贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

Objective To investigate plasma glutathione S-transferase(GSTs) activity and GSTP1 gene Ile105Val polymorphism in Bijie City, Guizhou Province, a coal-burning fluorosis endemic area. Methods One hundred and sixty villagers from Yachi Twon using non-improved cooking stoves were selected as the non-intervened group in Bijie City, Guizhou Province where coal-burning fluorosis was prevailing; 153 villagers as the intervented group were chosen from Changchun Twon, where cooking stoves were improved; 151 villagers were served as the control group from Baiyunshan Twon, Changshun County without endemic fluorosis. The activity of GSTs was tested by colorimetric analysis with spectrophotometer. The genotype of the GSTP1 gene Ile105Val polymorphism, presenting as either homozygous wild-type (AA), or heterozygous mutation type (AG), or homozygous mutation type (GG), was detected through the PCR-RFLP procedure. Results The activity of GSTs in plasma of non-intervened group [(12.44±4.97) kU/L]was significantly lower than that of intervened group (P < 0.05), and that of intervened group[(20.78±6.20)kU/L]was significantly lower than that of control group[(24.30±6.27)kU/L, P< 0.05]. The difference of the enzyme activity of three groups were statistically significant (F = 51.71, P < 0.05), but this enzyme activity did not vary significantly in each sex of each grnup(P > 0.05). Compared intervened group [AA:67.3%(103/153), AG:29.4%(45/153),GG:3.3%(5/153)]and non-intervened group[AA:66.9%(107/160), AG:30%(48/160), GG:3.1%(5/160)]with control group[AA:74.8%(113/151), AG:25.2%(38/151), GG:0 (0/151)], the Ile105Val polymorphism site of GSTP1 gene had significant difference(χ2= 6.04,6.07, both P< 0.05), but not significant between intervened and non-intervened groups(χ2 = 0.02, P>0.05). Conclusions Fluorosis can decrease the activity of GSTs and introduce the GSTP1 gene Ile105Val polymorphism, intervention with the fluorine intake will improve the effect of fluoride on the body.     

氟中毒人群过氧化氢酶和谷胱甘肽S-转移酶的活力

目的：探讨燃煤型氟中毒地区人群过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GSTs）活力与氧化应激的关系。方法：于贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取245名村民作为非干预组,于该市长春镇燃煤型氟中毒改灶村抽取240名村民作为干预组,于非地方性氟中毒长顺县白云山镇抽取247名村民作对照组,利用比色法分别测定CAT、GSTs的活力。结果：非干预组血浆CAT活力和GSTs活力低于干预组和对照组,干预组低于对照组,各组间差异有统计学意义（P〈0.05）;各组间CAT、GSTs活力无性别差异（P〉0.05）。结论：过量氟可能使体内自由基代谢失衡,推测通过人为干预氟的摄入,有助于机体恢复氧化平衡。     

贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

目的：探讨贵州省毕节市燃煤型氟中毒地区人群谷胱甘肽硫转移酶（GSTs）活性及GSTP1基因Ile105Val位点多态性。方法：在贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取160名村民作为非干预组,在毕节市长春镇燃煤型氟中毒改灶村抽取153名村民作为干预组,在非病区长顺县白云山镇抽取151名村民作对照组,用比色法分别测定各组GSTs活力;PCR-限制性片段长度多态性（PCR-RFLR）法检测各组GSTP1基因Ile105Val位点多态性[野生纯合型（AA）、突变杂合型（AG）、突变纯合型（GG）。结果：GSTs活力组间比较差异有统计学习意义（F=51.71,P〈0.05）。其中对照组[（24.30±6.27）kU/L]高于干预组[（20.78±6.20）kU/L]、非干预组[（12.44±4.97）kU/L],组间两两比较差异有统计学意义（P均〈0.05）;同组性别间比较差异无统计学意义（P均〉0.05）。GSTP1基因Ile105Val位点多态性,干预组[AA：67.3%（103/153）,AG：29.4%（45/153）,GG：3.3%（5/153）]、非干预组[AA：66.9%（107/160）,AG：30%（48/160）,GG：3.1%（5/160）]与对照组[AA：74.8%（113/151）,AG：25.2%（38/151）,GG：0（0/151）]比较,差异有统计学意义（χ^2=6.04、6.07,P均〈0.05）;干预组与非干预组比较,差异无统计学意义（χ2=0.02,P〉0.05）。结论：氟中毒能导致机体GSTP1基因Ile105Val基因位点的多态性发生变化,人为干预对氟的摄入可改善氟对机体的影响。     

过氧化氢酶基因-262C/T及-21A/T多态性与燃煤型氟中毒相关性的研究

目的 探讨过氧化氢酶(CAT)基因启动子区-262C/T、-21A/T多态性与燃煤型氟中毒之间的关系.方法 2007年,在贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取150名村民作为非干预组,于该市长春镇燃煤型氟中毒改灶村抽取150名村民作为干预组,于非地方性氟中毒长顺县白云山镇抽取150名村民作对照组,应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)分析方法,分别检测各组CAT-262C/T、-21A/T多态的基因型.结果 非干预组、干预组和对照组CAT-262C/T、CAT-21A/T多态的基因型频率分布符合Hardy-weinberg遗传平衡定律(P＞0.05).在研究人群中检测到CAT-262C/T多态的两种基因型CC和CT,未检测到TT基因型;检测到CAT-21A/T多态的三种基因型AA、AT和TT.对照组、干预组和非干预组CAT基因-262C/T多态位点的基因型(CC、CT)频率分别为89.33%(134/150)、10.67%(16/150)、88.67%(133/150)、11.33%(17/150)、9333%(140/150)、6.67%(10/150);对照组、干预组和非干预组C等位基因频率分别为94.67%(284/300)、94.33%(283/300)、96.67%(290/300);T等位基因频率分别为5.33%(16/300)、5.67%(17/300)、3.33%(10/300).对照组,干预组和非干预组CAT基因-21A/T多态位点基因型(AA、AT、TT)频率分别为48.67%(73/150)、46.00%(69/150)、5.33%(8/150)、52.67%(79/150)、38.00%(57/150)、9.33%(14/150)、51.33%(77/150)、38.00%(57/150)、10.67%(16/150);对照组、干预组和非干预组A等位基因频率分别为71.67%(215/300)、71.67%(215/300)、70.33%(2ll/300);T等位基因频率分别为28.33%(85/300)、28.33%(85/300)、29.67%(89/300).CAT-262C/T和CAT-21A/T基因型频率及等位基因频率在对照组,干预组和非干预组中的分布,组间比较差异均无统计学意义(x2=0.331、0.336,P均＞0.05).结论 CAT基因-262C/T、-21A/T多态性与燃煤型氟中毒无明显的相关性.

Abstract：

Objective To explore the relationship between -262C/T and -21A/T polymorphisms of catalase(CAT) gene and coal-burning borne fluorosis. Methods In 2007, 150 villagers were taken as a nonintervention group in Bijie city from the village of coal-burning borne fluorosis areas with unchanged cooking stoves;150 villagers were taken as the intervention group from the town of Changchun county where cooking stoves changed; 150 villagers were taken as control from non-endemic fluorosis areas in Baiyun town of Changshun county.PCR-restriction fragment length polymorphism were employed to detect genotypes of CAT-262C/T and CAT-21A/T polymorphism of CAT gene. Results The genotypic frequencies of CAT-262C/T and CAT-21A/T in nonintervention group,intervention group and control group were in line with Hardy-Weinberg equilibrium law (P＞ 0.05 ).The genotypes of CC and CT were detected while no TT were detected for CAT-262C/T polymorphism; the genotypes of AA, AT and TT were detected for CAT-21A/T. The genotype frequencies of CAT-262 CC, CT in control group, intervention group and non-intervention group were (89.33%(134/150), 10.67%(16/150); 88.67%(133/150), 11.33% (17/150),93.33% (140/150),6.67% (10/150), respectively. The gene frequency of C in control group, intervention group and non-intervention group were (94.67% (284/300), 94.33% (283/300),96.67%(290/300), respectively. The gene frequency of T in control group, intervention group and non-intervention group were 5.33%(16/300), 5.67%(17/300), 3.33%(10/300), respectively. The genotype frequencies of CAT-21 AA,AT and TT in control group, intervention group and non-intervention group were 48.67%(73/150),46.00%(69/150),5.33%(8/150) ,52.67%(79/150) ,38.00%(57/150) ,9.33% (14/150) ,51.33%(77/150) ,38.00%(57/150), 10.67%(16/150), respectively. The gene frequency of A in control group, intervention group and non-intervention group were 71.67%(215/300),71.67%(215/300),70.33%(211/300), respectively. The gene frequency of T in control group, intervention group and non-intervention group were 28.33% (85/300),28.33% (85/300),29.67% (89/300),respectively. CAT-262C/T and CAT-21A/T genotype and allele frequencies in the control group, the intervention group and non-intervention group showed no significant differences in the distribution(x2= 0.331,0.336, all P ＞0.05 ). Conclusion CAT-262C/T and CAT-21A/T polymorphism is not associated with coal-burning borne fluorosis.     

贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

Objective To investigate plasma glutathione S-transferase(GSTs) activity and GSTP1 gene Ile105Val polymorphism in Bijie City, Guizhou Province, a coal-burning fluorosis endemic area. Methods One hundred and sixty villagers from Yachi Twon using non-improved cooking stoves were selected as the non-intervened group in Bijie City, Guizhou Province where coal-burning fluorosis was prevailing; 153 villagers as the intervented group were chosen from Changchun Twon, where cooking stoves were improved; 151 villagers were served as the control group from Baiyunshan Twon, Changshun County without endemic fluorosis. The activity of GSTs was tested by colorimetric analysis with spectrophotometer. The genotype of the GSTP1 gene Ile105Val polymorphism, presenting as either homozygous wild-type (AA), or heterozygous mutation type (AG), or homozygous mutation type (GG), was detected through the PCR-RFLP procedure. Results The activity of GSTs in plasma of non-intervened group [(12.44±4.97) kU/L]was significantly lower than that of intervened group (P < 0.05), and that of intervened group[(20.78±6.20)kU/L]was significantly lower than that of control group[(24.30±6.27)kU/L, P< 0.05]. The difference of the enzyme activity of three groups were statistically significant (F = 51.71, P < 0.05), but this enzyme activity did not vary significantly in each sex of each grnup(P > 0.05). Compared intervened group [AA:67.3%(103/153), AG:29.4%(45/153),GG:3.3%(5/153)]and non-intervened group[AA:66.9%(107/160), AG:30%(48/160), GG:3.1%(5/160)]with control group[AA:74.8%(113/151), AG:25.2%(38/151), GG:0 (0/151)], the Ile105Val polymorphism site of GSTP1 gene had significant difference(χ2= 6.04,6.07, both P< 0.05), but not significant between intervened and non-intervened groups(χ2 = 0.02, P>0.05). Conclusions Fluorosis can decrease the activity of GSTs and introduce the GSTP1 gene Ile105Val polymorphism, intervention with the fluorine intake will improve the effect of fluoride on the body.     

毕节市14例氟乙酰胺急性中毒的调查报告

毕节市水箐镇、田坝镇先后发生14人多次氟乙酰胺急性中毒,其中死亡7人。临档症状典型,实验室检查符合。     

综合治理后燃煤污染型地方性氟中毒病区人群血脂水平改变

目的 检查经综合治理后的燃煤污染型地方性氟中毒(地氟病)病区人群血脂改变(包括总胆固醇、甘油三酯和高密度脂蛋白),寻找经综合防治措施后病区人群病情好转的健康判别指标.方法 2011年,在贵州省毕节市选择燃煤污染型地氟病病区人群,按综合治理时间长短和病情轻重分为4个组,轻病区+治理短组(98例)、轻病区+治理长组(70例)、重病区+治理短组(61例)、重病区+治理长组(164例);在贵阳市选择非地氟病病区人群(125例)作为对照组.两组人群空腹采血,采用酶联免疫吸附试验(ELISA)法测定血清中总胆固醇、甘油三酯和高密度脂蛋白含量.结果 轻病区+治理短组、轻病区+治理长组、重病区+治理短组、重病区+治理长组人群血清总胆固醇水平[(5.75±3.16)、(5.56±2.86)、(7.25±3.84)、(6.51±3.48)mmolL]、甘油三酯水平[(256±1.45)、(2.98±1.50)、(3.20±1.65)、(2.94±1.49)mmol/L]均高于对照组[(3.97±1.80)、(1.25±0.97) mmol/L,P均＜0.05],而高密度脂蛋白水平[(0.72±0.40)、(0.96±0.60)、(0.46±0.27)、(0.81±0.43)mmol/L]明显低于对照组[(1.15±0.67) mmol/,P均＜0.05];在综合治理时间较长的地氟病轻、重病区人群,仅高密度脂蛋白水平较综合治理时间较短的氟中毒轻、重病区人群明显改善(P均＜ 0.05).结论 通过综合治理,地氟病病区人群血清中高密度脂蛋白的水平明显增高,其机制可能与综合治理后减弱氟对心血管的损伤有关,但对血清总胆固醇和甘油三酯水平无明显影响,人体血清中高密度脂蛋白水平可考虑为评估综合治理后病区人群血脂水平改善的指标.     

燃煤型氟中毒地区人群血浆中髓过氧化物酶和谷胱甘肽硫转移酶活力的变化

目的：探讨燃煤型氟中毒地区人群血浆中髓过氧化物酶（MPO）、谷胱甘肽硫转移酶（GSTs）活力与氧化应激的关系。方法：2007年6月至8月在于贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取245名村民作为非干预组,于该市长春镇燃煤型氟中毒改灶村抽取240名村民作为干预组,     

燃煤型氟中毒地区人群血浆中髓过氧化物酶和谷胱甘肽硫转移酶活力的变化

目的探讨燃煤型氟中毒地区人群血浆中髓过氧化物酶(MPO)、谷胱甘肽硫转移酶(GSTs)活力与氧化应激的关系。方法2007年6月至8月在于贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取245名村民作为非干预组,于该市长春镇燃煤型氟中毒改灶村抽取240名村民作为干预组,于非地方性氟中毒病区的长顺县白云山镇抽取247名村民作对照组,利用比色法分别测定血浆中髓过氧化物酶、谷胱甘肽硫转移酶活力。结果非干预组血浆MPO活力>干预组>对照组,各组间差异有统计学意义(P<0.05或P<0.01);非干预组血浆GSTs活力<干预组<对照组,各组间差异有统计学意义(P<0.05或P<0.01)。各组间MPO、GSTs活力无性别差异(P>0.05)。结论过量氟可能使体内自由基代谢失衡,推测通过人为干预氟的摄入,有助于机体恢复氧化平衡。     

贵州省地方性氟中毒地区人群GSTP1基因Ile105Val位点多态性分析

目的 探讨贵州省毕节市燃煤型氟中毒地区人群谷胱甘肽硫转移酶(GSTs)活性及GSTP1基因Ile105Val位点多态性.方法 在贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取160名村民作为非干预组,在毕节市长春镇燃煤型氟中毒改灶村抽取153名村民作为干预组,在非病区长顺县白云山镇抽取151名村民作对照组,用比色法分别测定各组GSTs活力;PCR-限制性片段长度多态性(PCR-RFLR)法检测各组GSTP1基因Ile105Val位点多态性[野生纯合型(AA)、突变杂合型(AG)、突变纯合型(GG)].结果 GSTs活力组间比较差异有统计学习意义(F=51.71,P<0.05).其中对照组[(24.30±6.27)kU/L]高于干预组[(20.78±6.20)kU/L]、非干预组[(12.44±4.97)kU/L],组间两两比较差异有统计学意义(P均<0.05);同组性别间比较差异无统计学意义(P均>0.05).GSTP1基因Ile105Val位点多态性,干预组[AA:67.3%(103/153),AG:29.4%(45/153),GG:3.3%(5/153)]、非干预组[AA:66.9%(107/160),AG:30%(48/160),GG:3.1%(5/160)]与对照组[AA:74.8%(113/151),AG:25.2%(38/151),GG:0(0/151)]比较,差异有统计学意义(χ2=6.04、6.07,P均<0.05);干预组与非干预组比较,差异无统计学意义(χ2=0.02,P>0.05).结论 氟中毒能导致机体GSTP1基因lle105Val基因位点的多态性发生变化,人为干预对氟的摄入可改善氟对机体的影响.