丝光绿蝇抗菌物质在不同pH下抑菌活性的研究

Objective To investigate the antibacterial activity of antibacterial substance in Lucilia seri-cata induced by pricking the skin of maggots(fly s larvae)at different pH conditions. Methods The hemo-lymph from the third instar maggots was collected 48 h after pricking,and the antibacterial effect was tested a-gainst Micrococcus lysodeikticus and Staphylococcus aureus on plates at different pH conditions.The antibacterial effect against M.1ysodeikticus was also detected after the hemolymph、was diluted by different pH buffer solu-tions.The bacteriostatic effects were observed after 24 h and the diameters of clear inhibitory ring were recorded to express antibacterial activity. Results The antibacterial activity of the maggot hemolymph against M.lyso-deiktics and S.aureus was stronger on the plates at pH 6.0 and pH 6.5 than that on the other plates.In the group diluted by pH 6.0 buffer solutions.the antibacterial ring of the maggot hemolymph against M lysodeiktic-us was larger than that in others. Conclusion The results implied that the induced antibacterial substance in Lucilia sericata exhibited stronger antibacterial activity under acidic condition.     

78例妊娠高血压综合征患者血液流变性观察

目的 :观察妊高征患者血液流变学指标的变化。方法 :用北京普利生LBY N6A自清洗旋转式粘度计测定正常孕妇 32例 (对照组 )和妊高征患者 78例 (轻度组 33例、中度组 2 0例、重度组 2 5例 )的全血粘度、血浆粘度、HCT、ESR、血沉方程K值、红细胞聚集指数、红细胞变形指数等血液流变学指标。结果 :与对照组比较 ,轻度组只有红细胞聚集指数升高 (P <0 .0 5 ) ;中度组的HCT、ESR、血沉方程K值、全血低切粘度、红细胞聚集指数均明显升高 (P <0 .0 5～ 0 .0 1 ) ,红细胞变形指数明显下降 (P <0 .0 5 ) ;重度组的HCT、ESR、血沉方程K值、红细胞聚集指数及全血粘度 (低切、中切、高切 )均明显升高(P <0 .0 5～ 0 .0 1 ) ,红细胞变形指数明显下降 (P <0 .0 1 )。结论 :各期妊高征患者血液流变学指标有不同程度的改变 ,重度妊高征改变最显著。因而对妊高征的病情监测和指导治疗有一定的临床价值     

3D 电视胸腔镜肺占位病变切除术

目的：探讨Storz 3D电视胸腔镜手术治疗肺部疾病的可行性。方法2013年7月应用Storz 3D电视胸腔镜分别行肺叶切除联合系统性淋巴结清扫和病变局部切除各2例。选择腋中线第7肋间为胸腔镜观察孔,腋前线第3或第4肋间2～3 cm切口为胸腔镜主操作孔,腋后线靠后第8或9肋间2 cm切口为第1副操作孔。佩戴3D偏振眼镜,在3D效果的手术画面上,行局部病灶切除或肺叶切除,恶性肿瘤者行系统性淋巴结清扫。结果4例均手术成功,无中转开胸,无术后并发症,无死亡。手术时间分别为36、38、72、90 min,出血量分别为5、10、35、80 ml,术后住院时间分别为3、3、5、7 d。术后随访2个月,均无并发症发生。结论 Storz 3D电视胸腔镜手术安全可行,相对于VATS,视野更逼真,操作更灵活稳定。