自噬在非酒精性脂肪性肝病中的变化及作用

自噬是一种溶酶体依赖性降解途径,已有大量研究报道,自噬参与了非酒精性脂肪性肝病(NAFLD)的发生及发展.在NAFLD早期,自噬增强,并可以通过抑制引起NAFLD的“二次打击”延缓NAFLD的进展.在NAFLD晚期,由于自噬相关基因(Atg)7降解、哺乳动物雷帕霉素靶蛋白通路过度激活、高胰岛素血症、自噬-溶酶体蛋白水解功能减弱、自噬体膜及溶酶体膜脂质构成改变、肝细胞内钙离子水平增加引起自噬减弱,加重了NAFLD.     

小剂量MK-801对大鼠参照记忆、空间工作记忆和逆反学习能力的影响

目的 探讨腹腔注射N-甲基-D-天冬氨酸 (NMDA )受体拮抗剂地卓西平马来酸盐(MK-801)对大鼠认知功能的影响.方法 成年雄性SD大鼠随机分为MK-801组和对照组,分别腹腔注射小剂量生理盐水或MK-801(0.1 mg/kg)20 min后在Morris水迷宫中评定MK-801对大鼠参照记忆、空间工作记忆和逆反学习能力的影响.结果 参照记忆任务中,与对照组相比MK-801组逃避潜伏期延长(P<0.05),且在目标象限的探索时间百分比[(22.7±2.9)%]与相邻象限[(24.0±0.9)%]和对立象限[(29.3±2.4)%]的差异无统计学意义 (P>0.05);空间工作记忆任务中,对照组匹配试次潜伏期显著短于样本试次[(37.6±6.0) vs (61.5±6.3),P<0.05],而MK-801组两试次潜伏期差异无统计学意义[(53.8±7.8) vs (62.2±7.1),P>0.05];逆反学习任务中,与对照组相比MK-801组潜伏期明显延长(P<0.05),且在空间探索中新旧目标象限探索时间百分比差值小于对照组[(-0.01±4.7) vs (23.5±6.2),P<0.01].结论 腹腔注射小剂量MK-801破坏了大鼠的参照记忆、空间工作记忆和逆反学习,提示其在多个认知维度上可模拟精神分裂症患者的认知缺陷.     

母婴床旁护理模式在产科优质护理服务中的应用

目的:探讨母婴床旁护理模式在产科优质护理服务中的应用效果。方法:将2010年4月410例住院产妇作为对照组,应用传统母婴同室护理模式;将2010年5月410例住院产妇作为观察组,应用母婴床旁护理模式。结果:观察组产妇及家属对健康教育知识、新生儿护理方法的掌握及对护理工作满意度均明显高于对照组(P<0.05)。结论:母婴床旁护理模式可有效提高健康教育效果,改善护患关系,增强产妇育儿信心,更适应优质护理服务的要求。     

奥氮平对谷氨酸功能低下的精神分裂症小鼠模型的作用

Objective To investigate the effects of olanzapine on hyperlocomotion and deficient prepusle inhibition (PPI) of hypoglutamatergic schizophrenia model in mice produced by dizocilpine maleate (MK-801), an N-methyl-D-aspartate (NMDA)antagonist.Methods (1) To investigate the effects of olanzapine on explorative behavior and spontaneous activity, three doses of olanzapine ( 0.1, 0.2, 0.3 mg/kg, i.p.) or vehicle was injected 30 min before the test.Locomotor activity was recorded for 30 min with an automated video tracking system, in which the components of the locomotor activity were divided into exploration (the first 10 min) and spontaneous activity (the second 20 min).(2) To examine the effects of olanzapine on MK-801-induced hyperlocomotion, mice were administered with olanzapine (0.1, 0.2 and 0.3 mg/kg) or vehicle 5 min before administration of MK-801 (0.25 mg/kg).After the second injection, locomotor activity was recorded for 90 min by the video tracking system.( 3 ) To explore the effects of olanzapine on intact and MK-801-disrupted sensorimotor gating, olanzapine (0.3, 1,3 mg/kg, i.p.) or the vehicle were injected 35 min before the start of the experiment, MK-801 (0.5 mg/kg, i.p.) or the same volume of saline was administered 5 min before the PPI experiment.Results ( 1 ) Olanzapine (0.2 and 0.3 mg/kg) significantly inhibited the explorative behavior and spontaneous activity (P ＜ 0.05 ) .Olanzapine at 0.1 mg/kg did not affect exploration ( P = 0.363 ) and spontaneous activity ( P = 0.196 ).Olanzapine (0.1 - 0.3 mg/kg) dose-dependently antagonized MK-801 -induced hyperlocomotion.(2) None of the olanzapine doses tested had a significant effect on baseline PPI.Olanzapine ( 1 - 3 mg/kg) dosedependently restored the MK-801-induced deficits in PPI.Conclusion Olanzapine specifically inhibited the MK-801-induced hyperlocomotion and deficits in PPI in mice and the results are also consistent with clinical findings.     

奥氮平对谷氨酸功能低下的精神分裂症小鼠模型的作用

Objective To investigate the effects of olanzapine on hyperlocomotion and deficient prepusle inhibition (PPI) of hypoglutamatergic schizophrenia model in mice produced by dizocilpine maleate (MK-801), an N-methyl-D-aspartate (NMDA)antagonist.Methods (1) To investigate the effects of olanzapine on explorative behavior and spontaneous activity, three doses of olanzapine ( 0.1, 0.2, 0.3 mg/kg, i.p.) or vehicle was injected 30 min before the test.Locomotor activity was recorded for 30 min with an automated video tracking system, in which the components of the locomotor activity were divided into exploration (the first 10 min) and spontaneous activity (the second 20 min).(2) To examine the effects of olanzapine on MK-801-induced hyperlocomotion, mice were administered with olanzapine (0.1, 0.2 and 0.3 mg/kg) or vehicle 5 min before administration of MK-801 (0.25 mg/kg).After the second injection, locomotor activity was recorded for 90 min by the video tracking system.( 3 ) To explore the effects of olanzapine on intact and MK-801-disrupted sensorimotor gating, olanzapine (0.3, 1,3 mg/kg, i.p.) or the vehicle were injected 35 min before the start of the experiment, MK-801 (0.5 mg/kg, i.p.) or the same volume of saline was administered 5 min before the PPI experiment.Results ( 1 ) Olanzapine (0.2 and 0.3 mg/kg) significantly inhibited the explorative behavior and spontaneous activity (P ＜ 0.05 ) .Olanzapine at 0.1 mg/kg did not affect exploration ( P = 0.363 ) and spontaneous activity ( P = 0.196 ).Olanzapine (0.1 - 0.3 mg/kg) dose-dependently antagonized MK-801 -induced hyperlocomotion.(2) None of the olanzapine doses tested had a significant effect on baseline PPI.Olanzapine ( 1 - 3 mg/kg) dosedependently restored the MK-801-induced deficits in PPI.Conclusion Olanzapine specifically inhibited the MK-801-induced hyperlocomotion and deficits in PPI in mice and the results are also consistent with clinical findings.     

萘派地尔治疗女性顽固性下尿路症状100例分析

我们于2005-08～2006-03对女性下尿路症状患100例采用萘派地尔治疗，取得较好的效果，现总结如下。     

加强思想政治工作是做好办公室工作的重要保证

思想政治工作是我们党的优良传统。在当前改革开放的新形势下,思想政治工作在社会主义建设和一切经济工作中具有更为重要的地位和作用。作为学校中心枢纽的办公室,毫无疑问,也必须依靠思想政治工作来推动整个办公室工作的顺利开展。 几年来,我们结合办公室工作的特点,认真分析影响办公室人员工作积极性的各种因素积极主动地开展了思想政治工作,取得了一定的效果。     

舍尼通治疗慢性非细菌性前列腺炎的临床观察

舍尼通(prostat)作为一种植物制剂，已在国内外广泛用于治疗慢性前列腺炎和前列腺增生，并取得了一定的疗效。我院2003年5月-2004年5月应用舍尼通治疗50例非细菌性前列腺炎患者取得良好效果，现报道如下。     

浅谈做好学校办公室工作的几点认识

一所学校管理水平的优劣,在很大程度上取决于办公室的工作水平。因此,努力提高办公室工作水平,把办公室工作从经验性管理,从简单的办文办事转向科学化管理的轨道上来,是当前办公室工作的当务之急。本文仅就做好办公室工作谈几点认识。一、努力强化协调职能,保证学校各项工作的正常运转办公室作为学校领导的直接办事机构,它是各种信息输出和反馈的汇合点。上下左右各种文件要通过这