实验性视网膜光化学损伤中的蛋白激酶C

目的：研究实验性视网膜光化学损伤中蛋白激酶C(protein kinase C，PKC)活性的改变；探索地塞米松(dexamethasone，DXM)对视网膜中PKC活性的影响。 方法；48只SD大鼠分为对照组和DXM组，后者于光照前3天开始，连续5天腹膜腔注射DXM 1mg/(kg·d)。经(1 900±106.9)Ix的绿色荧光灯(λ＝510nm～560nm)连续照射24小时后的6小时和1、3、7、14天，进行视网膜PKC活性检测。 结果：光化学损伤后视网膜中的PKC活性在短暂的上升后即出现持续性的下降；DXM对PKC的活性改变无明显作用。 结论：光化学损伤中的视网膜功能障碍可能与PKC活性的持续降低有关。 （中华眼底病杂志，1997，13：78-80）     

玉屏风散联合阿奇霉素治疗小儿反复急性上呼吸道感染

急性上呼吸道感染(acute upper Respiratoinginfection)为小儿时期常见病、多发病,一年四季均可发病,每人每年可发病数次。急性上呼吸道感染病原体主要侵犯鼻、咽、扁桃体及喉部而引起炎症。我院于2004年7月~2006年5月,应用玉屏风散联合阿奇霉素分散片治疗44例反复上呼吸道感染患儿,并观察其治疗效果,现报告如下。1资料与方法1.1一般资料根据1987年全国小儿呼吸道疾病会议制定的诊断标准,确诊为上呼吸道感染患儿共44例,男24例,女20例。年龄4~11岁,平均年龄9.5岁,在此次患病前3个月中有≥2次的急性上呼吸道感染发生,每次发生的病程≥2d,本次…     

综合针刺法在胸外科手术后镇痛效果观察

本组30例胸外科术后采用综合针刺珐镇痛、先后应用温针、腕针、耳针、水针。以杜冷丁25毫克肌注作自身对照。用钾离子测痛和血液循环功能监护仪来考核镇痛效果和调整功能。实验结果：1、痛阈提高以腕针最为明显，耐痛阈提高出现递进、叠加镇痛效果。2．各种针刺法均比杜冷丁镇痛效果显，P<0．01。3．针后脉率减慢，心肌收缩力加强、搏出量增加。     

辛伐他汀对CD40L介导的ECV-304细胞E选择素表达及粘附功能的影响

目的研究在人脐静脉内皮细胞中3-羟-3-甲基戊二酰辅酶A(HMG-CoA)还原酶对CD40/CD40L系统介导的粘附分子表达的影响,及对淋巴细胞与内皮细胞的粘附功能的影响。方法采用人脐静脉内皮细胞株ECV-304,CD40L干预,使之与其表面的CD40作用,用不同浓度辛伐他汀或辛伐他汀(5μmol.L-1)+甲羟戊酸干预,流式细胞术和RT-PCR检测干预前后E选择素(E-Selectin)的表达,流式细胞术检测CD40L介导的淋巴细胞与内皮细胞的粘附功能。结果不同浓度辛伐他汀可下调CD40L介导的E-Selectin的表达,并呈一定的剂量依赖性。甲羟戊酸(400μmol.L-1)抑制了辛伐他汀(5μmol.L-1)对E-Selectin表达的下调作用。辛伐他汀可降低CD40L介导的淋巴细胞与内皮细胞的粘附功能。结论他汀类药物的抗炎作用与抑制CD40-CD40L系统有关,其机制是通过抑制HMG-CoA还原酶而发挥作用的。     

医院感染漏报因素分析

为了提高医院感染发病率监测的准确性,笔者在近二年的工作中对医院感染漏报现象进行了调查,并了解其相关因素,现报告如下。     

放射线联合p53基因及内皮抑素治疗小鼠前列腺癌皮下移植瘤

Objective To test the hypothesis that p53 gene therapy combined with endostatin can enhance tumor response to radiation therapy of RM-1 mouse xenograft prostate cancer and to investigate its mechanism. Methods A mouse prostate cancer model was established. Then mice with xenograft tumor were randomly divided into group A (control), B (radiation), C (radiation and rAdp53), D (radiation and rh-endostatin) and E (radiation and rAdp53 and rh-endostatin). On day 1, rAdp53 was injected intra-tumorously with 1 × 1010 vp per animal to group C and E. From day 1 to 14, rh-endostatin was given 15 mg/kg intraperitoneally daily to group D and E. On day 4 single fraction of 15 Gy was given to tumors in groups B, C, D and E. Normal saline was injected intra-tumorously or intraperitoneaUy accordingly as control. No treatment was done to group A. Tumor volume was measured daily. Samples were collected on Days 5, 10 and 15. Ki67, CD31, p53 and VEGF were detected by means of immunohistochemistry. Results (1) Radiation alone, radiation combined with intra-tumorous injection of Adp53 and/or intraperitoneal injection of rh-endostatin resulted in tumor growth arrest of RM-1 cells in vivo (P = 0.000). Radiation combined with both rAdp53 and rh-endostatin was the most effective treatment (P < 0.05). (2) All the four treatment groups had a decreased expression of mutant type P53 (P = 0.000). The expression of Ki67 in groups B and C were equal (P 0.05) and increasing (P = 0.000), respectively. Group D had a up-down-up curve (P < 0.05), but group E had a up-down one. On day 5 the expresion of VEGF in group E was the lowest (P < 0.05). An increased expression of MVD compared with the control was shown, and MVD in groups C, D and E were always higher than that in the control (P < 0.05). Conclusions The limitation of radiotherapy could be overcome by combination with beth p53 gene therapy and endostatin on the growth of mouse prostate cancer cell. Radiation, rAdp53 and endostatin have their own role but they can be interacted with each other.     

自理理论在护理产前子痫患者中的应用

在产前子痫患者护理中,我们应用了自理理论并取得了良好的效果,现将我科对2000年2月～2002年8月收治的36例产前子痫患者的护理,介绍如下。 1 入院时护理 产前子痫患者大部分昏迷、自理能力丧失,应用全补偿系统来满足病人自理需要。全补偿系统是指个体活动受限,全部需求由护士予以满足。首先     

冷冻、微波及微波联合干扰素治疗尖锐湿疣疗效观察

目的：观察冷冻、微波及微波联合干扰素治疗尖锐湿疣的临床效果。方法：将204例尖锐湿疣患者随机分为三组，分别给予冷冻、微波及微波联合干扰素治疗，观察治疗后临床疗效及其复发串。结果：微波联合干扰家组的复发率为10．0％，明显低于冷冻组33．3％和微波组20．6％(P＜0．05)。结论：微波联合干扰素治疗尖锐湿疣能提高治愈率，减少复发率。     

外伤性白内障术后二期人工晶状体植入术

目的 探讨外伤性白内障术后二期植入后房型人工晶状体的时机和手术方式。方法 对34例(34只眼)外伤性白内障术后无晶状体眼根据不同情况采用直接睫状沟植入法或缝线固定法进行二期植入后房型人工晶状体，观察两组患者的术后反应和治疗效果。结果 直接睫状沟植入法14只眼(41．2％)，缝线固定法20只眼(58．8％)，31只眼(91．2％)视力达到0．1以上，21只眼(61．76％)视力达0．3以上，28只眼(82．4％)术后矫正视力等于或高于术前最佳矫正视力。结论 选取合适的时机和方式二期植入人工晶状体，有助于减少术后并发症，提高视功能的恢复。