雷公藤多甙对大鼠附睾尾精子作用的扫描电镜观察

SD大鼠17只,分三组。实验组(二组)每天分别胃针灌服悬混于羧甲基纤维素钠(CMC)的雷公藤多甙,20mg/kg(6鼠)和30mg/kg(5鼠),每周6天,共4周,对照组(6鼠)灌服等量CMC。各组分别于第2和第4周各剖杀2-3只动物,主要做扫描电镜观察。结果说明GTW对附睾精子的作用可能先于睾丸,但随剂量的增大及作用时间的延长而逐渐波及曲细精管。对GTW最敏感的结构可能主要是鞭毛膜等膜性结构,对精于膜的损伤似乎先波及尾部,再损及头颈段。     

昆明山海棠抗生育作用的形态学研究：Ⅰ.睾丸,附睾的组织化学观察

本文应用组织化学方法探讨昆明山海棠(TH)对雄性大鼠睾丸,附睾的DNA、RNA.乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、ATP酶,葡萄糖-6-磷酸脱氢酶(G-6PDH)等的影响。成年雄性SD大鼠用昆明山海棠根50%乙醇提取物灌胃给药,剂量1.0g/kg/日,每周六次,共6周,所有用药鼠均丧失生育力。用药鼠附睾管腔内精子减少或完全消失,并可见少量脱落和退化的生精细胞(主要是精子细胞)。附睾头、尾部管腔内精子大部分呈断头、卷尾等畸形。精子LDH.SDH活性明显下降。精子细胞及残余体RNA凝集成块,而精原细胞、精母细胞,支持和间质细胞及附睾各段上皮细胞的形态和组织化学均未见明显改变。本实验表明,TH主要影响精子细胞RNA的功能及精子糖代谢,而对其它生精细胞无明显作用。     

Effects of Seminal Plasma Relaxin on Human Sperm Motility

To clarify the role of endogenous relaxin on sperm motility, relaxin in semen was neutraliged by anti-relaxin antibody in vitro.22 semen samples were collected from infertility clinic and tested with Hamilton-Thorn 2000 Motility Analyzer to detect spermmotility(%),progressive motility(%),path velocity (micro/sec) and velocity(0-4 grade) at the time of 0,15,30 and 60 min respectively.The results showed that sperm motility declined significantly after being incubated with anti-relaxin serum.Sperm progressive motility declined more obviously.This experiment revealed that endogenous relaxin could play an important role in the physiological process of maintaining sperm motility,especially progressive motility.     

Effects of Seminal Plasm a Relaxin on Hum an SpermMotilit

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