煎煮方法和药物配伍对附子毒性的影响

目的:探讨煎煮时间、煎煮溶剂和配伍对附子毒性的影响。方法以心律失常为指标,考察煎煮时间、煎煮溶剂和与甘草、干姜配伍对附子毒性的影响。同时,采用HPLC法测定附子各种不同处理方法中乌头类生物碱的含量。结果煎煮时间延长、醇提和与干姜、甘草配伍均能使附子的毒性降低,附子的毒性与3号色谱峰位置的生物碱呈显著相关性(r=0.91)。结论煎煮时间、煎煮溶剂和配伍对附子毒性均有影响,而3号色谱峰位置的生物碱可能是总乌头类生物碱中毒性较强的物质。     

复方血栓通胶囊对实验性犬缺血心肌的保护作用

目的:复方血栓通胶囊的功效为活血化淤、益气养阴,既往多用于眼科疾患,但临床也有扩大运用于心血管系统疾病的报道。为了证实其药理作用,我们观察了该药对实验性犬缺血心肌的保护作用。方法:采用结扎冠状动脉左前降支的方法复制急性心肌缺血模型。实验过程中取静脉血检测 SOD、MDA、乳酸、乳酸脱氢酶(LDH)等生化指标。结果:血栓通高、低剂量组血浆 SOD 活性均显著高于缺血组(结扎30min 后由缺血组的204.3 U/ml 分别上升至254.1、246.8 U/ml),与对照组和地奥组差异无显著性;其血浆 MDA、乳酸含量及 LDH 活性均显著低于缺血组(结扎30 min 后 MDA 由缺血组的5.84μmol/L 分别下降至3.77、4.00μmol/L;结扎120 min 后乳酸由缺血组的13.58 mol/ml 分别下降至9.44、8.75 mol/ml;结扎30min 后LDH 活性由缺血组的502.1 U/ml 分别下降至221.4、311.0 U/ml),与对照组和地奥组无显著差异。结论:复方血栓通胶囊对实验性犬缺血心肌具有保护作用。     

缺血预处理-后处理对大鼠肠缺血再灌注损伤的影响

Objective To evaluate the effects of ischemic preconditioning-postconditioning on the intestinal ischemia-reperfusion (IR) injury in rats. Methods Forty healthy male SD weighing 225-275 g were randomly assigned into 5 groups ( n = 8 each): group I sham operation (group S) ; group II intestinal IR (group IIR); group Ⅲ ischemic preconditioning (group Ipr); group IV ischemic postconditioning (group Ipo); group V Ipr+ Ipo. The rats were anesthetized with intraperitonel 20% urethane 5 ml/kg. Superior mesenteric artery (SMA) was occluded for 60 min followed by 60 min reperfusion. In group S, SMA was isolated but not occluded. In group Ipr, SMA was occluded for 10 min followed by 10 min reperfusion, and the rest procedures were performed using the method described in group IIR. In group Ipo, 60 min ischemia was followed by three 30 s episodes of ischemia at 30 s intervals for reperfusion. In group Ipr+ Ipo, Ipr was performed followed by Ipo and the procedures were performed using the methods described in group Ipr and Ipo. The animals were killed at 60 min of reperfusion. The intestinal tissues were immediately removed for determination of MDA content, SOD and MPO activities and the degree of damage to intestinal mucous membrane was scored according to Chiu score. Arterial blood samples were taken for determination of plasma concentrations of TNF-α and 1L-6. Results Compared with group S, Chiu score, MDA content, MPO activity, and plasma concentrations of TNF-α and IL-6 were significantly increased, whereas SOD activity decreased in the other 4 groups ( P < 0.05). Chiu score, MDA content, MPO activity, and plasma concentrations of TNF-α and IL-6 were significantly decreased, whereas SOD activity increased in group Ipr, Ipo and Ipr + Ipo as compared with group IIR ( P < 0.05). Chiu score and MDA content were significantly lower, whereas SOD activity higher in group Ipr + Ipo than in group Ipr and Ipo ( P < 0.05). No significant differences were detected in the indices between group Ipr and group Ipo ( P > 0.05). Conclusion Ischemic preconditioning-postconditioning can attenuate the intestinal IR injury in rats, and the efficacy is better than that of either Ipr or Ipo alone.     

Ultrastructural Observation of Protective Effect of Sini Decoction（四逆汤）on Ischemic Myocardium

ＵｌｔｒａｓｔｒｕｃｔｕｒａｌＯｂｓｅｒｖａｔｉｏｎｏｆＰｒｏｔｅｃｔｉｖｅＥｆｆｅｃｔｏｆＳｉｎｉＤｅｃｏｃｔｉｏｎ（四逆汤）ｏｎＩｓｃｈｅｍｉｃＭｙｏｃａｒｄｉｕｍＷＵＷｅｉ－Ｋａｎｇ（吴伟康）；ＹＡＮＧＫｕｎ（杨昆）；ＷＵＪｉｎ－ｌａｎｇ（吴金...     

四逆汤对动脉粥样硬化家兔脂代谢及血管内皮功能的影响

目的观察四逆汤预防性用药对家兔实验性动脉粥样硬化（ＡＳ）脂代谢及血管内皮功能的影响,探讨四逆汤的抗ＡＳ作用及可能机制。方法运用图象分析法测定主动脉内膜脂质斑块面积百分比。脂代谢各指标分别运用酶动力学法、一步法、公式计算法、免疫透射比浊法等方法测定。血清一氧化氮（ＮＯ）及血浆内皮素（ＥＴ）分别用酶法及放射免疫法检测。结果四逆汤可明显缩小主动脉内膜脂质斑块面积,降低血清总胆固醇、甘油三脂、低密度脂蛋白－胆固醇、载脂蛋白Ｂ及血浆ＥＴ浓度,提高血清ＮＯ及载脂蛋白Ａ含量,四逆汤高剂量组效果为佳,呈一定的量效依赖关系。结论四逆汤具有较好的抗ＡＳ作用,其作用机制与调节脂代谢、保护血管内皮细胞功能等有关。     

四逆汤改善经皮冠状动脉成形术后心肌缺血和再灌注心律失常的临床研究

采用动态心电图对30例经皮冠状动脉成形术(PTCA)的病人进行术后监测,并检测血中超氧化物歧化酶(SOD)和丙二醛(MDA)浓度的自由基指标动态变化。结果显示:PTCA术服用四逆汤的术后心肌缺血程度和再灌注心律失常发生率比单纯PTCA术更为显著地改善(p<0.05),其机制在于四逆汤能升高SOD活性和降低MDA含量,减轻PTCA术后心肌缺血再灌注损伤。     

大防风汤颗粒剂质量控制的初步研究

研究目的是确定大防风汤颗粒剂的质量控制方法。采用TLC法对研制的大防风汤颗粒剂和国外的大防风汤颗粒剂中黄芪、苍术、白芍及牛膝进行定性鉴定 ,利用Rp HPLC法测定颗粒剂中乌头碱的含量。结果 :乌头碱在 0 2～ 1 5 μg范围内具有良好的线性关系 ,回收率平均值为 97 92 % ,RSD为 1 88%。结论 :研制的大防风汤颗粒剂的质量控制方法简便、可靠 ,重现性良好 ,可有效地控制颗粒剂的质量 ,而国外的大防风汤颗粒剂不适用于此方法进行质量控制。     

保心胶囊对急性心肌缺血犬一氧化氮和内皮素的影响

保心胶囊对急性心肌缺血犬一氧化氮和内皮素的影响     

消栓口服液对球囊扩张损伤的兔主动脉壁的影响

探讨消栓口服液在主动脉球囊扩张损伤后对血管壁细胞的影响。新西兰雄性兔２０只均施行主动脉球囊扩张术。消栓口服液灌服２．５ｍｌ＝ｋｇ，每周６次。结果：１．术后主动脉内膜厚度和平滑肌细胞的＾３Ｈ－脱氧胸苷掺入率，用药组明显代于对照。２．术后主动脉内以血小板源生长因子体ｍＲＮＡ表达的组间比较，显示用药组有明显的抑制作用。     

四逆汤对多器官功能障碍综合征大鼠炎症介质和氧化应激的影响

目的 建立大鼠多器官功能障碍综合征(MODS)模型,观察四逆汤(SND)对MODS大鼠炎症介质和氧化应激的影响.探讨SND对MODS器官损伤的保护作用.方法 通过可逆性失血性休克以及静脉注射脂多糖两次打击以建立大鼠MODS模型,将大鼠随机分为空白对照组、MODS模型组和SND治疗组.采用常规病理检查,快速抽取主动脉血作血气分析和心、肝、肾功能有关酶学指标的检测,测定血清SOD、NO含量,以及ELISA法测定血清TNF-α和IL-6的含量.结果 病理检查显示模型组大鼠造模1 d后各重要脏器都有全身炎症反应综合征表现,3 d后更明显,而SND治疗组病变明显减轻;心、肝、肾功能生化指标在模型组明显升高(P<0.01),而治疗组较模型组有显著改善(P<0.05或P<0.01);与空白对照组比较,模型组大鼠血清SOD水平显著下降而NO水平显著升高,而治疗组除灌胃1 d后血清NO水平仍显著高于空白对照组(P<0.05)外,血清SOD和NO水平与对照组相比无显著差异;模型组和治疗组大鼠血清TNF-α和IL-6含量与空白对照组比较显著升高(P<0.05或P<0.01),且治疗组大鼠治疗3 d后血清TNF-α含量显著低于相应时间的模型组(P<0.05),治疗组大鼠治疗1 d后血清IL-6含量亦显著低于模型组(P<0.05).结论 四逆汤能有效防治MODS的发生和发展,其机制可能与其下调过度的炎症反应、提高机体的抗氧化能力以及减轻自由基损伤有关.