降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

慢性乙型肝炎患者不同病毒载量与乙肝三系统及前S_1抗原相关性研究

目的研究慢性乙型肝炎患者不同病毒载量与乙肝三系统及前S_1抗原(Pre-S_1Ag)之间的相关性分析。方法血清HBV DNA检测采用荧光定量PCR法(FQ-PCR),乙肝三系统及前S_1抗原(Pre-S_1Ag)检测采用ELISA法;对87例慢性乙型肝炎患者血清进行乙型肝炎病毒载量检测,以乙型肝炎病毒载量为标准,分为A组:(10~3～10~4)拷贝/mL,B组:(10~5～10~6)拷贝/mL,C组:10~7拷贝/mL,检测乙肝三系统及Pre-S_1Ag。结果 A组:(10~3～10~4)拷贝/mL,检出率最高的模式为小三阳、Pre-S_1Ag(一);B组:(10~5～10~6)拷贝/mL,检出率最高的模式为小三阳、PreS_1Ag(+);C组:10~7拷贝/mL,检出率最高的模式为大三阳、Pre-S_1Ag(+)。结论乙肝三系统、前S_1抗原与HBV DNA定量水平之间具有良好的相关性,能敏感地反映乙型肝炎病毒复制情况。     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.     

降钙素基因相关肽、内皮素与2型糖尿病肾病的相关性研究

Objective To investigate the tendency of plasma levels of calcitonin gene-related peptide(CGRP)and endothelin(ET)in type 2 diabetic nephropathy,and explore the relationship between calcitonin gene-related peptide,endothelin and pathogenesis of diabetic nephropathy.Methods A total of 25 healthy controls(healthy control group)and 83 patients with type 2 diabetic mellitus(T2DM group)were enrolled in the investigation.According to level s of urinary albumin excretion rate(UAER),the 83 T2DM patients group were further divided into three subgroups:normal UAER subgroup(n=28),microalbuminuira subgroup(n=29)and clinical proteinuria subgroup(n=26).Plasma contents of calcitonin gene-related peptide and endothelin were measured with radioimmunoassay(RIA).Results The level of ET was significantly higher in T2DM group than that in healthy control group(P＜0.01),positively correlative tO UAER(r=0.65,P＜0.01).The plasma level of CGRP was significantly lower in the patients with diabetic nephropathy than the healthy controls,negatively correlative tO UAER(r=-0.71,P＜0.01).There was negative correlation between plasma levels of ET and CGRP(r=-0.62,P＜0.01).Conclusion The disequilibrium of CGRP and ET in plasma might play an important role in the process of diabetic nephropathy.Therefore,keeping balance of levels of CGRP and ET in plasma contributes to prevention and cure of diabetic nephropathy.