士长关怀总分差异均有统计学意义(均P 0. 05)。结论中文版CFS-CM具有较好的信度和效度,可以作为评价我国护理管理者对护士关怀程度的工具。     

复发性流产风险基因的临床应用价值

复发性流产(RSA)是1%～5%育龄妇女的常见不良妊娠结局,其病因复杂,发病机制尚不明确。目前仍有约50%反复流产原因不明,RSA可能受到多种外源性和内源性因素的影响,其中遗传因素倍受关注。本文旨在探索RSA的相关风险基因,包括与血栓形成相关的F5、F2、MTFHR等基因、与免疫反应相关的HLA-G、CTLA4、IL-6等基因、与着床和胎盘形成相关的VEGFA、NOS3、MMP2等基因,以及与激素及其他相关的ESR1、ESR2、CYP1A1等基因,探讨基因多态性与RSA发生风险的关联。通过总结与RSA发生风险相关的易感基因,探索遗传因素在RAS风险预测的临床价值,有助于评估RSA的影响因素和发病机制,为建立精准多基因遗传风险评分(PRS)模型提供基础,并从遗传学的角度指导RSA高危人群的筛查及防控。     

组蛋白甲基转移酶SETD2在肾细胞癌发生中的作用机制

肾细胞癌是一种具有复杂遗传背景的癌症,研究表明,异常的表观遗传调控是肾细胞癌发生的主要原因之一。组蛋白甲基转移酶SETD2是一种重要的表观遗传分子,并且在肾细胞癌中具有很高的突变率。为了探究SETD2在肾细胞癌发生与发展中的作用和机制,该研究利用肾脏特异性敲除SETD2的基因小鼠模型与肾细胞癌表型分析,发现在VHL缺失的条件下,SETD2的缺失能够导致肾细胞癌的发生。转录组测序结果显示,SETD2的敲除导致了Wnt/β-catenin与FoxO信号通路的激活。该研究为肾细胞癌的治疗提供了新的靶点和思路。     

胡椒碱对HepG2细胞胰岛素抵抗模型糖代谢AMPK信号通路上游靶点干预机制的研究

探讨胡椒碱对胰岛素抵抗(insulin resistance,IR)细胞模型糖代谢紊乱的作用及干预AMPK信号通路上游靶点的分子机制。通过脂肪乳诱导HepG2细胞构建胰岛素抵抗模型。葡萄糖氧化酶(GOD-POD)法检测各组葡萄糖消耗量;酶联免疫吸附测定(ELISA)法检测上清液中脂联素(adiponectin,APN)、瘦素(leptin,LEP)的水平;荧光定量PCR法检测APN,LEP mRNA的表达水平,Western blotting法检测AMPKα,р-AMPKα,瘦素受体(LepR),脂联素受体1(AdipoR1)和脂联素受体2(AdipoR2)蛋白的表达。结果显示,胡椒碱与降糖药罗格列酮、AMPK激动剂AICAR均能明显提高胰岛素抵抗细胞模型的葡萄糖消耗量,升高脂联素水平、增加脂联素mRNA、脂联素受体1蛋白的表达,也增加AMPKαmRNA和р-AMPKα蛋白表达;同时降低瘦素mRNA和瘦素受体蛋白的表达。结果表明,胡椒碱能显著改善胰岛素抵抗细胞模型糖代谢紊乱,其作用机制可能通过调控上游脂联素和瘦素的表达,从而激活AMPK信号通路。     

心音监测技术临床应用的研究进展

心脏疾病是造成人类因病致残和死亡的常见疾病,心音是指心脏瓣膜打开或关闭、通过瓣膜口的血液流动及血液流经心室壁大动脉壁等引起振动所产生的声音。心音与心脏结构和功能具有明显相关性,作为一种无创辅助诊断工具,心音监测技术在心血管疾病的诊断和治疗中发挥着重要作用。因此,近年来心音监测技术逐渐受到国内外学者重视,相关临床应用方面的研究也越来越多。本文综述了心音监测技术临床应用的相关文献,并对其在新兴的移动医疗保健领域及围术期监测领域的应用进行展望。     

灾害救援医学工程技术体系的构建与实践

我国灾害事故易发多发,救灾的关键是救人.笔者结合救援研究与实践经验,提出了以航天、航空、地面、隧道、水域为研究场景的救援空间维度,即"天、空、地、隧、水";以检查检验、监测监护、搜索营救、现场救治、立体后送为关键环节的现场救援技术体系,即"检、监、救、治、送";以预防预警、损害控制、早期诊断、分级救治、专科康复为救治流...