HO-1系统在器官移植中的细胞保护作用

血红素加氧酶-1是血红素分解代谢的关键酶。研究发现,HO-1过表达能改善移植器官再灌注后的功能恢复,延长移植物生存时间。本文综述了HO-1系统在器官移植中的细胞保护作用机制及应用前景。     

小剂量阿托品治疗急性重度有机磷中毒207例临床疗效分析

目的探讨应用小剂量阿托品治疗急性有机磷农药中毒的临床疗效。方法该研究对我院2006年3月至2010年2月确诊的急性重度有机磷农药中毒患者病历资料进行回顾性分析,对照组为进行常规治疗的确诊患者,治疗组为应用小剂量阿托品进行治疗的确诊患者。对二组患者治疗结果进行临床分析。二组间均数比较采用方差分析。计数资料采用四格表x2检验。结果该研究入选患者207例,二组患者年龄、性别及服药剂量分布差异无统计学意义（P〉0.05）。治疗组存活率较对照组明显提高,住院时间明显缩短,发生呼吸衰竭等并发症较对照组明显减少。结论小剂量阿托品治疗急性重度有机磷农药中毒疗效明显优于传统剂量阿托品治疗。     

我国肝移植的现状,问题及对策

我国肝移植工作起步晚、发展慢，目前又处于停滞状态，导致这一局面的主要原因在于传统文化的器官损献和接受器官移植的阻碍，死亡标准对高质量供肝的限制。移植伦理学不成熟，肝移植费用过高和对移移植组织不力。对此，可通过广泛宣传教育，尽推行脑死亡概念，有针对性地解决移植伦理学问题，合理利用现有医资源，并由国家统一组织’领导，实施肝移植工作，从而使肝移植尽早造福国民。     

急性有机磷中毒并发脑出血3例诊治体会

急性有机磷中毒并发脑出血(CH)临床少见,且易造成漏诊或误诊、误治.(?)5月以来共收治3例,报告如下.例1.男性,62岁.因口服(?)100ml 30min来诊.查体:血压19/10kPa,神志不清,呼吸困难,口吐白沫,口唇紫钳,皮肤潮湿,瞳孔小如针尖,心率61次/min,双肺布满湿罗音.查血胆碱酯酶活力5~u(试纸法·正常>70~u).给予清水洗胃、阿托品、解磷定静注、解磷定静滴等治疗.1h后患者出现阿托品化,但神志仍未转清.查体见左侧鼻唇沟变浅,右侧肢体肌张力增高,左侧肢体肌张力明显减低,病理反射未引出.考虑并发脑血管意外,行颅脑CT示:右侧基底节区出血.立即给予20%甘露醇250ml、脑活     

直肠异物处理体会

直肠异物处理体会西安医科大学第一附属医院（７１００６１）吕毅，曹思之，杨继恒，潘承恩直肠异物较少见，多数小异物可经正常排便或服泄剂后自肛门自行排出。较复杂的大异物常引起肛门直汤不同程度损伤或低泣肠梗阻．往往需外科手术处理。我院自１９５７～１９９１年共...     

Construction of Porcine CCK pDNA and Its Expression in COS-7 Cells

CCK correlates with the generation and progression of pancreatic cancer. The research aims to construct eukaryotic expression plasmid pIRES2-EGFP/CCK (CCK pDNA) and transiently express it in COS-7 cells. Total RNA was extracted from porcine intestinal mucosa. RT-PCR was used to amplify the aimed segments CCKcDNA which was then digested with EcoR1 and BamH1 and inserted into a eukaryotic expression plasmid pIRES2-EGFP to construct CCK pDNA. The con- structed plasmid was transfected into COS-7 cells by lepofectamineTM2000-mediated transfer method. The expression of CCK in transfected COS-7 cells was detected 24, 48 and 72 h post-transfection with fluorescence microscopy and the expression level of CCK mRNA in transfected COS-7 cells was assayed by using RT-PCR. The results showed CCK pDNA was successfully constructed and expressed transiently in COS-7 cells. Green fluorescent protein could be detected in the COS-7 cells transfected with porcine CCK pDNA 24 h post-transfection. At 48th h post-transfection, the number of positive cells was increased significantly and much brighter green fluorescence could be detected. And 72 h post-transfection, the green fluorescence of positive cells became even stronger, while no green fluorescence was detected in the control group. The expression of CCK mRNA in the cells was detectable by using RT-PCR. In COS-7 cells transfected with CCK pDNA a high level of porcine CCK mRNA was detected while no expression of porcine CCKmRNA was found in the cells trans- fected with null plasmid. It was concluded CCK pDNA was expressed successfully in COS-7 cells, which lays a foundation for further research on the relationship between CCK and tumor.     

硝基苯对小鼠脾细胞及巨噬细胞分泌NO的影响

目的研究硝基苯（NB）对小鼠脾细胞及巨噬细胞分泌NO的影响。方法选择3个染毒剂量即2、20、200mg／kg，每日灌胃1次，连续21d后，脱臼处死，观察NB对小鼠脏器系数、脾细胞和巨噬细胞NO分泌量的影响。结果随NB剂量的增大，小鼠巨噬细胞和脾细胞分泌NO的水平明显下降。结论在该实验条件下，NB可抑制小鼠脾细胞和巨噬细胞NO的分泌。     

育龄妇女叶酸代谢相关基因多态性分布研究

目的 了解育龄妇女叶酸代谢相关基因多态性的分布状况,利用基因检测技术筛查出叶酸利用能力差的高风险人群,为育龄妇女提供科学合理的叶酸补充方案.方法 选取144例育龄妇女作为研究对象,通过DNA提取、PCR扩增、PCR产物基因测序等分子生物学技术对亚甲基四氢叶酸还原酶(MTHFR)和甲硫氨酸合成酶还原酶(MTRR)等叶酸代谢相关基因进行单核苷酸多态性(Single Nucleotide Polymorphisms,SNP)位点的检测.结果 MTHFR677位点CC、CT、TT基因型频率分别为21.5%、40.3%、38.2%;MTHFR 1298位点AA、AC、CC基因型的频率分别为75.0%、22.2%、2.8%;MTRR66位点AA、AG、GG基因型频率分别为63.2%、31.3%、5.6%.结论 通过对MTHFR基因和MTRR基因相关位点的检测,可筛查出叶酸利用能力差的高风险人群,为其合理增补叶酸提供科学依据.