健脑益智汤对类阿尔茨海默病大鼠血清胆碱酯酶和脑皮质细胞凋亡的影响

目的:观察健脑益智汤对阿尔茨海默病(AD)大鼠血清乙酰胆碱酯酶(AChE)和脑皮质细胞凋亡的影响。方法:老年Wistar大鼠侧脑室注射聚集态的β-淀粉样蛋白25-35(Aβ_(25-35))建立复合式类AD大鼠模型,测定健脑益智汤灌胃给予AD大鼠(10.6,21.2,42.4g·kg~(-1),qd)3周后血清AChE和脑皮质细胞凋亡百分率,实验设立盐酸多奈哌齐(0.525 mg·kg~(-1))作为阳性对照组。结果:与正常对照组比较,AD模型组血清AChE活性增强(P<0.01),脑皮质细胞凋亡百分率显著增加(P<0.01);与模型组比较,健脑益智汤中、高剂量组AChE活性和脑皮质细胞凋亡百分率均降低(P<0.05,P<0.01),与盐酸多奈哌齐组接近。结论:健脑益智汤可抑制AD大鼠血清Ache活性和脑皮质细胞凋亡。     

人类疾病基因工程动物模型的研究与应用

人类疾病动物模型是研究疾病发生机制、确定治疗方案及药物研究的重要载体,在人类医学发展史中占有十分重要的地位。基因工程动物模型作为21世纪人类研究疾病的理想动物模型,在生命科学的各个领域得到了广泛重视和研究。本文就转基因动物模型和基因剔除动物模型在医学和药学方面的应用研究予以综述。     

近交系C57小鼠与封闭群昆明小鼠超排卵数的比较

目的 观察C5786小鼠(近交系)和昆明小鼠(封闭群)超排卵后卵母细胞数量的差别.方法 分别对4周龄和7周龄C5786小鼠与昆明小鼠注射孕马血清促性腺激素(PMSG)和人绒毛膜促性腺激素(HCG),进行超排卵处理,观察小鼠超排卵母细胞数量.结果 超排卵母细胞数量为:4周龄昆明小鼠(16.2±2.5)个/只,7周龄(25.2 ±3.1)个/只;4周龄C5786小鼠(9.8±1.2)个/只,7周龄C5786小鼠(18.1±2.2)个/只.超排卵母细胞数量在同种小鼠不同周龄间和同周龄不同小鼠间比较,均有统计学差异(P<0.05).结论 昆明小鼠超排卵母细胞数量高于C5786小鼠.     

五味子乙素对实验性矽肺大鼠肺组织MMP-2和TIMP-1蛋白含量的影响

目的:观察五味子乙素(Sch-B)对二氧化硅致大鼠矽肺中基质金属蛋白酶2(matrix metallopro-teinase,MMP-2)和其抑制物基质金属蛋白酶组织抑制因子1(tissue inhibitor of matrix metalloproteinase,TIMP-1)含量的影响。方法:96只大鼠随机分为对照组、染尘组和Sch-B干预组,每组32只,采用暴露式气管内注入SiO2混悬液法建立大鼠矽肺模型,造模后1 d灌胃给予Sch-B干预治疗,分别在药物干预3,7,14,28 d时处死大鼠,取其肺组织,HE染色检测肺组织病理改变;ELISA法测定大鼠肺组织中MMP-2和TIMP-1蛋白的含量。结果:与对照组相比,染尘组MMP-2和TIMP-1蛋白含量在3,7,14,28 d时明显增高(P<0.01);与染尘组相比,Sch-B干预组MMP-2和TIMP-1蛋白含量均明显降低(P<0.01),但仍显著高于对照组(P<0.01),差异有统计学意义。结论:Sch-B对二氧化硅致大鼠矽肺中不同时间点肺组织MMP-2和TIMP-1的表达均有一定程度的抑制作用,能在一定程度上调整MMP-2和TIMP-1的比值使其趋于平衡,具有减轻二氧化硅致矽肺大鼠肺纤维化的作用。     

中国地鼠山医群体近交系E繁殖性状的遗传力估计

目的 对中国地鼠山医群体近交系E的一些繁殖性状进行遗传力估计分析.方法 采用平均信息约束最大似然法(AIREML),利用WOMBAT软件进行处理.结果 产仔数、离乳数、胎间隔2-1和胎间隔3-2的遗传力均较低,分别为0.05、0.096、0.182和0.116.结论 中国地鼠山医群体近交系E繁殖性状的遗传力均属于低遗传力.     

射频靶点热凝椎间盘修复术治疗椎间盘突出症200例

目的:探讨射频靶点热凝纤维环修复术一次性治疗椎间盘突出症这一技术的临床实用性和术中热凝靶点定位。方法:根据临床表现及MRI或/和CT片确定射频热凝的靶点位置和热凝周期,同时设计术中患者体位和穿刺途径。结果:200例优178例占89%,良10例,可4例,差8例,优良率94%,有效率96%。结论:射频靶点热凝纤维环修复术是治疗椎间盘突出症一种安全,微创、优良率高的治疗方法。     

川崎病21例临床分析

本文21例川崎病分析揭示:川崎病以持续发热5日以上,结膜充血,口咽变化,手足硬肿,掌跖发红及恢复期指端膜状脱皮,躯干部多形红斑及颈淋巴结肿大为主要特征。它是一种以全身血管炎为主要病变的急性发热性疾病,常侵犯冠状动脉引起不同程度的病理改变;治疗以抗凝药物阿斯匹林为主。     

Effect of schisandrin B on lung mRNA expression of transforming growth factor-β1 signal transduction molecule in rat lungs exposed to silica

Objective To investigate the effects of schisandrin B (Sch-B) on expression of transforming growth factor-β1 (TGF-β1) and signal transduction molecule mRNA in rat lungs exposed to SiO2,and explore the intervention mechanism of Sch-B on pulmonary fibrosis induced by SiO2. Methods Ninety six Wistar rats were randomly divided into control (normal saline) group, SiO2 group and SiO2 plus Sch-B group.The rats were exposed to SiO2 by direct tracheal instillation to establish the silicotic animal models. SiO2 group and SiO2 plus Sch-B group were treated with 1ml SiO2 (50 mg/ml) for each rat From the first day after model establishment, SiO2 plus Sch-B group were orally given Sch-B (80 mg/kg) a day, control group and silica group were orally given olive oil. On the 3rd, 7th,14th and 28th days after treatment, 8 rats in each group were sacrificed and samples were collected. The histo-pathological examination of lung was performed by HE staining. The expression levels of TGF-β1 、TGFβR Ⅱ and Smad4 mRNA in the lung tissues were detected by RT-PCR. Results The results of histo-pathological examination showed that in SiO2 group, lung tissues were injured obviously; the alveolar inflammation with alveolus interval edema and inflammation cell infiltration appeared on the 3rd and 7th days; the alveolus interval became thicker, became thicker, fibroblast and collagen matiix increased markedly on 14th day; the alveolar structure was damaged, alveolar wall thickened obviously,collagen aggradation and pulmonary fibrosis displayed on 28th day. The alveolar inflammation and pulmonary fibrosis in SiO2 plus Sch-B group were significantly less than those in SiO2 group. The expressions levels of TGF-β1、TGFβR Ⅱ and Smad4 mRNA (TGF-β1: 1.03±0.31 、1.33±0.39、1.08±0.26、0.82±0.16,TGF-βR Ⅱ:0.65 ±0.11、0.80 ±0.16、0.83 ±0.24、0.62 ±0.15, Smad4: 0.87 ±0. 15、0.68 ±0.11、0.78 ±0.19、0.30 ±0.08) in SiO2group were significantly higher than those in the control group (TGF-β1:0.59±0.22、0.55 ±0.25、0.56±0.20、0.55 ±0.12,TGR-βR Ⅱ:0.28 ±0.13、0.31 ±0. 15、0.34 ±0.15、0.27 ±0.09,Smad4:0.23 ±0.11、0.40 ±0. 12、0.39 ±0.12、0.18±0.06)(P＜0.01 or P＜0.05), but the expression level of TGF-β1 mRNA was the highest on the 7th day. The expression levels of TGF-β1 and Smad4 mRNA (TGF-β1: 0.68 ±0.28、0.88 ±0.25、0.75 ±0.11、0.61 ±0. 14,Smad4:0.25 ±0.12、0.45 ±0.09、0.44 ±0.07、0.21 ±0.04) in SiO2 plus Sch-B group were significantly lower than those in SiO2 group (P＜0.01 or P＜0.05),but there were no significant differences of the TGFβR Ⅱ mRNA expression levels between SiO2 group and SiO2 plus Sch-B group. Conclusion Sch-B can reduce the pulmonary fibrosis induced by SiO2 through inhibition of the mRNA express of TGF-β1 and Smad4 in the lung tissue, modulating the TGF-β1/Smad4 signal transduction pathway and inhibiting the target gene activation.     

谈国家自然科学基金的申报

通过对９３～９７年我校国家自然科学基金未获资助因素分析，表明我校未获资助原因主要表现为研究目标不明确，研究内容重复；缺乏创新性；技术路线不当；立题依据不足等。加强信息采集，增加工作积累，发挥自身优势，加强合作，促进交叉，加强科研管理等是提高申报质量的必要措施。