CT对腹膜后肿瘤的诊断体会：附13例分析

ＣＴ检查可以较清楚了解腹膜后肿瘤的大小、部位、范围，及与周围组织器官的关系，有时可提供定性依据。本文分析经手术病理证实的１３例腹膜后肿瘤ＣＴ表现，结合文献复习，以提高对腹膜后肿瘤诊断的准确性，为治疗提供更多的依据。一、资料和方法本组１３例中，男８例，...     

艾滋病毒受抗体包围仍有感染力

艾滋病毒受抗体包围仍有感染力科学家最近发现艾滋病病毒在能抑制其活力的抗体包围下仍具有感染能力，这一发现有助于解释为什么艾滋病病毒难以根除。美国弗吉尼亚英联邦大学的一个科研小组对人体淋巴结内一种称为ＦＤＣ的细胞进行了研究。这种细胞中含有大量艾滋病病毒，...     

现代传媒应慎为酒唱赞歌

酒,源起何时,出自哪个国家,目前尚无确实考证。随着时间的推移,目前,酒的品种却是日新月异的多了起来,饮酒的人更是遍布大地各个角落,或老人,或青年,或少年,或男人,或女人,其人数之多可能超过吸烟的人数。 酒对人体健康的益害如何,世人众说纷纭。 历代文人墨客极度渲染酒的兴     

受微波高频辐射人员外周血5—羟色胺含量的变化

The relationship between 5-HT contents in peripheral blood and power density among workers exposed to microwave or high frequency radiation in the workshops were studied. At the same time, systems and disorder of central nervous system and cardiovascular system were examined. The results of study showed that the average 5-HT content in whole blood for high frequency radiation exposure group was lower than that of the control, but without statistical significance. Value for the microwave exposure group was significantly lower than that of the control (P less than 0.01). The 5-HT content of mechanical maintainers exposed to microwave of high frequency radiation were all lower than the contents of their corresponding control groups (P less than 0.01). But for the mechanic operators as compared with their corresponding control, no significant difference was found (P greater than 0.05). The power density exposed by the maintainers of both tested groups were all higher than that of the operators. The 5-HT contents in the whole blood were inversely proportional to the power density of microwave and high frequency groups. The incidence of neurasthenic syndrome, unsymmetric skin temperature in both limbs and hypotension was higher in the microwave and high frequency radiation groups than that in the control group. The incidence of bradycardia and some abnormal items of electrocardiograph in the microwave group was obviously greater than that in high frequency group, which may be related to different frequency exposed for these two exposure groups. Whether it is related to the contents of 5-HT needs further investigation.     

PDCA循环法在提高肾脏细针穿刺活检组织制片质量中的应用

<正>PDCA循环法是质量管理的一个通用工具,主要用于企业产品质量的改进,近年来逐渐被应用到医院管理领域并取得了实效[1]。在临床病理工作中,对肾脏细针穿刺活检的病理制片质量与诊断准确率的要求较高。为提高肾脏细针穿刺活检组织制片质量的优良率,保证报告的准确性和及时性,我科自2016年8月开始将PDCA循环法应用到肾脏细针穿刺活检组织制片中,针对存在的问题,制定适合本病理科的规范化肾脏细针穿刺活检组织制片流程,对制片质量进行持续改进,经过1年的实践操作,取得了明显成效,现报道     

miR-409-3p靶向癌基因ELF2抑制非小细胞肺癌细胞系A549增殖

目的观察miR-409-3p对非小细胞肺癌细胞系A549增殖的影响及其机制。方法对照组为常规条件下培养的转染阳性对照核苷酸的A549细胞寡核苷酸组。实验组在对照组的基础上采用化学合成miR-409-3p模拟物,脂质体转染构建miR-409-3p高表达A549细胞;经q PCR检测,观察2组48 h后miR-409-3p的表达; MTT法检测细胞增殖;流式细胞计量术检测细胞凋亡和周期的差异。结果 miR-409-3p模拟物转染后,A549细胞中miR-409-3p表达水平显著升高(P0. 05);细胞总凋亡比例为22. 66%±4. 61%,显著高于对照组的7. 78%±1. 95%(P0. 05); G0/G1期为40. 21%±5. 37%,显著低于对照组细胞56. 09%±5. 22%(P0. 05);双荧光报告系统表明癌基因ELF2是miR-409-3p的靶基因,过表达miR-409-3p模拟物后,内源性ELF2蛋白表达水平显著下降(P0. 05)。结论 miR-409-3p可能通过调控ELF2的转录水平抑制非小细胞肺癌细胞A549增殖并促进其凋亡。     

褐藻糖胶对人乳腺癌MCF-7细胞的增殖抑制和凋亡诱导作用

目的：研究褐藻糖胶对体外培养的人乳腺癌MCF-7细胞增殖及凋亡的影响，并探讨其凋亡机制。方法:不同浓度(100 mg/L、300 mg/L、500 mg/L、1 000 mg/L)褐藻糖胶处理MCF-7细胞48h后，应用四甲基偶氮唑蓝（MTT）法检测细胞的增殖；Hoechst 33258染色、琼脂糖凝胶电泳法观察细胞的凋亡的形态学及生化改变；逆转录聚合酶链反应（RT-PCR）和蛋白印迹分别测定细胞〖STBX〗bcl-2〖STBZ〗和bax mRNA及其蛋白的表达。结果:不同实验浓度(100 mg/L、300 mg/L、500 mg/L、1 000 mg/L)的褐藻糖胶均能抑制MCF-7细胞的增殖（P<0.01），抑制率呈浓度依赖性增大；褐藻糖胶诱导MCF-7细胞凋亡数增加，且可见明显的、凋亡特有的DNA梯形条带；在褐藻糖胶存在下，〖STBX〗 bcl-2〖STBZ〗 mRNA和蛋白表达减少，而bax mRNA和蛋白表达增加，Bcl-2/Bax比值下降（P<0.05）。结论:褐藻糖胶可抑制MCF-7细胞增殖，且诱导其凋亡，其凋亡机制是下调Bcl-2和上调Bax蛋白的表达。