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101.
Rong-Hua Tao Li Bai Zuzana Berkova Jillian Wise Rezaeian Hossein Urszula Daniluk Xue Ao Felipe Samaniego 《Clinical Lymphoma, Myeloma & Leukemia》2011
Presence of potential inhibitors of Fas could block Fas signaling and explain cancer cells resistance to apoptosis. In this study, we found that PMLRARα binds to Fas and blocks Fas-mediated apoptosis through recruiting c-FLIP in APL. Targeting tissue-specific inhibitors of Fas such as PMLRARα would improve cancer therapy. 相似文献
Full Abstract
Fas plays a critical role in cell proliferation and in the selective killing of autoreactive lymphocytes and abnormal cells, including infected cells. To explain the common expression of Fas and the resistance to the Fas-induced apoptosis observed in some normal and cancer cells, we have screened cells for potential regulators of the Fas death receptor. By using mass spectrometry analysis of Fas-associated proteins, we identified peptides derived from promyelocytic leukemia (PML).PML enhances102.
103.
Both endogenously produced and exogenously administered H2S exert numerous biological effects. However, the molecular mechanisms underlying these effects are not fully understood. This review surveys the biological effects of H2S and summarizes the molecular mechanisms of H2S action. It focuses on the role of H2S/HS--induced NO release from nitroso compounds, modulation of ion channels and the antioxidant and radical properties of H2S in the molecular mechanism of its effects. The potential involvement of H2S in nitroso signaling underlying its diverse biological effects is also discussed. 相似文献
104.
105.
Zuzana Skodova PhD Iveta Nagyova Jitse P. van Dijk Adriana Sudzinova Helena Vargova Jaroslav Rosenberger Berrie Middel Martin Studencan Sijmen A. Reijneveld 《International journal of public health》2009,54(4):233-240
Objectives: The aim of this article is to explore socioeconomic inequalities in the psychological characteristics (psychological well-being,
perceived mental health status) and perceived quality of life among cardiac patients.
Methods: A structured interview was conducted with 362 patients (32% women, mean age 56 ± 7.3 years) referred for coronary angiography.
The GHQ-28 was used to measure psychological well-being, the SF-36 for perceived mental health status. Income and education
indicated socioeconomic position. Logistic regressions were employed, adjusted for age, gender, functional status and severity
of disease.
Results: Patients with low income or education had a higher probability of having poor psychological well-being compared to participants
with high income or education (OR 5.5,CI 2.32-12.80; OR 3.1,CI 1.52-6.37 resp.), and were also more likely to have worse mental
health status (OR2.9,CI 1.02-8.51;OR 4.8,CI 1.36-16.99 resp.), and low quality of life (OR 2.9,CI 1.02-8.51; OR 4.8,CI 1.36-16.99
resp.).
Conclusions: Socioeconomic status was found to be negatively associated with the psychological outcomes and quality of life among cardiac
patients. Socioeconomic inequalities should be taken into account when designing suitably-adapted interventions focusing on
psychosocial factors among cardiac patients.
Submitted: 27 September 2007; revised: 08 January 2009, 16 March 2009; accepted: 18 March 2009 相似文献
106.
Navrátil Tomá? Hojdová Maria Rohovec Jan Pení?ek Vít Va?ilová Zuzana 《Bulletin of environmental contamination and toxicology》2009,83(2):269-274
In year 2006, 17.9 ha of forest was burned during a forest fire at the Bohemian Switzerland National Park found in northern
part of Czech Republic (CR), central Europe. Complete combustion of organic soil (4,039 t) on the burned area caused volatilization
of 1.34 ± 0.07 kg of Hg. Thus Hg emissions due to fire amounted to 75.1 g ha−1. The average burned forested areas in CR for the period 2000–2006 were reported at 356 ha with estimated Hg emissions at
26.7 kg year−1, while the average anthropogenic emissions in the same period amounted to 3 t year−1. Thus estimated mean emissions of Hg from burned forest soil in the period 2000–2006 reached 1% of the annual anthropogenic
Hg emissions. 相似文献
107.
Brillowska-Dabrowska A Wianecka M Dabrowski S Mladenovska Z Kur J Ahring BK 《Scandinavian journal of clinical and laboratory investigation》2008,68(8):720-730
A DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide. A long oligonucleotide containing the primer site and the specific 9 nt 3 prime end, which is complementary to specific 9 nt, cohesive 3 prime end of the TspRI genomic DNA fragment, and a short, degenerated, oligonucleotide covering the remaining TspRI cohesive ends. Other cohesive ends are covered by a short degenerated oligonucleotide lacking the primer site. The ligation mixture is used as a template for amplification using a single primer corresponding to the 5 prime end of the long, specific oligonucleotide. The selection of TspRI digested genomic DNA fragments for amplification is achieved by sequence selective ligation of the specific long oligonucleotide carrying the primer site to both ends of the specific target fragment. This technique allows for differentiation of the organisms without previous knowledge of their DNA sequence. The usefulness of the method is confirmed by genotyping of 70 previously characterized clinical E. coli isolates. The grouping obtained was identical to the results of REA-PFGE. Versatility of the method is highlighted, i.e. its combining the advantages of the AFLP technique with a simple, rapid and cheap polymerase chain reaction product detection method. 相似文献
108.
Tatiana Dubayova Iveta Nagyova Eva Havlikova Jaroslav Rosenberger Zuzana Gdovinova Berrie Middel Jitse P. van Dijk Johan W. Groothoff 《Quality of life research》2009,18(1):33-42
Purpose Personality traits appear as determinants of quality of life (QoL) in most chronic diseases. The aim of this study is to explore
whether neuroticism and extraversion contribute to the variance in QoL in patients with Parkinson’s disease (PD) when controlled
for age, functional status and disease duration.
Methods The Parkinson’s Disease Quality of Life Questionnaire (PDQ-39) was used to assess QoL and the Unified Parkinson’s Disease
Rating Scale (UPDRS) for disease severity. Neuroticism and extraversion were measured with the Eysenck Personality Questionnaire
(EPQR-A). Multiple linear regression analysis was then used to assess the contribution of neuroticism and extraversion to
QoL.
Results The sample consisted of 153 PD patients (48.4% women; 67.9 ± 9.3 years; mean disease duration 7.5 ± 5.8 years). Neuroticism
was, after disease severity, the second most important variable associated with QoL in PD patients, in particular for domains
associated with psychological processes: emotional well-being, social support, stigma and communication. A higher score in extraversion was significantly associated with better emotional well-being in males, but surprisingly, with worse emotional well-being in females.
Conclusions After functional status, personality traits were clearly associated with QoL in PD patients. Therefore, they should be taken
into account by health-care professionals in their appraisal of patient complaints. 相似文献
109.
Song H Vita M Sallam H Tehranchi R Nilsson C Sidén A Hassan Z 《Cancer chemotherapy and pharmacology》2007,60(6):841-849
Myelosuppression is one the most frequent side effects of chemotherapy. New agents that more selectively target cancer cells
have been developed in attempt to improve the effects and to decrease the side effects of cancer treatment. Roscovitine is
a purine analogue and cyclin-dependent kinase inhibitor. Several studies have shown its cytotoxic effect in cancer cell lines
in vitro and in xenograft models in vivo. In this study, we investigated the effect of roscovitine on hematopoietic progenitors
in vitro and in vivo in mice. The clonogenic capacity of hematopoietic progenitors was studied using burst-forming unit-erythroid
(BFU-E), colony-forming unit granulocyte, macrophage (CFU-GM) and colony-forming unit granulocyte, erythroid, macrophage,
megakaryocyte (CFU-GEMM). In vitro, bone marrow cells were exposed to roscovitine (25–250 μM) in Iscove’s modified Dulbecco’s
media for 4 h or to roscovitine (1–100 μM) in MethoCult media for 12 days. No effect on colony formation was observed after
exposure to roscovitine for 4 h; however, concentration- and cell type-dependent effects were observed after 12 days. Roscovitine
in concentration of 100 μM inhibited the growth of all types of colonies, while lower concentrations have shown differential
effect on hematopoietic progenitors. The most sensitive were CFU-GEMM, followed by BFU-E and then CFU-GM. In vivo, mice were
treated with single dose of roscovitine (50, 100 or 250 mg/kg) and the effect on bone marrow was studied on day 1, 3, 6, 9
or 12 after the treatment. In the second part of experiment, the mice were treated with roscovitine 350 mg/kg/day divided
into two daily doses for 4 days. The bone marrow was examined on day 1 and 5 after the last dose of roscovitine. On day 1,
BFU-E decreased to less than 50% of the controls (P = 0.019). No decrease in BFU-E formation was observed on day 5. No significant effect was observed on CFU-GM and CFU-GEMM
growth after the treatment with multiple doses of roscovitine. Single doses of roscovitine or dimethylsulfoxide did not affect
the colony formation. We also studied the distribution of roscovitine to the bone marrow after a dose of 50 mg/kg was administered
intraperitoneally. Only 1.5% of the drug was detected in the bone marrow. Thus, the roscovitine effect on hematopoietic progenitors
in bone marrow in vivo is only transient. One reason may be that only a small fraction of roscovitine reaches the bone marrow.
Another explanation may be the short half-life observed for roscovitine that might not allow enough cell exposure to the drug.
However, the toxicity of roscovitine to hematopoietic progenitors in vitro is within the same exposure range as cytotoxicity
to cancer cells. Thus, precaution should be taken in clinical trials, especially when combinations with myelosuppressive cytostatics
are used.
Hairong Song and Marina Vita contributed equally to this work. 相似文献
110.
Siniscalchi A Piraccini E Miklosova Z Bagni A D'Errico A Cucchetti A Lauro A Pinna AD Faenza S 《Transplantation》2007,84(3):346-350
BACKGROUND: Analysis of intraoperative changes of metabolic, hemodynamic, and coagulative parameters is useful to detect early ischemia-reperfusion damage after intestinal transplant. METHODS: The objective of our study is to correlate the histological damage at the end of transplant in relation to the intraoperative changes after reperfusion. The histological aspect was graded according to Park's classification at the end of the surgical procedure with biopsies of the graft. Patients were divided into two groups according to the presence or absence of histological damage of the small bowel wall: group A (normal mucosa/minimal damage: Park's grades 0-1) and group B (mucosal damage: Park's grades 2-8). RESULTS: Significant hemodynamic, metabolic, and coagulative disorders were observed in group B. Consequently, these disorders are thought to be early indicators of graft damage. CONCLUSIONS: Actual monitoring procedures used for postoperative graft surveillance remain paramount in detecting postoperative intestinal dysfunction, but the indicators described in this paper could represent a further help in intraoperative and postoperative management. 相似文献