受微波高频辐射人员外周血5—羟色胺含量的变化

The relationship between 5-HT contents in peripheral blood and power density among workers exposed to microwave or high frequency radiation in the workshops were studied. At the same time, systems and disorder of central nervous system and cardiovascular system were examined. The results of study showed that the average 5-HT content in whole blood for high frequency radiation exposure group was lower than that of the control, but without statistical significance. Value for the microwave exposure group was significantly lower than that of the control (P less than 0.01). The 5-HT content of mechanical maintainers exposed to microwave of high frequency radiation were all lower than the contents of their corresponding control groups (P less than 0.01). But for the mechanic operators as compared with their corresponding control, no significant difference was found (P greater than 0.05). The power density exposed by the maintainers of both tested groups were all higher than that of the operators. The 5-HT contents in the whole blood were inversely proportional to the power density of microwave and high frequency groups. The incidence of neurasthenic syndrome, unsymmetric skin temperature in both limbs and hypotension was higher in the microwave and high frequency radiation groups than that in the control group. The incidence of bradycardia and some abnormal items of electrocardiograph in the microwave group was obviously greater than that in high frequency group, which may be related to different frequency exposed for these two exposure groups. Whether it is related to the contents of 5-HT needs further investigation.     

心神经调节不经中枢神经系的反射通路的研究

1 传统认为调节心功能的神经通路均经过中枢神经系脊髓和/或延髓,其一级感觉神经元位于脊神经节和迷走神经节.已知消化管的肠神经系统内有感觉神经元,胃肠有不经过中枢神经系的反射路径调节其功能.     

miR-409-3p靶向癌基因ELF2抑制非小细胞肺癌细胞系A549增殖

目的观察miR-409-3p对非小细胞肺癌细胞系A549增殖的影响及其机制。方法对照组为常规条件下培养的转染阳性对照核苷酸的A549细胞寡核苷酸组。实验组在对照组的基础上采用化学合成miR-409-3p模拟物,脂质体转染构建miR-409-3p高表达A549细胞;经q PCR检测,观察2组48 h后miR-409-3p的表达; MTT法检测细胞增殖;流式细胞计量术检测细胞凋亡和周期的差异。结果 miR-409-3p模拟物转染后,A549细胞中miR-409-3p表达水平显著升高(P0. 05);细胞总凋亡比例为22. 66%±4. 61%,显著高于对照组的7. 78%±1. 95%(P0. 05); G0/G1期为40. 21%±5. 37%,显著低于对照组细胞56. 09%±5. 22%(P0. 05);双荧光报告系统表明癌基因ELF2是miR-409-3p的靶基因,过表达miR-409-3p模拟物后,内源性ELF2蛋白表达水平显著下降(P0. 05)。结论 miR-409-3p可能通过调控ELF2的转录水平抑制非小细胞肺癌细胞A549增殖并促进其凋亡。     

云南省澜沧江下游地区虫媒病毒研究状况

澜沧江下游地区位于我省南部，包括思茅和西双版纳两个地州。根据我国气候带划分，该地区属边缘热带和南亚热带，具有海拔低、气温高、湿度大、雨量多以及众多生物群落和虫媒传染病复杂的特点。由于澜沧江-湄公河次区域开发已列为我国与东南亚各国合作项目。掌握该地区虫媒病毒的分布情况和宿主媒介特点，为疾病防治提供科学依据，对搞好大湄公河次区域开发有重要意义。近10多年来，我们在该地区采集到节肢动物、患者和健康人血清，以及动物内脏组织、血清标本共59988份（只），其中雌性成蚊8属44种54211只，蠓类950只、蜱类402只，虱子402只，鼠肺881份，蝙蝠脑453份，壁虎375只。患脑炎的乳猪脑组织1份，发热和脑炎患者血清143份，健康人血清1250份，蝙蝠血清291份，猴血清204份，猪血清197份，鼠血清535份。现将这些标本的病毒学检测和流行病学调查分析结果综述如下。     

2型糖尿病患者外周血CD146检测的临床意义

目的 探讨外周血CD146水平在诊断2型糖尿病患者肾损伤中的临床意义.方法 检测58例2型糖尿病患者和20例正常对照组外周血CD146(ELISA法)和U-MAlb(散射比浊法)的水平.结果 2型糖尿病患者肾损伤后外周血CD146、U-MAlb水平显著高于对照组,尿蛋白定性阳性组CD146与U-MAlb呈正相关.结论 2型糖尿病患者肾损伤后外周血CD146水平显著升高.     

深低温体外循环对犬趾蹼微循环指标的研究

本研究表明,深低温体外循环时,犬趾蹼微循环发生的变化与温度有关。平均动脉压(MAP)的变化不能反映组织灌注的优劣情况。毛细血管内的血流速度与灌注流量的高低有关。     

抗轮状病毒单克隆抗体简报

本研究用牛轮状病毒NCDV株为抗原,免疫2月龄Balb/c雌鼠,二个半月后取小鼠脾细胞与小鼠骨髓瘤细胞SP_2/o融合,共融合二次,融合率达100％。平行采用酶联免疫吸附试验和血凝抑制试验两种方法,从192个原始孔中初步筛选出12个阳性孔,其中11个孔为酶联免疫吸附试验阳性、1个孔为血凝抑制阳性。将其中几个孔进行三次有限稀释克隆化,得到3株抗轮状病毒单克隆抗体(2G_2、3F_4、4B_5)。在酶联免疫吸附试验中,2G_2株     

新洁尔灭对小白鼠抗早孕的病理组织学观察

本文报告用不同剂量的新洁尔灭和同体积生理盐水注入怀孕5天小白鼠的宫腔内,3天后杀死,观察其蜕膜、胚泡、宫内膜腺体和卵巢黄体病理学改变。实验表明:对照组无改变。实验组小白鼠的蜕膜细胞全部退变坏死。病变随着给药量的递增而渐趋严重;同时胚泡亦有退变和坏死。提示:新洁尔灭对早期妊娠小白鼠是有抗早孕效果的。根据实验结果,我们认为新洁尔灭对小白鼠抗早孕的机制,很可能是一个综合因素,有直接的,也有间接的。作者强调在抗早孕机制诸因素中,蜕膜退变坏死是一个重要的因素。