血浆同型半胱氨酸水平与脑卒中的关系研究

目的探讨血浆同型半胱氨酸水平与脑卒中的关系。方法采用全自动生化分析仪,以小分子捕获技术循环酶法对127例脑卒中患者（75例脑梗死患者,52例脑出血患者）Hcy水平进行检测,并与健康体检者进行对照,应用统计学方法进行数据分析。结果全部脑卒中患者血浆Hcy水平均高于健康对照组（P〈0．01）,而脑梗死组和脑出血组差异无统计学意义（P〉0．05）。结论血浆Hcy水平升高与脑卒中有密切关系。     

心脏型脂肪酸结合蛋白对急性心肌梗死早期诊断的临床价值研究

Objective To study the clinical value of heart-type fatty acid-binding protein (H-FABP) in early diagnosis of acute myocardial infarction(AMI). Methods Totally 156 patients who were suspicious to be AMI were divided into group A (0～3 h group) and group B (3～6 h group) ac-cording to the visit time. H-FABP,cardiae troponin T (cTnT) and cardiac troponin T (cTnT);Crea-fine kinase isoenzyme-MB (CK-MB) were simultaneously measured at different time-points. The sensi-tivity,specificity and accuracy of the markers were calculated and compared. Results The sensitivity, specificity and accuracy of H-FABP were all the highest among three tumor markers;the sensitivity within 3 hours was 70.7% ,higher than that of cTnT and CK-MB remarkably (P<0.05);the specific-ity was 97.0%; the accuracy was 82.4%, higher than that of cTnT and CK-MB remarkably (P<0.05);while the sensitivity within 3～6 hours was 100.0%, higher than that of cTnT remarkably (P <0.05);the specificity was 92.6%;and the accuracy was 97.6%. Conclusion H-FABP is the most sensitive indicator for early diagnosis of AMI and is of very important value in clinical application.     

Rapid Identification of Pathogenic Bacteria by means of TwoConservative Gene Loci′ Specific PCR-CE-RFLP

Molecular diagnosis is playing an increasingly importantrole in the rapid detection and identification of pathogenicorganisms in clinical samples. The genetic variation of ri bosomal genes in bacteria offers an alternative chance forthe detection and identification of these organisms. TherRNA genetic locus, rrn, is found in all prokaryotic or ganisms, and is highly conservative, although its relativelystable variation is found frequently in different bacteria.The utility of this lo…     

慢性肾病患者血1,25-二羟基维生素D3水平变化

目的 研究慢性肾病(CKD)患者血1,25-二羟基维生素D3(维生素D3)水平异常与肾功能受累程度的关系.方法 研究对象包括150例健康对照者和101例CKD患者,检测其血维生素D3、甲状旁腺素、钙、磷、肌酐、清蛋白及尿素氮水平,计算肾小球滤过率(GFR).结果 与对照组相比,第4~5期患者的血维生素D3水平显著降低(P<0.05),第3~5期患者的血甲状旁腺素(PTH)、肌酐及尿素氮(BUN)水平显著升高(P<0.05),第4~5期患者的血磷水平显著升高(P<0.05),各期CKD患者的清蛋白水平均显著降低(P<0.05),仅第5期CKD患者的血钙浓度显著降低(P<0.05).血维生素D3水平与血PTH、尿素氮及肌酐水平呈负线性相关关系,与血钙、清蛋白及GFR水平呈正线性相关关系.结论 第4~5期CKD患者缺乏维生素D3,说明只有当肾功能受损严重时,CKD患者血维生素D3水平才会出现明显异常.此外,本研究还表明CKD患者血维生素D3水平与其他肾功能相关生化指标存在一定的相关性.     

CK-MB活力高于CK活力的异常结果探讨

ＣＫ－ＭＢ活力高于ＣＫ活力的异常结果探讨大连市临床检验中心（１１６０３１）温杰刘淑琴大连市中心医院（１１６０３１）徐维家杜斌在近两年里，我们在实验室心肌酶的测定中，人体组织细胞质中的肌酸激酶（ＣＫ）由Ｍ亚型和Ｂ亚型组成。以三种同功酶形式存在即脑型ＣＫ...     

急性冠脉综合征患者血清基质金属蛋白酶-9与超敏C反应蛋白检测的临床意义

目的研究急性冠脉综合征(ACS)患者血清中的基质金属蛋白酶-9(MMP-9)和超敏C反应蛋白(hs-CRP)的水平变化,探讨其在ACS诊断和预测中的临床价值。方法选取临床确诊的ACS患者53例,其中急性心肌梗死(AMI)组24例,不稳定型心绞痛(UA)组29例,以同期、同年龄健康体检者30例作为对照。分别采用酶联免疫吸附法(ELISA)检测血清MMP-9浓度和免疫透射比浊法检测血清hs-CRP浓度。结果 AMI组和UA组患者血清MMP-9和hs-CRP浓度均明显高于对照组,差异有统计学意义(P<0.01),且AMI组患者与UA组患者间比较也存在统计学差异(P<0.01),AMI组患者在发病初期与治疗后血清MMP-9和hs-CRP水平比较也存在统计学差异(P<0.01)。结论 ACS患者血清MMP-9、hs-CRP水平是2个有价值的冠脉损伤的指标,可作为冠脉事件预测的重要参数。     

组织多肽特异性抗原和癌抗原12-5联合检测卵巢癌的临床应用价值

卵巢癌死亡率居妇科肿瘤之首,且起病隐匿,易早期播散和转移,60%～70%的卵巢癌患者初诊时已是中晚期,该病对化疗敏感,但极易复发.早期发现、及时诊断对有效治疗卵巢癌显得尤为重要.     

1992～2001年10年间沙门菌感染的临床检验与分析

自1986年英国首先报道由肠炎沙门菌(salmonella enteritidis，SE)引起的食物中毒以来，世界范围内沙门菌引起的感染越来越多见。日本西山政孝等将1992～2001年间从住院患者中分离而来的210株沙门菌进行回顾性研究发现，日本沙门菌感染的高发年份自1996年开始出现，1999年达最高峰，为51株，至2000年下降到16株。所有分离株中61．9％为血清型O9群，其次为O4群，占17．1％。分离高峰出现在每年的7～10月间，占总分离数的67．1％(140/210)。