Daidzein affects proliferation and apoptosis in non-small cell lung cancer cells:role of p53 signaling pathway北大核心CSCD

Aim To investigate the effects of daidzein（DD） on the proliferation and apoptosis of non-small cell lung cancer cells,with a focus on the possible role of the p53 signaling pathway in this regard. Methods CCK-8 method and flow cytometry were used to detect the effects of soy isoflavone crude extract and DD on the viability and apoptosis of HELF and H1299 cells. Gene microarray was used to detect the changes in gene expression after treatment of H1299 cells with DD. GSEA and differential analysis were used to screen the major pathways and key genes. RT-qPCR and Western blot were performed to verify the differences in mRNA and protein expression of key genes（p53 and CASP9） in the major pathways. After p53 inhibitor Pifithrin-α inhibited the expression of p53,the effect of DD on p53 mRNA and protein expression levels was examined,and the proliferative effect on H1299 cells was observed. Results Soy isoflavone crude extract and DD promoted proliferation and inhibited apoptosis of normal lung cells and inhibited proliferation and promoted apoptosis of lung cancer cells. p53 signaling pathway was significantly enriched in the DD-treated group（NES=1.78,P=0.000）,and the expressions of p53 and CASP9 genes were found to be significantly up-regulated in the treated group. Compared with the control group,mRNA expression of CASP9 and p53 significantly increased in both HELF and H1299 cells treated with DD（P<0.05）,and p53 protein expression also increased in HELF cells（P<0.05）. After inhibition of p53 expression,DD significantly increased the mRNA expression of p53 in H1299 and HELF cells（P<0.05） and also markedly increased the expression of p53 protein in H1299 cells（P<0.05）,and it was observed that DD inhibited the proliferation of lung cancer cells. Conclusions DD inhibits the proliferation and promotes the apoptosis of lung cancer H1299 cells,and the mechanism mainly involves the p53 signaling pathway. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

某海边防部队官兵健康状况

目的调查某海边防部队官兵的健康状况,为有效地开展医学保健工作提供依据。方法在某沿海边防部队,应用整群抽样方法抽取调查对象共6912人,采用自行设计的健康调查表进行问卷调查与分析。调查表含有与血液系统、消化系统、神经系统疾病相关的20项自觉症状。结果在所调查的自觉症状中,发生率居前5位的症状依次是烦躁(45.0%)、乏力(40.5%)、情绪低落(39.5%)、头晕(37.4%)和失眠34.2%;不同职别、不同军种、不同服役期、不同文化程度、不同地区、不同性别官兵的常见5种症状的发生率明显不同。结论部分官兵的健康状况存在一定问题,应针对职别、军种、服役期、文化程度、地区、性别的特点开展有效的医学保健工作。     

舟山地区部队官兵贫血的流行病学调查

目的了解舟山地区部队官兵贫血的检出情况。方法运用整群抽样方法,采集陆、海军男性官兵的静脉血2mL,检测血红蛋白（Hb）,并开展问卷调查。结果 444名受检官兵的Hb为（144.4±12.1）g/L,Hb〈120g/L的男性共5名,贫血检出率为1.1%,1例为中度贫血。问卷调查表中与血液系统疾病相关的10项自觉症状中,乏力、烦躁最多见;与消化系统疾病相关的10项自觉症状中,食欲不振最多见,且贫血官兵均有不同程度的不良饮食习惯。结论舟山地区部队官兵的贫血检出率较低,但中度贫血被检出;贫血官兵均有不同的不良饮食习惯;乏力、烦躁等自觉症状的发生,有助于贫血的早期诊断与治疗;消化系统自觉症状与贫血的相互关系值得进一步探讨。     

某三级综合医院专病门诊诊疗工作分析

目的了解某三级综合医院专病门诊的诊疗工作现状,分析专病门诊患者需求及管理模式的特点。方法对2008—2011年某三级综合医院专病门诊量进行描述性分析、线性回归分析及相关性分析。结果加强专病门诊管理有利于提高医疗质量和水平,简化门诊流程,实施疾病全程式规范化管理与治疗,提高门诊医疗服务效率。结论近年来专病门诊已逐渐成为门诊工作的增长点,为适应学科发展及患者需求,需不断规范对专病门诊的管理与建设。     

海上医疗救护体系的分析与思考

回顾国内外海上医疗救护体系发展史,介绍了国内海上医疗救护理论体系及现状,分析了组织架构和分级救治体系,指出目前海上医疗救护体系存在的问题,提出了发展建议,有助于促进军队海上医疗救护体系的不断完善.     

重组腺病毒Ad-EGFP-mdr-1转染大鼠骨髓细胞模型的构建

目的 构建转染多药耐药基因-1 （multidrug resistance gene-1,mdr-1）的骨髓细胞.方法 将重组腺病毒Ad-EGFP-mdr-1转染大鼠骨髓细胞.RT-PCR法和Western blot法检测mdr-1 mRNA及蛋白的表达水平 柔红霉素排出试验检测转染mdr-1基因后的骨髓细胞有无功能表达.结果 骨髓细胞病毒转染率为30.14%,存活率为89.25%,转染后骨髓细胞mdr-1的mRNA及蛋白质水平都存在功能性表达,导入的mdr-1表达产物P-gp有药物外排泵功能.结论 转染mdr-1的大鼠骨髓细胞模型的成功构建,为进一步研究肿瘤的多药耐药提供实验基础.     

高血压与膳食和体力活动相关关系分析

[Objective] To understand the status of hypertension among rural adults in Hebei Province and the correlation between diet, physical activity and hypertension, to provide evidence for intervention on hypertension. [Methods] A question-naire investigation on dietary and physical factors of hypertension was conducted among people aged 18 and over in rural area. [Results] 2 407 adults were interviewed and physically measured. The crude prevalence of hypertension was 32.1% and that of men and women respectively was 33.3.0%; 29.5%. The results of multivariate Logistic regression analysis showed that the main risk factors influencing hypertension were excessive intakes of salt and fat, long-term static living. [Conclusions] Rational dietary pattern （low intakes of salt and fat） and physical activity are important measures for prevention on hypertension.     

浅谈中药炮制现状

中药炮制品是我国历代医学家在从事长期的医疗活动中逐步总结发展起来的一项具有传统特色的制药技术．广义的中药炮制是指药材净制、切制或炮炙操作而制成一定规格的饮片,以适应医疗要求及调配、制剂的需要,保证用药安全和有效;而狭义的中药炮制则着重于强调按照中医理论体系和一定的工艺流程使饮片增强疗效、减少毒副作用而满足于临床需求。相反,不恰当不规范的炮制则会达不到预期的临床疗效．现将分述如下。     

中药治疗急性冠脉综合征合并焦虑症58例

2003年1月～2005年8月,笔者在西药治疗急性冠脉综合征(ACS)的基础上,加用中药治疗急性冠脉综合征合并的焦虑症,并与抗焦虑西药阿普唑仑作对照,现报道如下。1临床资料     

CT对早期脂肪肝诊断的意义

脂肪肝是最常见的弥漫性肝病,常缺乏特异的临床表现,其发病机理不明,少数可发展成肝硬化。如早期诊断、早期治疗可防止脂肪肝进一步发展,甚至可使其逆转,通过对35例CT确诊的脂肪肝分析,发现CT因对密度差别敏感性很高,在早期诊断脂肪肝、治疗后随访及鉴别诊断方面均优于B超及实验室检查。