等容血液稀释疗法治疗原发性高血压32例临床分析

我院于1998·l卜1999－09,采用等容血液稀释疗法治疗原发性高血压病32例,并随访6个月,取得较好疗效,现报道如下．l资料与方法1．l临床资料本组32例高血压病中,男24例,女8例,年龄3ot72岁,平均57．6岁;病程5－30年,平均11．5年;高血压病11期25例,ffi期7例．其中合并糖尿病2例,脑梗塞5例,脑动脉硬化症8例,冠心病2例．均按1978年WHO制定的标准并排除继发性高血压．1．2治疗方法①入院确诊后接长期服用降压药的维护量治疗,血液稀释治疗一周,根据血压逐步减量或停用降压药,若舒张压来降至！2．7kPa,降压药维持量不变。等容血…     

蛋氨酸合酶基因A275 6G位点多态性与非综合征型唇腭裂的关联性研究

Objective To study the association of the A2756G polymorphism of the methionine synthase (MS) gene with nonsyndromie cleft lip with or without cleft palate (NSCL/P) in Chinese. Methods Ninety-seven NSCL/P case-parent triads were selected as the case group. One hundred and four healthy subjects and their biological parents were selected as control group. For all subjects the A2756G polymorphism of the MS gene was examined by PCR-RFLP method. Results There was no statistical difference in genotype and allele frequencies for MS A2756G variants among family members between case group and control group. The GG genotype was not detected in the offsprings and mothers. The odds ratio and confidence interval of genotype AG in offspring, father and mother were 1.78(0.74-4.34), 0.80(0. 36-1.79) and 1.26(0. 54-2.93) respectively. The odds ratio and confidence interval of allele G in offspring, father and mother were 1.70(0.78-3.73), 0. 88(0. 49-1. 75) , and 1.23(0.59-2.60) respectively. The G allele did not increase the risk of NSCL/P. Transmission disequilibrium test (TDT) analysis yielded no evidence of linkage disequilibrium (χ2=0.034,P>0. 05). The results of haplotype-based haplotype relative risk (HHRR) analysis (χ2=0.03,P>0.05) and family-based association tests (FBAT) (Z=0. 186, P> 0.05) failed to show association between the MS A2756G variant and the risk of NSCL/P. Conclusion The A2756G polymorphism of the MS gene was not associated with NSCL/P in Chinese in the present study.     

非直属附属医院在医学教育中的作用

非直属附属医院的建立是医学教育的发展的必然,通过对地方优势的综合医院进行系列培训使其成为医学教育的基地,是解决扩招后,医学生大幅增加,生均床位不足的困境的快捷方式,同时该举措也是提升地方医院教学、科研、医疗水平及影响力的有效途径。     

实现应用型高等医学院校实践教学目标的探索

伴随现代医学模式由传统的生物医学模式向生物-心理-社会医学模式的转变、国际医学标准的引入以及高等教育的大众化进程,社会对医疗人才的需求发生了深刻的变化。     

大鼠肠内容物细菌基因组DNA提取方法的比较

目的 比较两种肠内容物前处理和两种提取方法对清洁级SD大鼠肠内容物细菌基因组DNA提取效率.方法 分别选用PBS多次离心漂洗、液氮破细胞两种前处理方法和酚/氯仿抽提、试剂盒过柱法两种提取方法进行组合分析,对4份肠内容物和16份含金黄色葡萄球菌肠内容物进行随机提取.结果 大鼠肠内容物细菌基因组DNA含量和纯度测定结果显示,与PBS反复离心相比,液氮研磨前处理能显著提高大鼠肠内容物基因组DNA.荧光定量PCR表明,液氮研磨前处理较PBS反复离心能更好地收集细菌基因组DNA,其Ct值最低.结论 研究结果表明,采用液氮研磨试剂盒法在大鼠肠内容物DNA提取中是较为优良的方法,该方法为建立实验动物中微生物的定量PCR检测方法打下了基础.     

治疗颈椎病致脊髓前动脉综合征6例临床分析

<正>2000年1月至2010年1月共收治颈椎病致脊髓前动脉综合征患者6例,现总结报告如下。1资料与方法1.1临床表现:本组6例,男性4例,女性2例,年龄41～67岁,平均56.2岁。均具有明确的颈椎病病史14～30年。起病方式:5例在典型的脊髓颈椎病基础上无明显的诱因短期内症状急剧加重,另1例逐渐加重。特征性体征表现为病变平面以下出现"感觉分离"现象,即痛温觉感觉消失或减退,但本体感觉、振动觉及二点分辨觉存在,下肢     

MTHFR基因C677T多态性与非综合征型唇腭裂的相关性研究

目的：探讨MTHFR基因C677T多态性与非综合征型唇腭裂（nonsyndromic cleft lip with or without cleft pa late,NSCL/P）相关性。方法：收集97个核心家庭和104个对照家庭,用聚合酶链式反应-限制性片段长度多态性方法,进行MTHFR基因C677T位点多态性检测;用人群关联研究、传递不平衡检验（transmission-disequlibrium test, TDT）、单倍型相对风险率（haplotype-based haplotype relative risk, HHRR）、家系为基础的关联检验（family-based association test, FBAT）等进行统计分析。结果：人群关联研究分析表明,子代、父亲、母亲的病例组和对照组之间基因型和等位基因的分布差异无统计学意义（P>0.05）;子代、父亲、母亲组,CT基因型携带者相对于CC基因型携带者的OR分别为1.02(95%CI 0.47~2.21)、0.62(95%CI 0.29~1.32)、0.66(95%CI 0.31~1.40);TT基因型携带者相对于CC基因型携带者的OR分别为1.10(95%CI 0.44~2.74)、0.95(95%CI 0.39~2.32)、0.68(95%CI 0.28~1.66);T相对于C基因的OR分别为1.07(95%CI 0.72~1.58)、0.98(95%CI 0.66~1.46)、0.83(95%CI 0.56~1.24)。携带有突变基因T并不能增加患NSCL/P的危险。病例组核心家庭分析表明,TDT检验χ²=1.817,P>0.05;HHRR检验χ²=1.76,P>0.05;FBAT检验Z=1.348,P>0.05。结论：没有发现MTHFR C677T 多态性和NSCLP无关联性,该基因位点的变异可能不会导致中国东北地区人群NSCLP的发生。     

生物净化前后长爪沙鼠微生物和寄生虫携带情况的比较

目的 比较生物净化前后长爪沙鼠微生物携带情况.方法 采集生物净化前后长爪沙鼠的气管、回盲部样本,经血平皿培养后,分离菌落进行纯培养,利用细菌鉴定仪进行菌种鉴定;气管经研磨后,转入选择性培养基鉴别培养;分离血清进行泰泽病原体抗体检测.结果 生物净化后F1代、F2代和F3代长爪沙鼠泰泽病原体血清抗体检测均为阴性,生物净化后的其他微生物和寄生虫普查结果也表明,各项检测中均未发现清洁级啮齿类动物中需排除的病原微生物和寄生虫.结论 剖宫产和代乳技术能有效减少长爪沙鼠携带的微生物和寄生虫.     

蛋氨酸合酶基因A275 6G位点多态性与非综合征型唇腭裂的关联性研究

Objective To study the association of the A2756G polymorphism of the methionine synthase (MS) gene with nonsyndromie cleft lip with or without cleft palate (NSCL/P) in Chinese. Methods Ninety-seven NSCL/P case-parent triads were selected as the case group. One hundred and four healthy subjects and their biological parents were selected as control group. For all subjects the A2756G polymorphism of the MS gene was examined by PCR-RFLP method. Results There was no statistical difference in genotype and allele frequencies for MS A2756G variants among family members between case group and control group. The GG genotype was not detected in the offsprings and mothers. The odds ratio and confidence interval of genotype AG in offspring, father and mother were 1.78(0.74-4.34), 0.80(0. 36-1.79) and 1.26(0. 54-2.93) respectively. The odds ratio and confidence interval of allele G in offspring, father and mother were 1.70(0.78-3.73), 0. 88(0. 49-1. 75) , and 1.23(0.59-2.60) respectively. The G allele did not increase the risk of NSCL/P. Transmission disequilibrium test (TDT) analysis yielded no evidence of linkage disequilibrium (χ2=0.034,P>0. 05). The results of haplotype-based haplotype relative risk (HHRR) analysis (χ2=0.03,P>0.05) and family-based association tests (FBAT) (Z=0. 186, P> 0.05) failed to show association between the MS A2756G variant and the risk of NSCL/P. Conclusion The A2756G polymorphism of the MS gene was not associated with NSCL/P in Chinese in the present study.     

长爪沙鼠的生物净化技术

目的实现长爪沙鼠微生物质量升级和实验动物化目标,需对长爪沙鼠实施生物净化。方法在长爪沙鼠生物净化过程中,采取孕鼠选择和临产期判断、无菌剖宫产、仔鼠代乳以及独立通风笼盒(IVC)饲养管理等措施,比较了ICR小鼠、SD大鼠、长爪沙鼠代乳和饲料中添加营养成分对仔鼠代乳的影响。结果本研究共实施长爪沙鼠无菌剖宫产达85胎次,通过ICR代乳鼠代乳并成功断乳167只。结论本研究顺利实现了长爪沙鼠生物净化,形成的关键技术有望为我国特色实验动物资源的生物净化提供参考。