氮烯乙茶治疗晚期恶性组织细胞病

氮烯乙茶(7—乙基—8二甲基三氮烯茶碱,EDMTP)为一种抗癌活性较强的新型化合物,对小鼠移植性L_(615)白血病有治愈活性。对某些实体瘤也有很好的抑瘤效应。我院应用该药对2例晚期恶性组织细胞病(简称恶组)治疗后短期内获完全缓解(其中1例已存活5年)。报告如下。     

肝移植术后并发症初探

目前 ,肝移植已经成为治疗终末期肝病的有效治疗方法。我院从 2 0 0 0年 4月起采用经典式原位肝移植治疗晚期肝硬化和肝脏肿瘤 ,取得较好疗效。现将手术中出现的一些并发症进行讨论。1.资料与方法 :本组病人共 5例 ,全部为男性。年龄 38～ 5 6岁。其中晚期肝硬化 1例 ,肝癌伴肝硬化 3例 ,肝门胆管癌 1例。所有病人均采用全麻下经典式原位肝移植术 ,术后置Ｔ管引流。术后观测病人体温、胆汁引流量 ,同时行动态腹部Ｂ超检查 ,必要时行ＣＴ和经皮肝脏穿刺活检检查。2 .结果 :5例病人无围手术期死亡 ,术中输血2 0 0 0～ 6 0 0 0ｍｌ。病人术后出…     

《本草品汇精要》草部七种药物的考证

1 概述《本草品汇精要》以宋《证类》为基础,对北宋至金元、明中叶约三百余年间药物的发展与变迁及对旧本草药物品种的增补与改移作了一次较系统的总结.虽然该书大部分品种承《政和本草》,对此后的本草如《绍兴本草》、《饮膳正要》以及金元医方本草中的品种亦注意收集,汇其品种,述其精要.因此,考察该书的药用品种,对研究当时用药的基源有十分重要的意义,对今天澄清药用品种的混乱历史亦提供了线索。全书植物类药物(包括草、木、果、菜、米谷诸部)粗略统计约1150种,今国内现存部分残卷中植物类药物除去重复者计400余种,药图600余幅,其中新     

医绩卓著 誉满陇上 承前启后 丰功永存——张汉祥生平及学术思想介绍

张汉祥(1911～1983年),天津市人,甘肃当代著名中医。他毕生致力于继承和发展祖国的医学事业,在近半个世纪的医疗实践中,医绩卓著,誉满陇原,深受甘肃人民的信赖和崇敬。1935年张老拜北平名医方伯屏先生为师,学习中医,他以强烈的求知欲望,至诚的学习决心,勤求古训,博采众方,耽嗜典籍,如啖蔗饴,终于达到通篇背诵《伤寒     

化石汤对泌尿系结石影响的扫描电镜观察

中医治疗泌尿系结石已积累了丰富的经验。七十年代时我国开展中西医结合疗法治疗本征,排石率达68％。有报导用中西医结合疗法治疗泌尿系结石,可碎石和溶石,但上述报道,多都根据肉眼及X线照片观察,用扫描电镜(SEM)观察,则尚未见报导。现将我们对3例结石的观察结果,报告于后,并作简要的讨论。材料与方法我院泌尿结石中医专科门诊,五年来共治疗病人152人。排石率为72.5％。本材料选自经治愈的典型病例3例,观察了此3例患者服药后排出之结石标本共4粒。按扫描电镜标本制备法,将标本水洗后,自然干燥,用     

体外诱导骨髓间充质干细胞向肾小管上皮样细胞分化

Objective To investigate the differentiation of rat bone marrow mesenchymal stem cells (MSCs) to renal tubular epithelial-like cells under different conditions. Methods MSCs were obtained from rat marrow. MSCs were isolated by gradient density centrifugation and plastic adherence and then purified. Surface markers were identified with flow cytometry after amplification in vitro. The purified MSCs of the third passage were cultured respectively as follows: (1) control group: DMEM medium with fetal bovine serum(FBS). (2) all-trans retinoic acid (ATRA) group: DMEM medium with FBS, ATRA and ischemic reperfusion-injured kidney tissue homogenate. (3)combination group: DMEM medium with FBS, ATRA, ischemic reperfusion-injured kidney tissue homogenate, epidermal growth factor (EGF) and bone morphogenetic protein 7 (BMP-7). After 7 days, the MSCs were collected for alkaline phosphatase (AKP) staining, cytokeratin-18 and E-cadherin immunocytochemical analysis. Results The positive rates of the third passage MSCs in CD44, CD90 and CD29 were 97.8%±0.9%, 96.8%±1.4% and 97.6%±2.4%,respectively, but in CD11b/c and CD34 were only 13.2%±0.6% and 1.2%±0.5%. The MSCs in control group were spindle. The MSCs in ATRA group were round and elliptic. The MSCs in combination group became cobblestone-like cells after 7 days. AKP staining showed that tubular epithelial-like cells from MSCs in control group were negative, some above cells in ATRA group were positive and number of above cells increased in combination group. Compared with negative control group, the ratios of cytokeratin-18 positive cells in ATRA group and combination group were respectively increassed by 29.47%±1.08% and 47.52%±2.13% (all P<0.05), the ratios of E-cadherin positive cells in ATRA group and combination group were respectively increased by 14.88%±2.46% and 36.15%±1.13% (all P<0.05). Conclusion MSCs may differentiate by renal tubular epithelial-like cells under the induction of ischemic reperfusion-injured kidney tissue homogenate and ATRA in vitro, which are further differentiated under the combined induction of EGF and BMP-7.