转录因子Ets-1与肝纤维化关系的研究

转录因子Ets-1是Ets家族成员之一,与肝纤维化的关系日益受到人们的关注,它通过调节多种因子的基因转录,从而影响肝纤维化的发生和发展,本文将Ets-1在肝纤维化中的作用概述如下。     

蝎毒活性肽对福尔马林痛实验的影响

中医用全蝎或蝎尾治疗风湿痹痛、惊厥等症［１］。现代医学证明蝎毒具有抗肿瘤、抗炎、抗癫痫、镇痛等作用［２］。本实作者单位　４５３００３　河南新乡　新乡医学院医学系１９９６级（冯　晓，祝　琳，范书锋）；新乡医学院生理学教研室（张一红，丁延峰）验通过福尔马林痛模型，观察由小鼠腹腔注射蝎毒活性肽后的镇痛效应。１　材料与方法１．１　蝎毒活性肽　由新乡医学院肿瘤研究所提供，用ｐＨ６．８，浓度为１／１５ｍｏｌ·Ｌ－１的ＮａＨ２ＰＯ４－ＫＨ２ＰＯ４缓冲溶液稀释为浓度０．０１％的溶液。福尔马林溶液用福尔马林原液与…     

穿透性角膜移植治疗感染性角膜溃疡的临床观察

目的： 观察穿透性角膜移植术治疗感染性角膜溃疡的疗效。方法： 采用 Ｍ Ｋ 液保存的新鲜角膜材料对 ２６ 例 （２６ 只眼） 严重的细菌、真菌及单疱病毒性角膜溃疡行穿透角膜移植术, 术后随访 １２～１８ 个月。结果： 细菌、真菌及单疱病毒性角膜溃疡的术后植片透明率分别为 ７７７８％ 、５３８５％ 、２５％ , 术后主要的并发症有一过性高眼压、植片排斥及感染复发。结论： 对于药物治疗无效的严重的感染性角膜溃疡, 穿透性角膜移植可有效去除病灶, 重建眼球的完整性, 并为二期光学角膜移植奠定基础。     

Effect of nuclear factor-κB p65 ASOND on I typ collagen expression and rat hepatic stellate cells proliferation

Objective Sstudy effect of nuclear factor-κB ASOND on I type collagen expression and rat hepatic stellate cells(HSC)proliferation.Methods Rat HSCs were separated by affusing and digestingof Ⅳ type collagenenzyme and density acentric method.Lipid-mediated NF-κB p65 ASOND(0.001,0.01,0.1,1μmol/L)Was transferred into rat HSCs.Toxicity of HSCs caused by NF-κB p65 ASOND and activity of LDH were determined by trypan blue staining.Proliferation affection of transferring NF-κB p65 ASOND into HSCs was determined by MTT.In different concentration NF-κB p65 ASOND.expression of Ⅰ type collagen stimulated by 1mg/L TNF-αwas determined by RT-PCR and ELISA.Results After transfection of NF-κB p65 ASODN,expression of NF-κB protein in HSCs was decreasing.Toxicity experiment indicated that NF-κB p65 ASOND of different concentration(0.001,0.01,0.1 and 1.0 μmol/L)had no effect on HSCs livability and LDH activity(P<0.05).Four different concentration NF-κ B p65 ASOND could restrain HSCs proliferation stimulated by 1 mg/L TNF-α.The expression of I type collagen and mRNA stimulated by 1mg/LTNF-αwas increased,and had a positive correlation with concentration(P>0.05).Conclusion NF-κB p65 ASOND may depress NF-κB activity to restrain HSCs proliferation and Ⅰ type collagen expression,and reduce extracellular matrix.     

Effect of nuclear factor-κB p65 ASOND on I typ collagen expression and rat hepatic stellate cells proliferation

Objective Sstudy effect of nuclear factor-κB ASOND on I type collagen expression and rat hepatic stellate cells(HSC)proliferation.Methods Rat HSCs were separated by affusing and digestingof Ⅳ type collagenenzyme and density acentric method.Lipid-mediated NF-κB p65 ASOND(0.001,0.01,0.1,1μmol/L)Was transferred into rat HSCs.Toxicity of HSCs caused by NF-κB p65 ASOND and activity of LDH were determined by trypan blue staining.Proliferation affection of transferring NF-κB p65 ASOND into HSCs was determined by MTT.In different concentration NF-κB p65 ASOND.expression of Ⅰ type collagen stimulated by 1mg/L TNF-αwas determined by RT-PCR and ELISA.Results After transfection of NF-κB p65 ASODN,expression of NF-κB protein in HSCs was decreasing.Toxicity experiment indicated that NF-κB p65 ASOND of different concentration(0.001,0.01,0.1 and 1.0 μmol/L)had no effect on HSCs livability and LDH activity(P<0.05).Four different concentration NF-κ B p65 ASOND could restrain HSCs proliferation stimulated by 1 mg/L TNF-α.The expression of I type collagen and mRNA stimulated by 1mg/LTNF-αwas increased,and had a positive correlation with concentration(P>0.05).Conclusion NF-κB p65 ASOND may depress NF-κB activity to restrain HSCs proliferation and Ⅰ type collagen expression,and reduce extracellular matrix.     

新型农村合作医疗基金安全管理的财务思考

新型农村合作医疗已在部分地区开始试点,从实际调查的情况分析,基金安全管理方面,制度尚欠规范。本文从财务的视角,探索这一新型农村合作医疗基金的安全建设。     

上海市老年人桡、尺骨骨矿含量分析和绝经对妇女骨矿含量的影响

应用单光子吸收技术调查分析了60岁以上男性578名、女性557名桡、尺骨骨矿含量与绝经对妇女骨矿含量的影响。结果表明60岁以上的骨矿含量较30～89岁明显降低,女性年丢失率为男性的2～8倍,随增龄女性骨矿低于男性的程度越益明显,绝经年限对骨矿含量有阴显影响。老年人骨矿丢失的特点为老年保健提出了新的课题。     

一例过量医疗照射诱发肉瘤

郭某，女，９岁。辽宁锦州人。左大腿放射性溃疡伴肉瘤。１９８９年２月某日午夜因发热在当地卫生院作肌肉注射时，误将针头断在臀部肌肉内，在Ｘ射线透视下手术取针约３～４小时，致局部组织过量受照。一年后（１９９０年）受照部位逐渐形成硬块、隆起。１９９５年１１月...     

48例川崎病报告

现就本院１９８８～２０００年间收治４８例川崎病作以下分析。１临床资料１ １一般资料 :４８例中男３２例 ,女１６例。发病年龄４月～７岁 ,４岁以下３２例 ,占７０ % ,＜１岁１０例。无明显季节变化。１ ２临床表现 :所有病例均有发热 ,入院时发热持续＞５天２８例 ,其中＞７天２０例 ,最长１５天 ;热型不规则 ,平均热程１１ ３天。４１例出现多形皮疹 ,出疹时间不定 ,一般部位较局限 ,以躯干部为多 ,但均无疱疹及结痂。颈淋巴结肿大者３４例 ,大小不等 ,在０ ５～３ ５ｃｍ之间 ,轻压痛 ,非化脓性。两眼结膜充血者３０例 ,杨梅舌２９例…     

实验性角膜新生血管的形态观察

目的观察兔角膜新生血管的形态特点,探讨其发生机制及治疗效果.方法将16只新西兰兔随机分为实验和对照两组,均以75%硝酸银液烧灼兔角膜,实验组于硝酸银烧灼后行β射线照射,观察两组角膜新生血管的生长规律;选期制做新生血管铸型,扫描电镜下比较两组间新生血管形态差异.结果角膜新生血管以典型的芽生方式发生和增殖,经β射线照射后的血管芽形成受到明显的抑制和破坏,新生的血管支干发生萎缩、坏死.结论阻断新生血管的芽生过程可抑制角膜新生血管的形成和发展.