脑疾病患者脑脊液血浆渗透压、pH值及电解质含量的研究

目的 :探讨脑疾病患者脑脊液 ( CSF)渗透压、血浆渗透压、p H值及电解质的变化。方法 :对脑疾病患者 CSF渗透压、血浆渗透压、p H值及电解质进行测定。结果 :脑出血急性期和细菌性脑膜炎患者的 CSF及血浆渗透压、CSF中 K+明显高于正常组 ( P <0 .0 1) ,p H显著低于正常组( P <0 .0 1)。脑梗死患者的 CSF及血浆 Ca2 +明显降低 ( P <0 .0 1)。结论 :监测 CSF、血浆渗透压、p H值及电解质对判断脑疾病患者的病情预后有一定价值。     

重组Persephin腺病毒对6-OHDA损伤神经干细胞的保护作用

[目的]探讨Persephin对6-羟基多巴(6-OHDA)损伤神经干细胞的保护作用.[方法]用RT-PCR法从人胚胎脑获得Persephin cDNA,构建重组Persephin腺病毒.用Western blot方法分析Persephin基因的表达,Hoechst染色和流式细胞术测定6-羟基多巴对神经干细胞凋亡的诱导作用.[结果]从人胎脑成功克隆了人Persephin cDNA,构建了其腺病毒载体.Western blot证实重组Persephin腺病毒在神经干细胞中的表达,表达的Persephin可抑制6-羟基多巴诱导的神经干细胞凋亡.[结论]Persephin对6-羟基多巴损伤神经干细胞具有保护作用.     

重组Persephin腺病毒对6-OHDA损伤神经干细胞的保护作用

【目的】探讨Persephin对6-羟基多巴(6-OHDA)损伤神经干细胞的保护作用。【方法】用RT-PCR法从人胚胎脑获得Persephin cDNA，构建重组Persephin腺病毒。用Western blot方法分析Persephin基因的表达，Hoeehst染色和流式细胞术测定6-羟基多巴对神经干细胞凋亡的诱导作用。【结果】从人胎脑成功克隆了人Persephin cDNA，构建了其腺病毒载体。Western blot证实重组Persephin腺病毒在神经干细胞中的表达，表达的Persephin可抑制6-羟基多巴诱导的神经干细胞凋亡。【结论】Persephin对6-羟基多巴损伤神经干细胞具有保护作用。     

疏风散热胶囊的TLC鉴别

银翘散是常用中药方剂,疏风散热胶囊是在银翘散（贵州一方）［1］的基础止经加工制成的中药胶囊剂,现已收入《卫生部药品标准》中药成方制剂第十二册[2]。本品由金银花、忍冬藤、薄荷、荆芥、地黄、连翘、桔梗、牛辈子、振子、甘草、淡豆鼓、淡竹叶共十二味中药组成,具有清热解毒、疏风散热的功效,用于风热感冒、发热头痛,咳嗽日子、咽喉疼痛的治疗。现有质量标准中鉴别项只有粉末显微及挥发油显色反应。为了进一步控制产品质量,提高质量标准,保证药品疗效,采用TLC（薄层色谱法）鉴别对该品种进行质量控制,为完善质量标准提供依…     

强力枇杷露的TLC鉴别

强力机把露为常用中成药,已收入《卫生部药品标准冲药成方制剂第二册。该药由楷杷叶、罂粟壳、百部、白前、桑白皮、桔梗、薄荷脑组成,具有养阴敛肺、镇咳祛痰之功效,用于久咳劳嗽、支气管炎等［1］。为了有效地控制产品质量,保证药品疗效,采用TLC（薄层色谱法）对该药品进行鉴别,方法简单易行,重现性较好,为完善该药品的质量提供了依据。1实验材料百家露（强力机把露）：贵州民族制药厂生产,批号990403、990301、990802强力机把露罂粟壳阴性对照溶液：按照强力机把露处方和制备工艺,除去罂粟壳,实验室自制。强力批把露薄荷脑…     

高血压及局部脑缺血后AⅡ,ANF在下丘脑和血浆中的含量变化

本实验动态观察了肾血管性高血压鼠不同时期下丘脑和血浆中ＡⅡ和ＡＮＦ的含量变化，并测定了局部脑缺血后高血压鼠和正常鼠梗塞灶的大小以及对下丘脑和血浆中ＡⅡ和ＡＮＦ的影响。结果发现：肾血一高血压形成后，下丘脑和血浆中的ＡＮＦ均明显增加，以高血压早期增加幅度最高。而ＡⅡ在血浆中明显增加，在下丘脑却明显下降（Ｐ＜０．０１）。局部脑缺血后，在高血压鼠形成的梗塞灶（１７６．５９±２３．０３ｍｍ＾３）比对照鼠的（     

不同鼠龄大鼠颈总动脉内膜弹力纤维退行性变性的实验研究

Objective Studying the difference of the uhrastructures of the internal elastic laminae (IEL)of the eommonal carotid artery among young,adult and aged rats in order to set up a efficient preventional and therapeutical strategies to the cerebrovascular disease(common diseases of the cerebral vessels)among aged people.Methods Twenty-four male Wistar rats,aged from 3 months to 3 yeam were used for this study.The IEL in the bifurcation of the right common caotide artery was isolated and digested with hot alkali.The luminal surface of each IEL was scanned by the scanning electric microscope.The number and the area of each fenestration in the IEL were measured and compared among three different age groups.Results The number of the fenestrations was the most in the adult(964.00±4.13),which was significantly more than that in the young(710.00±1.22)or in the aged group(624.00±2.54,P<0.01).The difference of the number of the fenestrations was not significant between aged and young rats(P>0.05).The number of the large fenestrations was remarkable increase with age increasing and the difference between any two groups was all significant[adult(52.40±2.79),young(13.00±1.10),aged(105.20±8.78),P<0.01].The mean percantage area of the fenestrations was also remarkably increasing with age[adult(23.58±2.12),young(15.52±1.70),aged(39.86±3.51),P<0.01].This value was the highest in aged rats which were significantly more than that in the aduh or in the young rats and the difference between adult and young groups was not significant.Conclusions The age-related decrease in elasticity and in the ablitity of preventing penetration of the macromolecules in the IEL maybe a crucial reason for higher incidents of atheroscelerosis and aneunynoma in the bifurcations in the common carotid artery in aged people.     

Orem自理理论在格林巴利综合征患儿中的应用体会

目的总结Orem自理理论在30例格林巴利综合征患儿护理中的应用体会.方法将Orem自理学说中辅助教育,部分补偿性护理、全补偿性护理用于格林巴利综合征患儿的治疗过程中,给患儿家属提供情感、信息支持.结果患儿日常生活能力、心理及社会功能明显改善,生活质量提高.结论Orem自理理论应用于格林巴利综合征患儿可增进患儿自理能力,帮助其主动参与护理,并为他们回归家庭、社会,提高生活质量打下良好基础.     

介绍一种新型卡式输液架

临床现用的立式输液架,不仅体积大、笨重,而且固定把手常因生锈失灵,不能调节高度。为此,我们结合临床实际自行设计出多功能的新型卡式输液架,并获得国家专利,介绍如下: 1 结构选用坚固、耐用的金属材料,由挂钩、支杆、卡环、底座及固定螺杆组成。挂钩可调节长度,支杆其上部为可调节高度的伸缩内杆,(详见附图)。