磷脂酰肌醇-3-激酶/蛋白激酶B和丝裂原活化蛋白激酶/细胞信号调节激酶1/2信号通路抑制剂对乳腺癌细胞缺氧诱导因子-2α表达的影响

目的 探讨磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)和丝裂原活化蛋白激酶/细胞信号调节激酶1/2(MEK/ERK1/2)信号通路抑制剂对缺氧诱导的乳腺癌MCF-7细胞中缺氧诱导因子-2α(HIF-2α)表达的影响.方法 乳腺癌MCF-7细胞常规培养24 h后,分为常氧组、缺氧组、缺氧+低剂量LY294002组...     

Treatment of Patients with Erectile Dysfunction by Shugan Yiyang Capsule(疏肝益阳胶囊):A Multi-Centered Randomized Controlled Trial

Objective: To evaluate the efficacy and safety of Shugan Yiyang Capsule (SGYY, 疏肝益阳ficiency Syndrome type with or without blood stasis. Methods: A multi-centered, randomized, double-blinded, placebo-controlled trial in 304 patients was conducted by dividing them into 3 groups, who received re101), 1.0g taken three times a day for 4 weeks. Besides, 205 patients were arranged in an open-label studyand treated with SGYY 1.0 g taken three times a day for 4 weeks. The total effective rate, total remarkablyeffective rate, erection recovery rate tested by penis hardness test ring, time taken for improving erection among groups, as well as therapeutic effect between psychogenic ED group and mild arteriogenic ED group were compared. Adverse reactions related to SGYY were recorded. And a 3-month follow-up study was conducted on 105 cured patients. Results: The trial was completed in 500 patients. The total effective rate and total remarkably effective rate in the SGYY treated double-blinded group were 88.0% and 64.0% respectively, and those in the open-label group 90.5% and 65.0%, respectively, all significantly higher th an thosein the placebo (21.0%, 6.0%) and SYBS groups (60.0%, 29.0%), P＜0.01. At the same time, the e rection recovery rate and improving time in SGYY group were significantly better than those in other groups ( P＜0.01). No adverse reactions related to SGYY were found. The successful rate of potency to have intercourse within 3 months was over 80% (100 patients out of the 105 cured patients). Conclusion: SGYY was an effective and safe treatment for patients with psychogenic and mild arteriogenic ED of Gan stagnation and Shen deficiency Syndrome type with or without blood stasis, especially for psychogenic ED patients.     

147例辐照血临床疗效观察

目的：观察输注γ射线辐照血预防输血相关移植物抗宿主病（TA-GVHD）。方法：观察干细胞移植术后、恶性血液病化疗抑制期,肾移植术后患者输注经γ射线辐照的红细胞悬液、浓缩血小板共147例,比较输血前后的血红蛋白（Hb),红细胞计数（RBC）和血小板计数（PLT）,观察输注后1个月有无皮肤、骨髓,肝肠等系统器官受损表现,结果：辐照血液成分输注后,Hb、RBC、PLT均明显升高,有显著性差异。所有患者无1例出现与输血相关的脏器受损表现。结论：γ射线辐照血可以有效预防TA-GVHD,可以达到与输注未经辐照的等量血液成分同样的疗效。     

直序商陆炮制品毒性成分测定

目的：探讨炮制对直序商陆毒性成分的影响。方法：采用薄层扫描法测定直序商陆不同炮制饮片中商陆毒素的含量。结果：清蒸品、醋煮品及醋品中商陆毒素的含量仅分别为原药材的１６．;７３％、２１．８８％及４５．８５％。结论：清蒸法和醋煮法为直序商陆炮制的最佳工艺。     

衣原体感染与男性不育

衣原体感染与男性不育南京医科大学组胚教研室（南京２１００２９）周亚东葛百明吴瑞娟祝辉江苏省中医院男性科翟亚春何映徐福松男性生殖道沙眼衣原体（Ｃｈｌａｍｙｄｉａｔｒａｃｈｏ－ｍａｔｉｓ，ＣＴ）感染对男性生殖功能的影响已引起人们的重视；ＣＴ是生殖道上皮...     

HBeAg阳性慢性乙型肝炎患者HBV基因型和亚型与肿瘤坏死因子α的关系

目的探讨福建部分地区HBeAg阳性慢性乙型肝炎(CHB)患者HBV基因型、亚型的分布与血清肿瘤坏死因子α(TNF-α)水平及其基因启动子区-238、-308位点基因多态性的关系。方法选取福建部分地区203例HBeAg阳性CHB患者,采用HBV基因分型(B、C基因型)荧光定量PCR法检测HBV基因型或HBVS基因为直接测序法检测HBV基因型、亚型;聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)的方法检测TNF-α-238及-308位点基因多态性,酶联免疫吸附试验(ELISA)测定血清TNF-α水平,并对结果进行统计分析。结果 HBV基因型B、C分别占58.6%、41.4%,其中150例HBVS基因为直接测序,Ba亚型81例,Ce亚型69例;B基因型患者平均年龄和体重指数(BMI)小于C型(P=0.020)。HBV基因型B、C的TNF-α水平、TNF-α-238和-308位点基因多态性的分布无统计学差异(P0.05)。结论福建部分地区HBeAg阳性CHB患者HBV基因型以B型为主,其次是C型,仅见Ba和Ce亚型;B基因型患者较年轻,BMI小于C型;但HBV基因型B、C与TNF-α水平、TNF-α-238和-308位点基因多态性可能无关。     

脉冲式皮下注射黄体生成素释放激素治疗男性促性腺激素缺乏症(附14例报道)

目的　观察脉冲式黄体生成素释放激素 (LHRH)皮下注射对男性促性腺激素缺乏症的治疗效果。　方法　给予知情同意的男性促性腺激素缺乏症 14例患者LHRH脉冲式腹壁皮下注射 ,每 90min 1个脉冲 (每个脉冲 10 μg) ,治疗 3～≥ 12个月。　结果　除 2例无效外 ,余 12例 (86% )有效 ,表现为青春期发育加速 ,体力增强 ,睾丸明显增大 ,阴茎增长 ,阴毛和腋毛生长。 5例有遗精现象 ,2例精液常规中有精子生成 ,所有患者在治疗期间性激素水平改善。　结论　脉冲式LHRH皮下注射是符合生理的替代治疗 ,是对男性特发性促性腺激素缺乏症可取的治疗方式。     

脑囊尾蚴病神经影像学表现与循环抗原相关性研究

目的探讨脑囊虫病血清循环抗原（CAg）与神经影像学（CT、MRI）的关系。方法根据诊断脑囊虫病的四个标准,对确诊的36例脑囊虫病病人行CAg定量测定及神经影像学检查并分期。结果根据神经影像学检查,36例脑囊虫病人在活虫期、变性死亡早期、变性死亡后期及钙化期之间的CAg水平有显著性差异（P〈0.01）。相关性分析表明血清中CAg的含量与脑囊虫病的神经影像学表现具有正相关性,其相关系数为0.871。结论（1）脑囊虫病人循环抗原与神经影像学（CT、MRI）表现具有相关性;（2）血清循环抗原的检查可用于对脑囊虫病的早期诊断和指导临床治疗。     

肺结核并肺癌25例临床讨论

近几年随着肺结核与肺癌单病种患者的增多,二病并存发病率亦呈上升趋势。由于二者在临床表现、影像学上有相似之处,且多以咳嗽、咳痰或伴发热、血痰、消瘦等为主要表现,故临床医生常常考虑一种疾病而忽略并存的另一种疾病,致漏诊。我院2005年8月～2008年8月住院