4种综合疗法治疗尖锐湿疣的效果比较

目的 观察4种综合治疗方法对不同特点的尖锐湿疣（CA）的疗效。方法 对155例经临床和（或）醋酸白试验和（或）病理确诊的CA患者根据临床损害特点随机分为4组，分别采用不同的综合治疗方案[二氧化碳（CO2）激光、干扰素α-2b、氟尿嘧啶、鬼臼毒索酊等]进行治疗，每2，4，8，12周观察复发情况，所有复发者，不论其复发次数多少均记1次复发。结果 155例患者中复发16例，总体复发率为10．3％。结论 对CA患者应针对不同的临床损害特点采用不同的综合治疗方案，这种方法可以降低复发率。     

Local Expression of Vaginal Th1 and Th2 Cytokines in Murine Vaginal Candidiasis under Different Immunity Conditions

To investigate the expression of vaginal Th1 and Th2 cytokines in rats with experimental vaginal candidiasis under different immune conditions, ICR murine vaginal candidiasis model was established and immno-suppressed murine models of vaginal cadidiasis were established in estrogen-treated mice. Non-estrogen-treated mice were used as controls. The mRNA level of Th1 （IL-2）/Th2 （IL-4, IL-10, TGF-β1） cytokines in murine vaginal tissues was determined by RT-PCR. The cykotine in local tissues was increased to different extent under normal immune condition. IL-2 mRNA was increased during early stage of infection, while IL-10 was increased transiently during late stage of infection. TGF-β1 production was found to be increased persistently. At same time, the expression of IL-2 mRNA was suppressed in immno-suppressed group, and the level of IL-4, IL-10, and TGF-β1 were higher than the normal immunity group to different degree during infection. The high level of IL-2 mRNA during early stage of infection was associated with clearance of mucosal Candidia albicans （C. albicans）, and its expression suppressed leading to decreased clearance of mucosal C. albican in immuno-suppression. The over-expression of IL-4 and IL-10 could significantly enhance the susceptibility to C. albicans infection in mice.     

Inhibition of Invasion and Up-regulation of E-cadherin Expression in Human Malignant Melanoma Cell Line A375 by（-）-Epigallocatechin-3-gallate

The inhibitory effects of （-）-epigallocatechin-3-gallate （EGCG） on the invasion of human malignant melanoma cell line A375 and the possible molecular mechanisms of this effect were investiaged. A375 cells were pretreated with 20 μg/mL EGCG for 24, 48 and 72 h respectively and the E-cadherin expression was detected by Western blot analysis. A375 cells were also pretreated with different concentrations of EGCG （1, 5, 10 and 20 μg/mL） for 72 h and the expression of E-cadherin was measured by RT-PCR. The adhesion and invasion of A375 cells were tested by cell-matrigel adhesion assay and matrigel invasion assay respectively. The results showed that EGCG could significantly up-regulate the expression of E-cadherin time-and concentration-dependently （both P〈0.05）. Statistical analysis showed that A375 cells invasion was inhibited by EGCG and correlated with the up-regulation of E-cadherin expression. It was suggested that EGCG strongly inhibited invasion of A375 cells, and the inhibition mechanism was possibly associated with the up-regulation of E-cadherin expression.     

Expression of Apoptosis Related Protein in Skin Lesions of Lichen Planus and Its Implication

In order to investigate the role of Caspase-3 and Bax in the pathogenesis of lichen planus, immunohistochemistry was used to detect the expression of Caspase-3 and Bax in skin lesions of the patients with lichen planus and skin tissues of normal subjects. The results showed that positive rate of Caspase-3 and Bax expression in lichen planus were significantly higher than that in normal skins （both P〈0.05）. Meanwhile, there was a obvious correlation between the increase of Caspase-3 and that of Bax in lichen planus. The expression of Caspase-3 and Bax might play an important role in the development of lichen planus.     

合并系统性红斑狼疮的尖锐湿疣患者细胞免疫功能检测

目的　研究合并系统性红斑狼疮 (SLE)的尖锐湿疣 (CA)患者细胞免疫功能状态。方法　运用流式细胞术 ,分析了 2 1例合并SLE的CA患者的外周血中T淋巴细胞免疫表型的特点。同时用ELISA法测定了血清中白介素 2(IL 2 )和白介素 12 (IL 12 )的水平变化。结果　 2 1例合并SLE的CA患者外周血中总T(CD3 + ) ,Th(CD4+ )细胞及Th/Ts(CD4+ /CD8+ )均低于正常对照组和单纯CA患者对照组 (P <0 .0 1或P <0 .0 5 ) ,而Ts(CD8+ )的比例显著高于正常对照组和单纯CA患者对照组 (P <0 .0 1)。 2 1例合并SLE的CA患者血清中IL 2和IL 12水平均低于正常对照组和单纯CA患者对照组 (P <0 .0 1)。结论　合并SLE的CA患者的细胞免疫功能受到不同程度的损害 ,并且较单纯CA患者的细胞免疫功能受到更严重的损害。     

Correlation between the Expression of Fas, Bcl-2 in Peripheral Blood Lymphocytes and the LeveL of IFN-γ,IL-4 in Serum of Patients with Condyloma Acuminata

Condylomaacuminata (CA)isakindofsexuallytransmitteddiseasecausedbyhumanpapillomavirus(HPV) .Theoccurrence ,progressandresolutionofCAiscloselyrelatedwithimmunologicalfunctionofbody ,especiallycellularimmunologicalfunctionofboby .Theimmunologicalfunctionoft…     

干扰素局部注射治疗扁平疣61例

目的观察干扰素局部注射治疗扁平疣的疗效与安全性。 方法将122例扁平疣患者随机分为两组各61例，治疗组给予重组人干扰素注射液α 2a，视皮疹数量用注射用水和2%利多卡因等量溶解稀释，于皮损内注射，每周1次；对照组口服阿昔洛韦片，每次0.2 g，qid，局部外用阿昔洛韦软膏，bid。两组均治疗28 d为1个疗程。共观察2个疗程，随访6个月。结果治疗组第1个疗程结束痊愈率为6.56%，第2个疗程结束痊愈率91.8%，均明显高于对照组（分别为1.64%和4.92%）（均P＜0.05）。两组均未出现严重不良反应。 结论干扰素局部注射治疗扁平疣疗效确切，方法简单，值得临床推广。     

解脲支原体对10种抗生素的体外敏感性研究

目的：研究解脲支原体（UU）对10种抗生素耐药情况,指导临床合理用药。方法：采用UU培养和药敏试剂盒测定UU的体外抗菌作用。结果：119例UU阳性总的耐药发生率为94．12％,耐2种或2种以上占69．75％,对10种抗生素耐药性高低依次为红霉素、四环素、美满霉素、乙酰螺旋霉素、强力霉素、氧氟沙星、罗红霉素、交沙霉素、阿齐霉素、左旋氧氟沙星。结论：UU耐药发生率高,并存在多重耐药。     

淋球菌对六种抗菌药物敏感性的测定

目的：观察淋球菌对6种常用抗菌药物的敏感性。方法：分离123株淋球菌,分别进行青霉素、四环素、大观霉素、环丙沙星、洛美沙星、头孢曲松的敏感性测定。结果：耐青霉素、四环素、环丙沙星、洛美沙星的菌株分别占56.9%,70.7%,22.8%,30.9%,耐大观霉素1株,未发现耐头孢曲松的菌株;青霉素、四环素、环丙沙星、洛美沙星之间存在交叉耐药及多重耐药（占14.6%)。结论：头孢曲松、大观霉素可作为武汉地区治疗淋病的首选药物,其余4种不作为首选。     

淋球菌mtr系统的IR区基因突变与其多重耐药性的关系

目的 探索多传递耐药(mtr)系统反向重复序列(IR)区基因突变与淋球菌染色体介导多重耐药性的关系。方法 琼脂稀释法做淋球菌药敏试验,选择耐不同药物的菌株,聚合酶链反应扩增包含IR区的目的基因,然后对扩增产物测序。结果 2株敏感株及5株仅耐青霉素菌株无IR区基因突变,17株多重耐药菌株中,1株同时耐青霉素和阿奇霉素的淋球菌有IR区碱基T/A,T/A插入,其余耐药株IR区均有碱基A/T缺失。结论 淋球菌染色体mtrR启动子区域的IR区基因突变会引起淋球菌多重耐药株的产生,增加淋球菌对抗生素的抗性。