回肠膀胱术后储尿囊类癌一例

患者女性,55岁,因"血尿、腰痛3个月"于2008年11月11日人我院.既往50年前因"膀胱肿瘤"行膀胱全切、回肠代膀胱术,具体病理资料无法考证.3个月前出现淡红色血尿,伴少许血块,有大量的脓白色分泌物随尿液流出,双侧腰部酸胀疼痛明显.体检:右侧麦氏点区域可见回肠膀胱瘘口.尿常规:红细胞(2+),尿白细胞(3+),尿培养为奇异变形菌.血常规:血红蛋白85 g/L,白细胞数11.3×10~9/L.     

多重核酸检测系统同时检测22种呼吸道病原体的应用效果研究

目的 采用多重核酸检测系统同时检测22种呼吸道病原体,了解韶关市儿童急性呼吸道感染(ARI)常见病毒病原体的构成情况及流行特征。方法 选择粤北人民医院作为监测点,按要求及时采集呼吸道患者标本886份送至韶关市疾病预防控制中心微生物检验室,采用多重核酸检测系统定性检测22种呼吸道病原体,包括新型冠状病毒、冠状病毒HKU1、冠状病毒NL63、冠状病毒229E、冠状病毒OC43、人类偏肺病毒、人鼻病毒/肠道病毒、甲型流感病毒、甲型流感病毒H1、甲型流感病毒H1-2009、甲型流感病毒H3、乙型流感病毒、副流感病毒1型、副流感病毒2型、副流感病毒3型、副流感病毒4型、呼吸道合胞病毒、副百日咳杆菌、百日咳杆菌、肺炎衣原体、肺炎支原体。同时采用传统的荧光定量逆转录-聚合酶链反应(RT-PCR)对上述样品进行同样项目的检测,对比检测结果准确性。结果 886份标本中检出阳性425份,核酸检出率为47.97%(425/886),FluA检出率最高,为15.46%,其他病毒的检出率从高到低依次为鼻病毒(HRV)9.48%、人副流感病毒(HPIV)6.55%、乙型流感病毒(FluB)5.87%、RSV 4....     

基于酶促恒温扩增技术快速鉴别天麻真伪检测方法的建立与应用

目的:基于酶促恒温扩增(enzymatic recombinase amplification, ERA)技术建立一种可快速定性鉴别道地药材天麻真伪的方法。方法:遵循ERA引物设计原则,应用Oligo 7.0软件根据天麻及其常见伪品的ITS2基因组序列,筛选优化天麻ERA特异性引物,应用Primer Premier 5.0软件设计天麻特异性PCR鉴别引物,通过对ERA、PCR反应体系的优化,最终确定ERA最佳反应时间为17 min,最适反应温度为40℃;PCR最优退火温度为57℃,循环为32次,对所建立的方法进行灵敏度、特异性验证,并选取中药市场市售天麻样品进行检测。结果:所设计的天麻ERA鉴别引物与其常见伪品间无交叉反应,特异性良好;重复性结果显示,3次重复性检测结果一致,未出现假阳性与假阴性;该方法对天麻基因组DNA的灵敏度为1 pg·μL^-1,比传统PCR灵敏性高;ERA技术能够用于天麻市售样品的快速鉴定,且检测结果与PCR方法相同。结论:所建立的检测方法操作简单、快速,具有较高的特异性和灵敏度,为道地药材天麻的真伪鉴别提供了一种新手段。     

肾移植术后并发自体尿路上皮多器官癌六例的临床分析

Objective To analyze the etiology, clinical diagnosis and outcomes of 6 cases of multifocal urothelial carcinomas following transplantation. Methods A retrospective analysis was performed on 6 cases of multifocal urothelial carcinomas following renal transplantation in our center. Results Six patients were diagnosed as having multifocal urothelial carcinomas, including 1 case of related renal transplantation. Five cases were diagnosed by painless gross hematuria 2～48 months after renal transplantation, and I patient was diagnosed as having bladder tumors by B-uhrasound. All lesions happened un-simultaneously, from 1.5～16 months, and each case accepted operation 2～5 times. One case accepted radical cystectomy and cutaneous ureterostomy of the graft, and one accepted radical cystectomy, cutaneous ureterostomy of the graft and urethrectomy. All of 6 cases received OKT3 or Daclizumab as the induced-immunosuppressive therapy and tacrolimus or cyclosporine A + mycofenolate mofetil + steroid as the maintenance therapy. Intravesical chemotherapy started in all patients immediately after the surgery. All operations were successful and most patients got satisfactory results. Conclusions Kidney recipients have a higher rate of transitional cell carcinomas which are characterized by easy metastasis, and mostly have unsatisfactory prognosis. Kidney recipients with hematuria should be examined carefully with cystoscopy, retrograde pyelography and cystourethroscopy. A routine examination at regular intervals after transplantation is very important.     

微创经皮肾镜碎石术治疗大于10mm肾下盏结石35例临床分析

目的 探讨B超引导下微创经皮肾镜碎石术治疗直径＞10 mm肾下盏结石的临床效果.方法 回顾分析2007年3月至2011年10月B超引导下行微创经皮肾镜碎石术治疗肾下盏结石35例的临床资料,结石直径11 ～33 mm,按结石直径大小分为两组.10 ～20 mm组15例,＞20 mm组20例,F16～F20单通道取石.结果 35例均成功,手术时间30.0～130.0 min,平均(75.4±20.2)min;Ⅰ期碎石31例,Ⅱ期碎石4例;18例为肾下盏通道,12例为肾中盏通道,5例为肾上盏通道;术后腹部X线平片发现3例结石残留,10～20 mm组和＞20mm组B超引导下微创经皮肾镜碎石术无石率分别为93.3％(14/15)和90.0％ (18/20),两组差异无统计学意义(P=0.90).总体无石率为91.4％ (32/35).术后输血2例,高热3例.结论 B超引导下微创经皮肾镜碎石术具有成功率高、安全、创伤小等特点,是治疗直径＞10 mm肾下盏结石的合适选择.     

人类精浆中多胺的氧化与免疫性不育的关系

人类精浆在体内和体外均有一种潜在的免疫抑制作用,这种作用有助于减少免疫致敏作用,保护精子免受免疫性损害。Rees等的研究支持了多胺氧化在精浆免疫抑制中起重要作用的观点。     

肾移植术后并发自体尿路上皮多器官癌六例的临床分析

Objective To analyze the etiology, clinical diagnosis and outcomes of 6 cases of multifocal urothelial carcinomas following transplantation. Methods A retrospective analysis was performed on 6 cases of multifocal urothelial carcinomas following renal transplantation in our center. Results Six patients were diagnosed as having multifocal urothelial carcinomas, including 1 case of related renal transplantation. Five cases were diagnosed by painless gross hematuria 2～48 months after renal transplantation, and I patient was diagnosed as having bladder tumors by B-uhrasound. All lesions happened un-simultaneously, from 1.5～16 months, and each case accepted operation 2～5 times. One case accepted radical cystectomy and cutaneous ureterostomy of the graft, and one accepted radical cystectomy, cutaneous ureterostomy of the graft and urethrectomy. All of 6 cases received OKT3 or Daclizumab as the induced-immunosuppressive therapy and tacrolimus or cyclosporine A + mycofenolate mofetil + steroid as the maintenance therapy. Intravesical chemotherapy started in all patients immediately after the surgery. All operations were successful and most patients got satisfactory results. Conclusions Kidney recipients have a higher rate of transitional cell carcinomas which are characterized by easy metastasis, and mostly have unsatisfactory prognosis. Kidney recipients with hematuria should be examined carefully with cystoscopy, retrograde pyelography and cystourethroscopy. A routine examination at regular intervals after transplantation is very important.     

精囊囊肿1例报告

患者男，48岁，已婚已育。间断左下腹痛20a，加重伴全程肉眼血尿6d入院。患者无尿频、尿急、尿痛、血精及排尿困难。直肠指诊：前列腺左上方饱满，囊性感。腹部B超检查：前列腺左后方可见90mm×18mm大小条索状及52mm×41mm大小类圆形囊性包块，似相连通；左肾区未见正常肾脏影像。经直肠B超检查见左侧精囊区65mm×51mm大小囊性包块，边界清，不规则，内呈多发、分隔状。阴囊B超检查见左侧附睾尾部增宽，内呈多发、小囊样扩张。CT见前列腺左后方可见不规则分叶状肿块影，与肠管分界不清，其内密度均匀，CT值51HU。诊断：精囊囊肿，左肾缺如。在硬膜外麻醉下行剖腹探查术。于膀胱后方见左侧精囊呈多房性囊状增大，     

肾移植术后并发自体尿路上皮多器官癌六例的临床分析

Objective To analyze the etiology, clinical diagnosis and outcomes of 6 cases of multifocal urothelial carcinomas following transplantation. Methods A retrospective analysis was performed on 6 cases of multifocal urothelial carcinomas following renal transplantation in our center. Results Six patients were diagnosed as having multifocal urothelial carcinomas, including 1 case of related renal transplantation. Five cases were diagnosed by painless gross hematuria 2～48 months after renal transplantation, and I patient was diagnosed as having bladder tumors by B-uhrasound. All lesions happened un-simultaneously, from 1.5～16 months, and each case accepted operation 2～5 times. One case accepted radical cystectomy and cutaneous ureterostomy of the graft, and one accepted radical cystectomy, cutaneous ureterostomy of the graft and urethrectomy. All of 6 cases received OKT3 or Daclizumab as the induced-immunosuppressive therapy and tacrolimus or cyclosporine A + mycofenolate mofetil + steroid as the maintenance therapy. Intravesical chemotherapy started in all patients immediately after the surgery. All operations were successful and most patients got satisfactory results. Conclusions Kidney recipients have a higher rate of transitional cell carcinomas which are characterized by easy metastasis, and mostly have unsatisfactory prognosis. Kidney recipients with hematuria should be examined carefully with cystoscopy, retrograde pyelography and cystourethroscopy. A routine examination at regular intervals after transplantation is very important.