AMPKβ2对运动中骨骼肌细胞肌糖原的调节及机理研究

目的 通过饮食和运动的干预,观察大鼠骨骼肌细胞中肌糖原浓度的变化对AMPKβ2蛋白表达和定位的影响,从而进一步推断一次训练课和短期运动、肌糖原浓度变化对AMPKβ2基因表达的影响以及AMPKβ2对肌糖原的调节作用和机理.方法 成年雄性wistar大鼠60只,随机分为一次训练组25只,短期运动组35只.高糖组均给予高糖食物和5％蔗糖水饮用,低糖组均给予低糖食物和蒸馏水饮用;除安静组和控制组外,所有大鼠每天适应性游泳30分钟后,分别进行3天和8周的游泳训练.所有大鼠训练宰杀后取大鼠腓肠肌外侧白肌,采用西式印迹分析骨骼肌总蛋白和核蛋白中AMPKβ2的表达水平以及用硫酸蒽酮法测定骨骼肌糖原浓度的变化.结果 一次性训练组,骨骼肌细胞总蛋白中AMPKβ2的表达各组比较均没有变化,而在核蛋白中发现,肌糖原浓度显著减低,AMPKβ2的表达升高;短期运动组中,训练前后,低糖组骨骼肌细胞中AMPKβ2在总蛋白中表达升高,而高糖组没有变化,同时高糖组整体肌糖原浓度明显高于低糖组;肌糖原浓度降低的同时,骨骼肌中总蛋白中AMPKβ2表达没有变化,而核蛋白升高;负荷刺激组前后,骨骼肌细胞核蛋白中AMPKβ2表达都明显升高,总蛋白呈现减少趋势,同时肌糖原浓度均降低.结论 短期运动中,骨骼肌细胞内肌糖原浓度化与骨骼肌细胞内AMPKβ2蛋白表达有密切关系,低浓度肌糖原促进AMPKβ2蛋白表达,高浓度肌糖原抑制AMPKβ2蛋白表达;无论在高肌糖原还是在低肌糖原以及低肌糖原转高肌糖原模型中,都发现骨骼肌细胞中AMPKβ2定位变化都对应着肌糖原浓度变化,低肌糖原有利于骨骼肌细胞中AMPKβ2的转位,高肌糖原则不利于其转位.     

环抱式肋骨接骨器内固定医源性断肋对术后疼痛的影响

目的 观察医源性断肋后应用环抱式肋骨接骨器一期修复内固定对术后疼痛的影响.方法 肺癌、食管癌及脓胸患者96例,需要进行开胸、断肋手术.按住院单双号分为两组,A组48例应用环抱式肋骨接骨器一期修复内固定肋骨,B组48例应用传统的双7号丝线直接固定肋骨,比较两组患者术后疼痛程度.结果 A组术后疼痛程度轻于B组(P＜0.05);A组骨折移位、骨折分离及术后肺不张发生率低于B组(P＜0.05).结论 医源性断肋手术中应用环抱式肋骨接骨器一期修复内固定肋骨的患者术后创伤小,恢复快,疼痛轻.     

斑秃患者甲襞微循环改变及实验性治疗

目的；探讨斑秃病人甲襞微循环的改变及实验性治疗效果。方法：对门诊斑秃病人进行甲襞微循环观测，以正常人作对照。结果：32例斑秃病人甲襞微循环的清晰度稍有下降，异形管襻比正常人增多（P＜0．05）。红细胞有轻、中度聚集，血流速度减慢，部分病人有轻度襻周渗出，少数病人有襻顶出血。甲襞汗腺导管多于正常人，全秃病人又比一般斑秃病人多（P＜0．001）。654－2促进了毛发生长，且新生毛发不易脱落。结论：斑秃病人存在微循环障碍。654－2对治疗斑秃是有意义的。     

茄病镰刀菌性角膜炎2例

茄病镰刀菌性角膜炎２例漆军①虞瑞尧①＊赵华①冯毅②史云玲①谢灵③角膜真菌性感染的发病率逐渐上升，真菌性角膜炎能导致失明的严重后果。我们收治了２例茄病镰刀菌性角膜炎患者，报道如下。例１．女，３１岁，吉林四平市人，农民。患者于今年４月１７日劳动时，麦穗刺...     

卫生资源配置和利用的研究状况

卫生资源配置和利用是卫生系统政策与体制的基础，现对国内近年来卫生资源配置和利用方面的研究状况综述如下。     

山西省医学科技文献信息资源与服务平台内容评价

论述山西省医学科技文献信息资源自身特点：地方特色浓厚、价值较高，对此建设资源共享平台，建立山西省与全国同行业信息交流与传递的绿色通道，避免全省重复建设。具体从资源共享规模、共知程度、共用程度和获取便利性进行评价。     

洛沙坦和黄芪治疗糖尿病性肾病的相关机制研究

目的: 观察洛沙坦和黄芪对糖尿病大鼠肾脏病变的治疗作用并探讨其作用机制。方法:用SD大鼠复制链脲佐菌素糖尿病模型,12周后观察洛沙坦和黄芪对糖尿病大鼠肌酐清除率(Ccr)、尿白蛋白排泄率及肾小球平均体积、肾小球毛细血管基底膜平均厚度的影响,并观察血、肾脏局部一氧化氮(NO)、内皮素-1(ET-1)、转化生长因子-β₁(TGF-β₁)水平的变化。结果: 洛沙坦和黄芪可降低糖尿病大鼠Ccr、尿蛋白排泄率及肾小球平均体积、肾小球毛细血管基底膜平均厚度,并可不同程度降低肾脏局部NO、ET-1、TGF-β₁水平,两药合用可增强上述作用。 结论: 洛沙坦和黄芪可延缓糖尿病肾脏结构和功能损害的进程,且两药合用效果更佳,此作用可能与洛沙坦和黄芪降低肾脏局部NO、ET-1、TGF-β₁水平有关。     

节日礼品箱(盒)卫生管理措施

近几年来，每逢节假日，生产厂家、大型超市商店经营礼箱或礼品盒，以其外观大方、品种繁多、价格适中的特点，深受广大消费者喜爱，但这些礼品箱（盒）存有许多卫生问题有待解决。     

中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究

Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam.