DELETION AND INACTIVATION OF RETINOBLASTOMA SUSCEPTIBILITY GENE IN PRIMARY RETINOBLASTOMA

The status and expression of Rb gene was detected and analyzed in 19 surgical retinoblastoma specimens using Rb cDNA 3. 8 kb and 0. 9 kb fragment as probe and antibodies specific for synthetic Rb peptide or expressive product of Rb gene expression plasmld. DNA from those tumors had the hemlzygous deletion in 3 cases, the homozygous internal deletion In 2 cases and alterated restriction fragment involving In one copy of Rb gene In 1 case. The quantity of Rb protein demonstrated either absence of reduction in all the 16 cases examined In comparison with that in normal adult retina. It suggested that there were structural or/ and functional defects of Rb gene In retinoblastoma cells and provided evidence to support Knudson' s two hit hypothesis.     

肝癌、癌旁肝组织IGF-Ⅱ、IGF-Ⅱ受体及CSF-1受体/c-fms癌基因产物的表达

本文采用免疫组织化学(ABC)法、Western印迹法和Northern印迹法从细胞、蛋白及转录水平观察17例肝癌及癌旁肝组织中IGF-Ⅱ、IGF-Ⅱ受体和CSF-1受体/c-fms癌基因产物的表达。结果表明,肝癌组织中3种基因产物的表达水平显著高于正常肝组织;癌旁肝组织中IGF-Ⅱ和IGF-Ⅱ受体的表达水平显著高于肝癌组织;肝癌及癌旁肝组织IGF-Ⅱ基因呈胚胎型表达特点。但是,肝癌组织中CSF-1受体基因表达则显著高于癌旁肝组织。由此提示3种基因产物异常的过量表达与肝癌细胞自分泌生长刺激机制有关。     

人巨细胞病毒基因工程糖蛋白52kd抗原的临床初步应用

作者在构建人巨细胞病毒(HCMV)糖蛋白52 kd抗原编码区段的表达质粒pHCMV-52基础上,从含该克隆基因的大肠杆菌中分离并纯化其表达产物作为抗原,与已知ELISA-HCMV-IgM为阳性(12例)和阴性(4例)的血清反应,采用辣根过氧化物酶(HRP)标记的马抗人抗体取代同位素检测特异抗原—抗体复合物,两种方法结果一致者10例(10/12)。该临床初步结果显示,基因工程技术制备HCMV抗原可用于HCMV感染的临床诊断,并具有抗原制备方便、产量高、临床实用和易于推广等优点。