ZX-5及其光学异构体对兔眼血流和大鼠视网膜功能恢复的影响

目的:研究ZX-5对缺血后视网膜功能的恢复作用,并比较其光学异构体(R,R)-ZX-5和(S,S)-ZX-5对脉络膜血流及缺血后视网膜功能恢复的影响。方法:用彩色微球技术研究兔高眼压下(40mmHg)脉络膜血流的变化。用视网膜电生理仪测量b波,评价大鼠缺血后视网膜功能的恢复情况。结果:10g/L(R,R)-ZX-5滴眼液50μL能在不同时间点提高脉络膜血流(P<0.05),而(S,S)-ZX-5在相同条件下对提高脉络膜血流没有影响。ZX-5和(R,R)-ZX-5在不同时间点对视网膜缺血后功能恢复作用明显(P<0.05),(R,R)-ZX-5的作用优于ZX-5;而(S,S)-ZX-5对缺血后视网膜功能的恢复作用不明显。结论:ZX-5和(R,R)-ZX-5对增加脉络膜血流量和促进视网膜功能的恢复有显著功效,(R,R)-ZX-5恢复视网膜功能的作用更强,有可能进一步开发成有效防治眼血流障碍相关性眼病的药物。     

中医药辨治椎-基底动脉供血不足性眩晕

眩晕症状经常发生的患者中,60%是由于椎-基底动脉供血不足引起.笔者采用天麻钩藤饮合泽泻汤化裁辨治此病52例,取得了满意疗效.现报道如下.     

丙酮酸乙酯注射液的大鼠长期毒性试验

目的： 观察连续肌肉注射丙酮酸乙酯 (ethyl pyruvate,EP)注射液后大鼠产生的长期毒性反应。方法：按年龄、体质量、性别均衡的原则将60只SD大鼠随机分成EP注射液高 (2 400 mg/kg)、中 (1 600 mg/kg)、低 (50 mg/kg)剂量组和溶剂对照组,每组15只,3个给药组每天进行双后肢 (或臀部)肌肉注射给药,连续14 d,溶剂对照组则给予等容量大豆油,观测大鼠一般情况、血常规、血液生物化学等相关毒性反应指标的变化。结果：大鼠每次注射EP药物后5 min内高剂量组产生明显抽搐、俯卧、后肢僵直、呼吸急促等毒性反应但逐渐缓解,注射后30 min内即恢复正常,未见动物死亡;中剂量组上述毒性反应较轻;低剂量组与溶剂对照组无明显差别。血常规和血液生物化学相关指标除在第1次给药14 d后高剂量组的肺和脾系数,中剂量组脾系数明显增加外 (P<0.05),其余各剂量组与溶剂对照组比较差异均无统计学意义 (P>0.05),停药后各剂量组均未见迟缓性毒性发生。结论：高剂量 (2 400 mg/kg) EP注射液对大鼠中枢神经系统会产生较明显毒性作用,但为可逆性的。EP 注射液作为单次用药对大鼠无长期毒性作用。     

收敛法治疗慢性支气管炎50例

<正>广西玉林市人民医院中医科(537000)周丽报道:本组50例,其中男30例,女20例;年龄最大79岁,最小14岁,平均41岁;病程最长30年,最短1年,平均7年。合并不同程度的肺气肿11例,合并肺心病5例。治疗以收敛止咳平喘,兼及益气为法。药用:五味子10～12g,诃子5～10g,罂粟壳10～12g,太子参15～18g,茯苓12～15g,仙鹤草20～30g,菟丝子10～15g。加减:寒痰阻肺加炙麻黄、干姜、半夏;痰热阻肺加桑白皮、黄芩、鱼腥草;肺阴不足加生地黄、沙参、麦冬。每日1剂,水煎分2次服。结果:显效11例,有效34例,无效5例,总有效率为90％。     

优化专家审稿人队伍势在必行

学术质量是学术期刊的核心和生命,也是学术期刊赖以生存与发展的前提条件。要保证学术期刊的学术质量,除了要有高水平的作者队伍、优秀的编辑队伍外,一支高素质、结构优化的专家审稿人队伍也是至关重要的。因此笔者认为有必要从学术类科技期刊的专家审稿人队伍建设入手,探讨提高学术期刊质量的问题。     

Effect of Dexamethasone and Aquaporin-1 Antisense Oligonucleotides on the Aquaporin-1 Expression in Cultured Human Trabecular Meshwork Cells

The changes in the expression of aquaporin-1 （AQP1） mRNA and protein in cultured human trabecular meshwork （HTM） cells treated with dexamethasone and transfected with antisense oligonucleotides （AS-ODN） were studied, and the implication of AQP1 regulation in corticosteroid-glaucoma and the possibility of AS-ODN inhibiting the AQP1 expression were evaluated. The cultured HTM cells in vitro were treated with different concentrations of dexamethasone and transfected with oligonucleotides for 5 days respectively. Then, total RNA and protein of HTM cells were extracted. The changes of AQP1 mRNA and protein were demonstrated qualitatively and quantitatively by RT-PCR and Western blot. Band intensities were detected by imaging analysis. There was a parallel relationship between the results of RT-PCR and those of Western blot. The expression levels of AQP1 mRNA and protein in dexamethasone-treated groups were increased initially and decreased later as dexamethasone concentration was stepped up. In the 0.04 μg/mL and 0.4 μg/mL groups, the levels of AQP1 were higher than in control group （0 μg/mL）. In the 4 μg/ mL and 40 μg/mL groups, the AQP1 expression levels were lower than in control group. AS-ODN could down-regulate the expression of AQP1 mRNA and protein in a dose-dependent manner. At 5 μg/mL, down-regulation efficiency reached the maximum. There was no statistically significant difference in the expression of AQP1 mRNA and protein between all sense oligonucleotides groups and control group. It was suggested that dexamethasone may induce the changes of the AQP1 expression in HTM cells to be involved in the occurrence of corticosteroid-glaucoma. AS-ODN can down-regulate the AQP1 expression in HTM cells to some extent.     

谈中医基础理论实验教学中的心理学问题

近年来,全国不少中医院校的中医基础理论学科相继开设了实验课,这门新的课程不同于我们已经驾轻就熟的课堂教学,因此摸索这门课程的规律,总结出科学的教学方法,已成当务之急。从实验课的特点看,它要求学生掌握一定的实验技能并进行一种新     

温贴灵的稳定性考察

温贴灵系由冰片、肉桂等四味中药精制而成的透皮剂。经临床验证 ,该药外敷神厥穴 ,治疗风寒型及脾虚型腹泻、虚寒腹痛 ,效果明显。冰片芳香走窜、通利诸窍 ,为方中主药。本实验以冰片有效成分变化作为指标 ,用高温加速实验 (初均速法 )和留样观察实验全面考察该药的稳定性。1　仪器与试剂实验仪器　日本岛津ＧＣ——— 8Ａ气相色谱仪 ,美国ＳＰ42 90色谱数据处理机。药物及对照品　冰片标准品 (中国药品生物制品检定所 ) ;化学试剂为分析纯 ;温贴灵 (荆州市中医院制剂室 )。2　方法和结果2 1　冰片的含量测定色谱柱为 10 %ＰＥＧ——— 2 0…