Observation on Effects of Aconitine via Acupoint Injection in Rabbits

Objective:To investigate the ability of the pericardium meridian(PM) to mitigate or enhance the cardiotoxic effects of aconitine injected at specific acupoint and non-acupoint sites in rabbits.Methods:This study consisted of 3 experiments that were designed to test the effects of injection of 30μg/kg of aconitine at acupoints on the PM(Test 1),at non-acupoint sites on the PM(Test 2),and at acupoints on other meridians and non-meridian sites(Test 3).In Test 1,24 rabbits were randomly assigned to receive injections at Quze (PC3),Tianquan(PC2),or intramuscularly.In Test 2,24 rabbits were randomly assigned to receive injections of aconitine at non-acupointⅠ,non-acupointⅡ,or intramuscularly.In Test 3,48 rabbits were randomly assigned to receive injections at Neiguan(PC6),Sanyinjiao(SP6),Yangjiao(GB35),a non-meridian and non-acupoint site (NMNA),intravenously,and intramuscularly.Electrocardiographs of the rabbits were performed before,during and after injection to determine the incidence of arrhythmia,latency of ventricular rhythm,and recovery rate after aconitine injection.The recovery time index and extent of arrhythmia scores were calculated.Results:In all groups the incidence of arrhythmia was 100%,and the latency of ventricular rhythm was less than 30 min.In Tests 1 and 2,the recovery rates of the Quze and non-acupoint II groups were significantly higher than those of the muscular group(P<0.05).In Test 3,the recovery time index and extent of arrhythmia scores of the Neiguan group were low.There were no significant differences between the other acupoint groups,or the NMNA group, when compared with the group receiving aconitine intramuscularly.Conclusions:Acupoints or non-acupoints along the PM could reduce the severity of the arrhythmia induced by aconitine in healthy rabbits.Meridians play an important role in protecting body functions.     

太子参复方对脾虚自汗大鼠的止汗作用及其机制研究

目的:观察太子参复方对脾虚自汗大鼠的止汗作用并探讨其止汗机制。方法:采用利血平致脾虚大鼠的方法建立自汗模型,采用着色法观察太子参复方对脾虚模型大鼠足趾汗液分泌的影响作用,分别用Western Blot法和RTPCR法检测大鼠脑组织中乙酰胆碱受体(AchR)及其受体m RNA的表达。结果:与模型对照组比较,太子参复方高、中剂量组能减少脾虚模型大鼠的汗点数目(P <0.05);太子参复方高、中剂量组脑组织中AchR蛋白的表达明显下降(P <0.01);各给药组脑组织中AchR mRNA表达显著降低(P <0.01)。结论:太子参复方具有一定的止汗效果,其止汗机制可能是通过抑制脑组织中AchR的蛋白表达和大脑神经递质AChR基因的表达,从而抑制人外泌汗腺腺上皮细胞分泌汗液。     

畲药“十二时辰”水煎剂的急性毒性实验研究

目的:研究畲药"十二时辰"水煎剂对小鼠的急性毒性,以初步评价其安全性。方法:以不同剂量"十二时辰"水煎剂对小鼠灌胃,观察毒性症状,记录死亡情况,并计算最大耐受量(MTD)、最小致死量(MLD)及半数致死量(LD50)的值。结果:小鼠口服"十二时辰"水煎剂的最大耐受量(MTD)为27.44g/kg,最小致死量(MLD)为39.2g/kg,半数致死量(LD_(50))为60.08g/kg。结论:"十二时辰"临床应用时,需严格控制口服剂量。     

松郁安神方对对氯苯丙氨酸致失眠大鼠血浆中前列腺素D2的影响

目的:观察松郁安神方对对氯苯丙氨酸(Para-chlorophenylalanine,PCPA)致失眠模型大鼠血浆中内源性睡眠促进物质前列腺素D2(Prostaglandin D2,PGD2)的影响.方法:采用腹腔注射对氯苯丙氨酸(PCPA)建立失眠大鼠模型,用高、中、低剂量松郁安神方进行治疗,通过酶联免疫吸附法(ELISA)测定大鼠血浆中前列腺素D2(PGD2)的含量.结果:中、高剂量松郁安神方可提高PCPA失眠模型大鼠血浆中PGD2含量(P<0.05或P<0.01).结论:松郁安神方改善睡眠的机制之一可能是通过调节内源性睡眠促进物质PGD2发挥作用.     

蛇葡萄素对人乳腺癌MCF-7细胞增殖与凋亡的影响

目的研究蛇葡萄素对人乳腺癌MCF-7细胞增殖与凋亡的影响。方法用MTT比色法检测蛇葡萄素对MCF-7细胞的增殖抑制作用,绘制量效曲线并计算IC50,流式Annexin V/PI双染法检测细胞凋亡率。结果蛇葡萄素能明显抑制MCF-7细胞增殖,IC50为19.15μg/mL;蛇葡萄素对MCF-7细胞作用6 h后,细胞凋亡率显著增加,且呈量效关系。结论蛇葡萄素能显著抑制MCF-7细胞的增殖,诱导细胞凋亡。     

复方镇痛膏对吗啡依赖大鼠自然戒断征的抑制作用研究

为观察中药复方镇痛膏对吗啡依赖大鼠戒断综合征的疗效,采用剂量递增方式皮下注射吗啡建立吗啡依赖的大鼠模型,观察大鼠每日体重下降百分率和摇头抖体的次数。结果显示复方镇痛膏明显阻止戒断大鼠的体重下降,减少其摇头抖体次数,说明复方镇痛膏能明显抑制吗啡依赖大鼠戒断反应。     

木姜子根多糖对荷瘤小鼠作用的实验研究

从木姜子根中提取多糖进行药理实验，结果表明木姜子根多糖对荷瘤小鼠的肿瘤生长有一定的抑制作用，同时对动物机体的免疫功能有升高趋势，说明木姜子根具有扶正祛邪的作用。     

藤茶总黄酮体外抗肿瘤实验研究

目的观察藤茶总黄酮在体外对肿瘤的影响作用。方法应用四甲基偶氮唑蓝法研究藤茶总黄酮对肿瘤细胞体外增殖的影响。结果藤茶总黄酮对人乳腺癌细胞MCF-7、人前列腺癌细胞PC3和人前列腺癌细胞LNcap的抑制作用都比较强,且剂量依赖性比较明显,随着药物浓度的增大抑制率也随之增大。72 h半数抑制浓度IC50分别为19.15、163.98、27.54μg/mL。结论藤茶总黄酮在体外对人乳腺癌和人前列腺癌有明显的抑制作用。     

雷公藤多苷对肺纤维化大鼠的干预作用

目的 观察雷公藤多苷对肺纤维化大鼠肺形态学、凝血酶原时间、趋化因子及细胞凋亡的影响,探讨其作用机制.方法 大鼠气管内注入博来霉素造模,随机分为模型组、雷公藤多苷高剂量组、低剂量组、泼尼松组、空白组,造模次日起每天给药,28d后处死大鼠,观察大鼠肺形态学变化、血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、血...