腰椎管狭窄症50例临床分析

<正> 早在1936年 Towne 等首次对该病2例进行描术,1954Verbiest 提出椎管狭窄症。嗣后对此病陆续有报道.现将1982～1988年我科收治的50例手术病人进行总结.临床资料男38例,女19例,年龄26～65岁,全部进行手术治疗。发病至于术时间7个月～30年,平均6年1个月.     

Analysis of differentially expressed genes in fetal skin of scarless and scar-forming periods of gestational rats

Objective: To study the differences of gene expression between earlier gestational skin and later gestational skin of rats with the aids of single primer amplification (SPA) and high-density oligonucleotide DNA array to understand the molecular mechanism of scarless healing. Methods: Total RNAs were isolated from fetal rat skin of the scarless (E15) and scar-forming (E18) periods of gestation (term =21.5 days). The RNAs from earlier gestational skin (EGS) and later gestational skin (LGS) were both reversely transcribed to cDNAs, then labeled with the incorporation of fluorescent dCTP for preparing the hybridization probes by SPA method. The mixed probes were then hybridized to the oligonucleotide DNA arrays which contained 5 705 probes representing 5 705 rat genes. After highly stringent washing, these DNA arrays were scanned for fluorescent signals to display the differentially expressed genes between the 2 groups of skin. Results: Among 5 705 rat genes, there were 53 genes (0.93%) with differentially expressed levels between EGS and LGS groups, 27 genes, including fibroblast growth factor 2 ( FGF2 ) and follistatin were up-regulated (0.47%) and 26 genes were down-regulated (0.46%) in fetal skin during scarless period versus scar-forming period. Higher expressions of FGF2 and follistatin in EGS than those in LGS were also revealed by RT-PCR method. Conclusions: High-density oligonucleotide DNA array provided a powerful tool for investigating differential gene expression in earlier and later gestational fetal skins. This technology validates that the mechanism of fetal scarless healing is very complicate and the change of many gene expressions is associated with fetal scarless healing.     

冷眼看待舆论救援“1元药”

10月3日,《新闻晨报》载文称,"沪产的传统经典药如痱子粉、足光粉、开塞露为何越来越难觅踪影?最近,沪上不少市民又在为购买便宜的经典药而奔波于各大药房。记者调查后发现,有的药已改头换面以昂贵的身价露面;还有一些药因为价格太低廉,低利润令药厂、药房都纷纷‘敬而远之’,跑十几家药房没买到便宜药‘,1元药’最易消失。"     

幽门螺杆菌,维生素C和胃癌关系的研究进展

幽门螺杆菌、维生素Ｃ和胃癌关系的研究进展龙允穗综述陈振侬、洪瑞香审校广西医科大学内二科南宁５３００２２幽门螺杆菌（ＨｅｌｉｃｏｂａｃｔｅｒＰｙｌｏｒｉＨＰ）感染与慢性胃炎（Ｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓ，ＣＧ），消化性溃疡（Ｐｅｐｔｉｃｕｌｃｅｒ，...     

异基因骨髓移植后供者干细胞输注与供者淋巴细胞输注抗白血病作用的比较

异基因骨髓移植后白血病复发是一个重要的问题,其发生率达10%～36%.在疾病晚期和采用去T细胞移植的患者中复发率可高达70%以上[1],对于此类患者联合化疗效果差,而二次移植则只在晚期复发的患者中效果较好[2,3].一些研究者曾采用供者免疫活性细胞输注或使用人重组白细胞介素2来刺激供者造血或增强移植物抗白血病作用(graft-versus-leukemia,GVL)[4-6].我们报告了20例异基因骨髓移植后复发的白血病患者,对他们分别进行了无粒细胞集落刺激因子(G-CSF)动员的供者淋巴细胞(donor-derived lymphocyte,DDL)输注或G-CSF刺激后采集的供者干细胞(peripheral blood progenitor cell,PBPC)输注.     

16种抗生素在两种培养基上抑菌效果观察

细菌学纸片扩散法药物敏感试验的标准为Kirby-Bauer法，该法指定选用Mueller－Hinton琼脂（MHA）作为实验用培养基。为了解抗生素在普通营养琼脂（NA）和MHA培养基上抑菌效果的差异，我们选择了16种常用抗生素，对照观察了各种抗生素对某一菌株在这两种培养基上抑菌圈直径的差别。回材料和方法1．1材料氨于西林、校等西林、听拉西林、头抱哩琳、头抱拉定、头抱磷脂、头抱曲松、头抱响酮、头抱他定、庆大霉素、受市霉素、链霉素、阿米卡星、诺氟沙星、环丙沙星和氧氟沙星共16种。NA、MHA和药敏纸片，均采用杭州康壮生物工程公司产品。…     

久留米大学及其医学院的概况与特点

久留米大学及其医学院的概况与特点中国医科大学孙贺一路振富久留米大学位于日本西南部的福冈县久留米市，其环境幽美，山清水秀，面临广阔的“筑后”平原。该大学创建于１９２８年，是在九州医学专科学校的基础上扩大发展起来的一所私立综合大学。一、一般情况久留米大学...