角膜塑形术患儿用眼健康行为评定问卷的构建与验证

目的 研制角膜塑形术患儿用眼健康行为测评工具,为针对性干预提供参考.方法 采用文献分析、访谈法构建问卷的初始条目池,选取20名专家进行2轮函询形成初始问卷,选取714例角膜塑形术患儿进行调查以进行信度和效度检验.结果 形成用眼时间、用眼环境、用眼行为、饮食和运动、戴镜行为5个维度共48个条目的角膜塑形术用眼健康行为评定...     

Expression and significance of β-catenin and peroxisome proliferator-activated receptor-γ in hepatocellular carcinoma

Objective To investigate the expression and significance of β-catenin and peroxisome prolifera-tot-activated receptor-γ,(PPARγ) in bepatocellular carcinoma. Methods Tissue microarrays were established to detect β-catenin and PPARγ expression in 49 cases of hepatocellular carcinoma,49 cases of adjacent nontumoral liv-er tissue and 6 cases of normal liver tissue. The relationships between PPARγ and β-catenin as well as between PPARγ and clinicopathological parameters were observed. Results The aberrant expression rate of β-catenin was 69.39%,48.98 % and 0 respectively (P=0.001). The positive expression rate of PPARγ was 51.02%,30.61% and 0 respectively (P=0.016). Clinicopathological analysis revealed that the increase of PPARγ expression was not associated with age,tumor size,serum alpha fetoprotein (AFP) levels,tumor embolus of portal vein or inferior vena cava,and HBsAg infection(χ2=0.214,3.201,0.046,3.201,P>0.05 for each),but correlated with differentiation grades(χ2=4.693,P<0.05). Aberrant expression of β-catenin was associated with PPARγ expression(χ2= 5.130,P<0.05). Conclusion Aberrant expression of β-catenin may involve in the liver carcinogenesis. The high expression of PPARγ in hepatocellular carcinoma is significantly correlated with the clinicopathological characteris-tics. Detection of PPARγ is valuable for diagnosing hepatocellular carcinoma,and evaluating malignancy extent and prognosis.     

Expression and significance of β-catenin and peroxisome proliferator-activated receptor-γ in hepatocellular carcinoma

Objective To investigate the expression and significance of β-catenin and peroxisome prolifera-tot-activated receptor-γ,(PPARγ) in bepatocellular carcinoma. Methods Tissue microarrays were established to detect β-catenin and PPARγ expression in 49 cases of hepatocellular carcinoma,49 cases of adjacent nontumoral liv-er tissue and 6 cases of normal liver tissue. The relationships between PPARγ and β-catenin as well as between PPARγ and clinicopathological parameters were observed. Results The aberrant expression rate of β-catenin was 69.39%,48.98 % and 0 respectively (P=0.001). The positive expression rate of PPARγ was 51.02%,30.61% and 0 respectively (P=0.016). Clinicopathological analysis revealed that the increase of PPARγ expression was not associated with age,tumor size,serum alpha fetoprotein (AFP) levels,tumor embolus of portal vein or inferior vena cava,and HBsAg infection(χ2=0.214,3.201,0.046,3.201,P>0.05 for each),but correlated with differentiation grades(χ2=4.693,P<0.05). Aberrant expression of β-catenin was associated with PPARγ expression(χ2= 5.130,P<0.05). Conclusion Aberrant expression of β-catenin may involve in the liver carcinogenesis. The high expression of PPARγ in hepatocellular carcinoma is significantly correlated with the clinicopathological characteris-tics. Detection of PPARγ is valuable for diagnosing hepatocellular carcinoma,and evaluating malignancy extent and prognosis.     

某部队医院住院患者心理健康状况调查

目的：了解某部队医院住院患者心理健康状况及影响因素。方法：对入住该院的6个科室患者进行症状自评量表、社会支持量表及医院焦虑抑郁量表的测评并分析结果。结果：军人与非军人的SCL-90各项因子得分差异除强迫因子外均有统计学意义,手术患者与非手术患者、病重患者与普通患者的躯体化因子得分差异有统计学意义;HAD中军人与非军人的抑郁、焦虑得分差异均有统计学意义,手术与非手术患者的抑郁因子得分差异有统计学意义;相关分析结果显示SCL-90得分与社会支持总分呈负相关,焦虑、抑郁得分与社会支持总分呈负相关。结论：军人的心理健康状况高于一般人群,手术或病重可能会影响患者的心理健康状况,社会支持因素及文化程度对患者心理状况有一定影响。     

苦杏仁中杏仁苷的研究进展

苦杏仁苷是苦杏仁的主要有效成分,它已成为医药上常用的祛痰止咳剂、辅助性抗癌药物,现代研究表明,苦杏仁苷具有抗肿瘤、抗溃疡、抗脑缺血、调节免疫功能等药理作用。因此,开展苦杏仁苷的结构、药理研究和利用对现代医学有重要意义。     

MCM5和Ki-67在乳腺癌组织中的表及意义

目的:探讨微小染色体维持蛋白5(MCM5)和Ki-67基因蛋白在乳腺癌组织中的表达及意义.方法:用免疫组织化学SP方法对手术诊断的142例乳腺癌组织与40例正常乳腺组织中MCM5和Ki-67基因蛋白进行检测,分析其表达情况.结果:MCM5、Ki-67在乳腺癌与正常组织中皆有表达,乳腺癌组织的表达明显高于正常组(P＜0.05).MCM5表达和Ki-67表达呈正相关(相关系数r=0.486P=0.031).MCM5表达与乳腺癌组织分级、临床分期、淋巴结转移有关(P＜0.05).Ki-67与临床分期、淋巴结转移有关(P＜0.05).结论:MCM5和Ki-67的表达与乳腺癌的发生、发展有关,可能成为新的乳腺癌肿瘤增生标志物.     

VEGF、ICAM-1和ADA在良恶性胸腔积液中的鉴别诊断

目的:研究血管内皮生长因子(VEGF)、细胞问黏附分子1(ICAM-1)和ADA在良恶性胸腔积液形成过程中的作用.方法:81例胸腔积液患者分成4组:27例胸液细胞学检查阳性的肺癌患者、16例胸液细胞学检查阴性的肺癌患者、26例结核性胸腔积液,12例其它病因(8例低蛋白血症、4例心衰)患者.采用双抗体夹心法(EL ISA)分别检测胸液中可溶性血管内皮生长因子(sVEGF)、可溶性细胞间黏附分子1(sICAM-1)和ADA的表达水平.结果:胸液中sVEGF、sICAM-1和ADA的含量为,肺癌恶性胸液细胞学检查阳性组[(1017±730)pg/ml,(1510±597)ng/ml,(149±102)ng/ml]、肺癌恶性胸液细胞学检查阴性组[(610±520)pg/ml,(951±390)ng/ml,(118±82)ng/ml]、结核性胸液组[(449±522)pg/ml,(1037±371)ng/ml,(179±132)ng/ml]和其它病因组[(126±78)pg/ml,(88±43)ng/ml,(30±12)ng/ml].各组之间的sVEGF相比均差异有显著性(P<0.05),而恶性与结核性胸液中的sICAM-1和ADA均明显高于其它病因组(P<0.01).胸液中的sVEGF和sICAM-1与乳酸脱氢酶(LDH)水平呈显著性正相关(r=0.58,0.38;P<0.05),ADA与腺苷脱苷酶(ADA)呈正相关(r=0.38,P<0.05).结论:恶性胸腔积液的形成与VEGF密切相关,同时ICAM-1和ADA也在恶性胸液的形成机制中起着重要的作用,而ADA可能与结核性胸膜炎的纤维化形成的组织重建有关.     

用离子色谱法测有害作业场所中酸雾的改动

目的改动的离子色谱法主要参数是:淋洗液KOH,浓度15m M,进样量10ul;混合标液的配置浓度改成,CL-1(10mg/l)、SO4-2(10mg/l)。变动后的方法其线性相关系数以及精确度和准确度符合要求,且更能满足实际工作的需要。