制备小鼠去细胞拟胚体对Lewis肺癌细胞的促分化作用

BACKGROUND: Co-culture with embryonic stem cells or embryonic tissues can induce differentiation of carcinoma cells into normal epithelial cells or decrease malignancy of carcinoma cells. Acellular embryoid bodies retain the structure and important cytokines of embryonic tissues. OBJECTIVE:To prepare acellular embryoid bodies from mouse embryonic stem cells and to investigate their effects on differentiation of mouse Lewis lung carcinoma cells at three-dimensional culture in vitro. METHODS: Mouse embryonic stem cells (D3) were dynamically cultured for 7 days to produce embryoid bodies followed by decellularization with 0.1% sodium dodecyl sulfate. Mouse Lewis lung carcinoma cells were co-cultured with acellular embryoid bodies as test group or cultured in three-dimensional matrigel medium for 7 days as control group, respectively. Cell proliferation and expression of E-cadherin were detected by immunohistochemical staining and western blot assay, respectively. In addition, mRNA expressions of Slug and E-cadherin were observed using RT-PCR technology. RESULTS AND CONCLUSION: Uniform mouse embryoid bodies were successfully prepared, and were completely decellularized with sodium dodecyl sulfate. After 7-day three-dimensional matrigel culture, in the control group, multicellular tumor spheroids were formed, accompanied by a higher Ki67 positive rate; Lewis lung carcinoma cells in the test group were repopulated in the acellular embryoid bodies showing significantly lower Ki67 positive rate. Compared with the control group, the absorbance of Paxillin in the test group was significantly smaller, and the absorbance of E-cadherin was significantly higher (P < 0.05). Besides, mRNA expressions of Slug and E-cadherin were significantly decreased and increased in the test group compared with the control group, respectively (P < 0.05). These findings indicate that the acellular embryoid bodies can promote differentiation of mouse Lewis lung carcinoma cells in three-dimensional culture in vitro. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松; ORCID: 组织工程0000-0003-2685-4218(吕卫东)     

肝癌纵隔及锁骨上淋巴结转移1例报告并文献复习

原发性肝癌(primary carcinoma of liver, PLC)是肝细胞和(或)肝内胆管上皮细胞发生的恶性肿瘤,是我国常见、高发的恶性肿瘤之一,在西方发达国家也在升高[1].因受肝脏解剖和生理因素影响,其发生肝内肝外转移较早,侵犯部位较多,转移机制复杂,一经发现约80%的患者便失去手术机会,且临床治疗效果及预后差,受到世界广泛关注.大多数肝癌以血行转移为主(如肺、骨、肾上腺等),伴有淋巴结转移的肝癌并不多见,且多是首先出现在腹腔淋巴结(肝门、腹主动脉旁淋巴结),而出现纵隔、锁骨上淋巴结转移临床少见.笔者经治1例肝癌纵隔、锁骨上淋巴结转移患者,结合文献资料报道如下.     

热消融治疗肺部亚实性结节专家共识(2021年版)

局部热消融技术在肺部结节治疗领域正处在起步与发展阶段,为了肺结节热消融治疗的临床实践和规范发展,由"中国医师协会肿瘤消融治疗技术专家组""中国医师协会介入医师分会肿瘤消融专业委员会""中国抗癌协会肿瘤消融治疗专业委员会""中国临床肿瘤学会消融专家委员会"组织多学科国内有关专家,讨论制定了"热消融治疗肺部亚实性结节专家共...     

国产吻合器附加食管斜形切除新方法临床应用(附143例食管癌弓上吻合报告)

目的　探讨国产GF—Ⅰ型吻合器附加食管斜形切除新方法预防吻合口并发症的临床效果。方法　 1992年 4月至 2 0 0 3年 3月连续对食管中段癌采用弓上机械吻合 ,在切除肿瘤上端食管时 ,与食管长轴呈 3 5°～ 45°斜形角度 ,常规胃底组织包埋加固吻合口。结果　术后 3周钡剂造影 ,吻合口均通畅 ,呈规则或不规则椭圆形。无吻合口瘘 ,仅 1例术后 4个月发生狭窄 ,经扩张置管后治愈。胃液返流情况 :明显返流占 16 2 % ;轻度返流占 45 9% ;无返流占 3 7 8%。结论　此方法能明显改进机械吻合效果 ,显著降低吻合口瘘、狭窄及胃液返流等并发症 ,具有临床实用价值。     

新辅助化疗对非小细胞肺癌患者淋巴细胞免疫功能的影响及治疗

OBJECTIVETo observe the effects of neoadjuwant chemotherapy and neoadjuwant chemotherapy plus KLT on cell immunofunction in patients of NSCLC and therapy.METHOD26 patients of NSCLC were rolled in A team,treated with neoadjuwant chemotherapy only;and 28     

乳腺放线菌病诊断与治疗(病例报告与文献复习)

乳腺放线菌病非常罕见,教科书多不提及,临床易误诊为肿瘤而常常误治。作者收治一原发性乳线放线菌病,结合文献资料予以报告。1病案报告患者女性,20岁,已婚农民,以右乳肿块伴疼痛6月,长大1月之主诉于1997年1月8日入院,住院号970O25。6个月前发现右乳外侧有一花生米大小之肿块伴轻微疼痛,1月前肿块长大至3．5cm大小,继之出现局部红肿疼痛,当地医院按炎症治疗1周,疼痛明显缓解而肿块继续长大,以此收住本院。查体：一般状况良好,体温36SC,锁骨上淋巴结不大,心肺无异常。右乳明显隆起,外侧肿块10．OX7．．ocm,质硬、边界欠清…     

亚甲蓝在乳腺癌前哨淋巴结活检中的应用

目的探讨亚甲蓝作为蓝染料在乳腺癌前哨淋巴结活检中的应用价值。方法对已确诊的72例乳腺癌病人亚甲蓝皮下注射后行腋窝前哨淋巴结切除,送冰冻病理检查,再行乳腺癌改良根治术,并常规腋窝淋巴结清扫,术后病理石腊切片检查前哨淋巴结及手术切除标本。结果本组病例中68例共检出前哨淋巴结132枚,失败4例。前哨淋巴结的检出率94.4%,准确率91.8%,敏感度92%,假阴性率8%,假阳性率为0。结论采用亚甲蓝作为蓝染料应用于乳腺癌前哨淋巴结活检术,其前哨淋巴结活检的各项指标与其他蓝染料(专利蓝)相当,且材料广泛、费用低廉,具有临床应用前景。     

Vitexicarpin诱导肺癌A549细胞凋亡和对细胞周期作用的研究

目的:研究蔓荆子黄素(Vitexicarpin)对人肺癌细胞株A549细胞增殖和凋亡的影响及其机制.方法:设计长春新碱(Vineristine)阳性对照组和空白阴性对照组及不同浓度的Vitexiearpin溶液作用于A549细胞,应用细胞增殖抑制试验和流式细胞仪检测技术,观察Vitexicarpin对A549细胞增殖的影响,以及其诱导A549细胞在不同时段的凋亡情况和对细胞周期的影响.结果:Vitexicarpin浓度为10μmol/L时对A549细胞的增殖抑制率超过50%,IC50为8.21μmol/L.另外,Vitexicarpin能有效诱导A549细胞凋亡.从Vitexicarpin作用4h开始,细胞被阻滞于G2/M期.随时间增加,G1/S期细胞逐渐减少,G2/M期细胞逐渐增多,且凋亡细胞逐渐增多.结论:Vitexicarpin能抑制A549细胞增殖,诱导A549细胞发生凋亡,将细胞阻滞于G2/M期是其可能作用机制.     

食管斜形切除粘膜外翻斜形插入新术式的临床研究(附176例临床报告)

目的：为了预防食管胃吻合口并发症,作者自１９８７年９月－１９９７年４月设计一种主式应用于临床研究。方法：在肿瘤上方斜形切除食管,粘膜层１￣１．５ｃｍ长,且外翻覆盖在肌层上,然后将食管端２￣２．５ｃｍ插入胃腔内,用胃切吻合口人一层与食管肌层缝合一周,并在其上方再包埋缝合一层。结果：采用此方法连续治疗食管癌、贲门癌１７６例,术后未发生任何吻合口并发症。结论：未术式在许多关键步骤上有别于传统吻合方法,对     

Stathmin 基因在非小细胞肺癌中的表达

目的：检测 Stathmin 基因在非小细胞肺癌组织中的表达,探讨其与临床病理因素的关系。方法：用免疫组织化学 SP 法检测135例非小细胞肺癌及20例癌旁肺组织中 Stathmin 蛋白的表达,分析其与患者年龄、性别、肿瘤分化程度等临床病理特征之间的相关性。结果：小细胞肺癌组织中 Stathmin 基因蛋白的表达水平明显高于癌旁肺组织（P ＜0．05）,Stathmin 的表达与淋巴结转移有关（P ＜0．05）。结论：Stathmin 基因在非小细胞肺癌组织中的表达显著上调;Stathmin 基因的表达与淋巴结转移有关,而与年龄、性别、肿瘤大小、TNM分期、组织类型无关。