闭合穿刺克氏针、钢丝绞锁内固定治疗髌骨骨折12例

自 1 991年 1 0月至 2 0 0 0年 6月共治疗髌骨骨折 31例 ,其中 1 2例采用闭合穿刺克氏针加钢丝绞锁内固定术 ,效果满意 ,现报告如下。1　临床资料1 .1　一般资料　男 1 0例 ,女 2例。1 9～ 30岁 4例 ,31～40岁 7例 ,5 0岁 1例。横断型骨折 9例 ,下极骨折 2例 ,上极骨折 1例 ,所有病例均在伤后一天内入院。骨折端分离 1～ 4cm,病例选择为非粉碎性骨折 ,无严重并存疾病。1 .2　手术方法　 1用 2 %普鲁卡因 5～ 1 0 ml,局部浸润麻醉。普鲁卡因过敏者改用 1 %的利多卡因浸润麻醉。2髌韧带和股四头肌腱膜都十分坚固 ,故髌骨骨折后 ,位置较衡定。…     

断指再植术后手指功能的再训练

目的 介绍49例断指再植术后的康复护理方法及效果。方法 对再植指进行物理疗法、中药薰洗、被动和主动的运动训练、感觉训练等。结果 49例中优良率达93.9%，恢复原工作率达87％。结论 断指再植术后同期进行系统的康复护理，是加速和最大限度恢复患指功能的有效方法。     

Differentiation of Helicobacter pylori isolates by polymerase chain reaction—restriction fragment length polymorphism

Objective: To investigate the association between the diversity of urease gene and urease activity of clinical isolates of Helicobacter pylori (H. pylori). Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of urease gene and rapid urease activity test were used to study the urease activity of different clinical isolates of H. pylori. Results: H. pylori clinical isolates were divided into 4 types according to their PCR-RFLP results of urease gene and urease activity. Type Ⅰ , possessing strong urease activity (0. 11) and presented 1 fragment of 1. 7 kb by PCR-RFLP, had close relations with gastric ulcer; type Ⅱ , with the weakest urease activity (0. 07) and 2 fragments (1. 3 and 0. 4 kb respectively) , was associated with duodenal bulb ulcer; type Ⅲ , with the strongest urease activity (0. 12) and 2 fragments (0. 4 and 0. 17 kb) with or without 1 fragment (0. 23 or 0. 37 kb) , was responsible for gastritis; type Ⅳ , with weak urease activity (0. 09) and 2 fragments     

钙粘附蛋白在子宫内膜及蜕膜的表达

目的：分析钙粘附蛋白（cadherin)在月经周期各阶段子宫内膜和蜕膜中的表达及其免疫组化染色特点。方法：应用特异性单克隆抗体，SABC免疫组化技术，采用微波缓冲液抗原修复和复合消化液修复法，检测45例月经周期不同阶段的子宫及早期妊娠蜕膜中上皮型钙粘附蛋白（E－cadherin)的表达。结果：子宫内膜腺上皮各阶段均见E－cadherin的表达，但在植入窗期表达最高，分泌晚期及早孕蜕膜表达降低。间质细胞仅在早孕蜕膜有少量表达。微波修复法阳性率明显较复合消化液法高。结论：钙粘附蛋白在子宫内膜植入窗期表达增高可能与胚泡粘附及植入过程有重要关系。检测该抗原宜采用微波抗原修复法。