EFFECT OF“764-1”EYEDROPS ON ULTRASTRUCTURE OF CORNEAL SCARS IN RABBITS

“764-1”eyedrops prepared by the Instituteof Hematology showed beneficial clinical effecton corneal scars.We set up a model of cornealscar in rabbits in order to study the mechanismof the“764-1”eyedrops' effects on the scarultrastructures in rabbits and we present ourfindings here.These indicated that the eyedropsexerted a regulatory action on the metabolismof the scar connective tissue.The principaleffect of the drug lay in its inhibition of theproliferation and function of collagen-producingcells.The collagen-producing cells in cornealscar underwent hyperplasia and hypertrophy,irregular in direction,and the collagen fibersof varying width were also in disorderly arran-gement.There were amorphous substancesaround the cells.Fibroblastoid reticular cellsand ordinary fibroblasts with developed roughendoplasmic reticulum were the two types ofcollagen-producing cells that proliferated in thecorneal scars.After treatment the number ofparenchymal cells was reduced.In appearancethe fibroblasts were slender and fusiform withlittle cytoplasm,clear cytoplasmic membranesand an increased amount of nuclear heterochro-matin.They lay parallel to the neatly alignedcollagen fibers.These fibroblasts tended togrow to maturity.The collagen fibers werenearly uniform in size and the laminae formedby them ran vertical to each other.The changein distribution of the collagens effected a changein the cornea from cloudy toward translucent.     

活血化瘀法治疗硬皮病临床实验观察

本文介绍应用活血化瘀法治疗各型硬皮病725例,分3组用不同的活血化瘀方剂治疗,取得了较好的疗效。1、2、3组系统性硬皮病显效率分别为28.8%、37.2%、40.7%,有效率分别为98.0%、97.7%、96.6%;局限性硬皮病显效率分别为47.6%、46.3。44.9%。临床与实验研究证明活血化瘀药物作用是多方面的,可改善血液循环与结缔组织代谢,特别是对结缔组织有软化作用。     

中药79—2片剂治疗硬皮病疗效观察

本文报道以中药79—2片剂治疗各型硬皮病414例,并进行了与增肌注射液各76例的随机对照。治疗后,系统性硬皮病118例,显效率为40.7%,总有效率为96.6%;局限性硬皮病296例,显效率为44.9%,总有效率为97.6%。随机对照组,79—2纽显效率为53.9%,总有效率为94.7%,增肌组显效率为27.6%,总有效率为78.9%,经统计学处理,P<0.01,有显著性差异。实验室检查证实了该药确有改善血液循环及胶原纤维代谢、调节纤维母细胞功能等作用。长期使用无副作用。     

人骨髓基质细胞中某些细胞因子和特异性标记的免疫电镜定位

为研究骨髓基质细胞支持造血的分子基础，应用胶体金和银增染的单标和双标记技术，对长期培养的人骨髓基质细胞（ＨＬＴＢＭＣ）中几种重要细胞因子（ＧＭ－ＣＳＦ、Ｇ－ＣＳＦ、ｂ－ＦＧＦ、ＩＬ－３）以及细胞特异性标记进行免疫电镜研究。结果表明ＧＭ－ＣＳＦ、Ｇ－ＣＳＦ和ｂ－ＦＧＦ可由长期培养的基质细胞产生。基质细胞主要以内皮细胞和成纤维细胞为主。ｖＷＦ（作为内皮细胞特异性标记），Ｆｂ－ＳＰ（成纤维细胞表面蛋白，主要标记成纤维细胞）及ＧＭ－ＣＳＦ、Ｇ－ＣＳＦ和ｂ－ＦＧＦ的双标记结果证明，这些细胞表面及细胞外基质中均可见大量细胞因子分布，且细胞外基质比细胞表面有更强的标记密度，证明细胞外基质有浓缩细胞因子的作用。这种模式可能有利于对造血细胞的支持。硫酸类肝素（ｈｅｐａｒａｎｓｕｌｐａｔｅ）和ＧＭＣＳＦ双标记结果显示两者共存于同一位点，证明生长因子是束缚于细胞表面和细胞外基质的硫酸类肝素中；提示骨髓基质细胞及其细胞外基质上各种生长因子和硫酸类肝素等质和量的变化对支持造血均十分重要。     

从湿热郁阻论治金实不鸣

金实不鸣历代医家多从风寒伤肺、闭阻喉窍论治,然临床观察湿热郁阻三焦亦可致金实不鸣。认为当从清利湿热,宣畅三焦以开音论治。     

胆囊后三角入路三孔法腹腔镜胆囊切除术

目的探讨胆囊后三角入路三孔法腹腔镜胆囊切除术(LC)的可行性。方法回顾分析25例胆囊后三角入路三孔法LC的临床资料,主要包括手术时间、出血量及手术并发症。结果 25例手术均获成功,无1例转四孔法,手术时间25～60 min,平均40 min;术中出血量1～10 ml,平均3 ml。全组无胆管损伤、胆漏、出血等严重并发症。25例随访4～13个月,平均7个月,无胆管狭窄及胆系症状。结论胆囊后三角入路有助于解剖和辨认Calot三角内的组织结构,可有效的减少术中出血和胆管损伤等并发症,胆囊后三角入路三孔法腹腔镜胆囊切除术是安全、可行的。