排序方式: 共有38条查询结果,搜索用时 15 毫秒
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选择广州医科大学附属第一医院2018年3—8月因急性加重住院的慢性阻塞性肺疾病(慢阻肺)患者48例, 按照入院顺序随机分为改进用药组(A)、常规用药组(B)和非用药组(C), 分别为18、15和15例。针对性改进患者使用噻托溴铵能倍乐的吸入方法:身体前倾, 缩胸彻底呼气后, 扩胸挺胸深长吸气。观察患者6 min步行距离。第1天3组基础6 min步行距离分别为(315±80)、(288±122)和(251±89)m;第2天A、B两组采用常规方法吸入噻托溴铵能倍乐, 6 min步行距离分别增加至(358±76)和(322±120)m;第3天, A组采用改进方法吸入后, 进一步增加至(406±70)m, B组继续采用常规方法吸入后仅增加至(331±121)m;非用药组3 d的6 min步行距离没有显著改变;A、B组采用说明书常规吸入方法的吸入持续时间为(2.8±0.5)s, 采用改进吸入方法的吸入持续时间显著延长至(5.2±1.3)s(F=4.18, P=0.04);患者对改进吸入方法的学习能力跟学历呈正相关、与年龄呈负相关。相比常规吸入方法, 身体前倾缩胸、彻底呼气后扩胸挺胸深长吸气法吸入... 相似文献
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母乳喂养是最科学的喂养方式,是能够保证婴儿健康的喂养方式。许多学者认为,婴幼儿龋与喂养方式有关。动物实验研究显示母乳的致龋性甚至高于牛乳,但此观点仍缺乏流行病学的依据。也有人指出母乳喂养及其持续时间与婴幼儿龋无关。现将国内母乳喂养影响儿童牙齿健康文献综述如下: 相似文献
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目的 观察脑内移植成体神经干细胞对脑外伤大鼠中枢神经功能的影响.方法 将60只Wistar大鼠随机分为5组,正常组(A)、模型组(B)、假手术组(C)、PBS移植组(D)、干细胞移植组(E).Morris水迷宫实验测试各组大鼠的学习记忆能力;免疫组织化学及酶联免疫吸附试验(ELISA)检测大鼠脑组织中脑源性神经生长因子(BDNF)、神经生长因子(NGF)的表达.结果与A组比较,B组大鼠寻找隐形平台的时间明显延长(P<0.01),脑组织中BDNF(27.70±1.65)ng/g蛋白、NGF(59.80±9.40)ng/g蛋白含量下降显著(P<0.01),模型复制效果理想;与B组比较,E组大鼠学习及功能明显改善,脑内BDNF(49.30±2.87)ng/g蛋白、NGF(88.50±9.23)ng/g蛋白的表达量上升,差异有统计学意义(P<0.01).结论 成体神经干细胞的脑内移植能明显恢复外伤大鼠中枢神经功能.Abstract: Objective To study the neuroprotetive effect of dault neural stem cells intracerebral implantation to rats subjected to traumatic brain injury.Methods Sixty Wistar rats were divided into 5 groups randomly: normal group ( A ),model group ( B),sham-operated group ( C ),PBS transplated group (D),neural stem cells transplated group (E).Morris maze method was adopted to observe the impact on the learning and memory.Immunohistochemisty and enzyme linked immunosorbent assay (ELISA)were used to detect the expression of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in brain issue.Results As compared with group A,in Morris maze experiment,time to reach the end point of the maze in group B was significantly prolonged ( P < 0.01 ),and the content of BDNF (27.70 ± 1.65 ) ng/g prot and NGF ( 59.80 ± 9.40 ) ng/g prot were decreased significantly ( P < 0.01 ).As compared with group B,the function of learning and memory was obviously improved,and the expression of BDNF (49.30 ±2.87) ng/g prot and NGF (88.50 ±9.23) ng/gprot in brain issues were statistically increased in group E (P <0.01 ).Conclusion Transplantation of adult neural stem cells can ameliorate learning and memory deficits following traumatic brain injury in rats. 相似文献
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目的 探讨体外建立培养成体神经干细胞(MSC)的方法.方法 从6周龄大鼠脑组织中分离神经干细胞,用神经干细胞培养液[DMEM/F12培养液添加1%N2、2%B27、20 μg/L表皮生长因子(EGF)和20μg/L碱性成纤维细胞生长因子(bFGF)]使其稳定增殖,10%胎牛血清诱导其贴壁分化.倒置显微镜下观察神经干细胞形态学变化;流式细胞仪检测神经干细胞表面标记物巢蛋白(Nestin)、神经元特异性烯醇酶(NSE,成熟神经元的特异性标志)、半乳糖脑苷脂(Galc-C,成熟少突胶质细胞的标记物)的表达.结果 分离所得细胞能在体外传代培养,流式细胞仪检测显示Nestin阳性细胞为97.6%,细胞经胎牛血清诱导分化后能形成NSE、Galc-C阳性细胞.结论 采用无血清的神经干细胞培养液能培养出具有分化潜能的成体神经干细胞. 相似文献
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教学是在教育目的规范下,教师的教与学生的学共同组成的双边活动 是严密组织起来的传授系统科学文化知识,促进学生发展的最有效的形式,是实现学校培养目标的最基本途径。因此,教学在学校工作中居于十分重要的地位。如何提高教学的有效性已成为各类学校的中心问题,本文力图从学校教育情境中师生关系方面来谈谈加强教学的有效性,从而提高教学质量。 相似文献
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To obtain single chain variable fragment (scFv) and bivalent single chain variable fragment (bsFv) against transferrin receptor, up-stream and down-stream primers were designed according to the complementary sequences of FR1 region of variable heavy (VH) and FR4 of variable light (VL), respectively, which contained inter-linker G4S and the restriction endonuclease SfiI, AscI and NotI. Two pieces of scFv fragments were first amplified through PCR and then inserted into plasmid pAB1, which could express scFv protein once induced by IPTG in the host bacteria. To express scFv and bsFv, E. coli TG1 was cultured in LB broth and was induced by IPTG. The restriction enzyme digestion map and DNA sequencing demonstrated that scFv and bsFv genes were successfully inserted into the expression plasmid. SDS-PAGE and Western blotting revealed the protein band at 35kD and 60kD, which were consistent with the molecular weight of scFv and bsFv respectively. Flow cytometry showed that scFv and bsFv harbored the specific binding activity with TfR expressed in various tumor cells, and the avidity of bsFv was higher than that of the parent scFv. 相似文献
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目的 研究过长茎突组织标本的病理学特点。方法 对21例茎突切除标本行组织学观察,其中17例临床诊断为茎突过长综合征,茎突长度34~68 mm,平均49.6 mm,中位长度56 mm;4例为腮腺区恶性肿瘤累及茎突,茎突长度30~40 mm。结果 13例茎突过长综合征患者的茎突为成熟板状骨,4例为管状骨内含红骨髓,其中3例呈现软骨内成骨,1例同时呈现软骨内成骨和膜内成骨;4例恶性肿瘤侵蚀的茎突表现为骨质破坏,未见明显成骨。结论 部分茎突过长组织呈现出活跃的成骨特点,提示对于这部分患者手术阻断茎突的生长可能更为必要,恶性肿瘤累及的茎突未见明显成骨现象。 相似文献