Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats

The mitochondrial calcium uniporter (MCU) transports Ca²⁺ from the cytoplasm to the mitochondrial matrix and thus maintains Ca²⁺ homeostasis. Previous studies have reported that inhibition of MCU by ruthenium red (RR) protects the brain from ischemia/reperfusion (I/R) injury and that mitochondrial fission plays an important role in I/R injury. However, it is still not known whether MCU affects mitochondrial fission. In the present study, treatment with RR was found to decrease the concentration of free calcium in the mitochondria, calcineurin enzyme activity and dynamin-related protein 1 expression, and treatment with spermine was found to have the opposite effect in organisms subjected to occlusion of the middle cerebral artery lasting 2 h followed by 24 h reperfusion. These results indicate that MCU may be related to mitochondrial fission via modulating mitochondrial Ca²⁺ uptake and this relationship between MCU and mitochondrial fission may protect the brain from I/R injury.     

胎羊单侧输尿管梗阻后肾脏病理相关研究

【摘要】〓目的〓探讨胎儿期发生单侧输尿管梗阻后,梗阻侧肾脏的病理变化过程。方法〓对孕75~85天的胎羊实行手术造成其单侧输尿管不完全性梗阻,在术后不同时期,取双侧肾脏(对侧肾脏作为对照),进行大体标本、组织学和分子学(PAX2和VEGF的表达)的检测。结果〓梗阻侧肾脏,表现为皮质变薄,皮质囊性改变、间质纤维化、肾小球数目减少;PAX2表达显著升高,而VEGF表达明显减少。结论〓在胎羊模型中,输尿管发生梗阻后,梗阻侧肾脏随之而来发生明显的病理变化。     

结核感染T细胞斑点试验在脊柱结核诊断中的应用

目的：探讨结核感染T细胞斑点试验在脊柱结核诊断中的临床应用价值。方法选取2012年4月～2013年1月本院骨科病区收治的疑似脊柱结核患者156例,分别采用结核感染T细胞斑点试验和结核菌素试验进行诊断,比较两者对脊柱结核诊断的临床意义（包括灵敏性、特异性、假阳性率和假阴性率指标的差别）。结果结核感染T细胞斑点试验的灵敏性和特异性明显高于结核菌素试验（P＜0．05）;同时,结核感染T细胞斑点试验的假阳性率和假阴性率明显低于结核菌素试验试验（P＜0．05）。结论结核感染T细胞斑点试验在诊断脊柱结核中具有良好的敏感性和特异性,具有重要临床应用价值。     

两性霉素B常见不良反应的临床观察与护理

两性霉素B属大环内酯多烯类,用于治疗深部真菌感染,由于治疗时间长,不良反应多。因此,总结我科8例应用该药发生不良反应的临床观察与护理,以期减少其不良反应对治疗的影响,提高药物的临床使用效果。     

Role of IL‐17 and TGF‐β in peritoneal adhesion formation after surgical trauma

Peritoneal adhesions are fibrous tissues formed after surgery. Both cytokines and transforming growth factors (TGFs) are involved in this process. The objective of this study was to investigate the cross talk between these entities. Peritoneal drainage fluid after surgery from patients and rodent models was examined by enzyme‐linked immunosorbent assay and fluorescence‐activated cell sorter. Data showed that the concentrations of interferon (IFN)‐γ and interleukin (IL)‐17 reached their peaks 6–12 hours after surgery, whereas TGF‐β1 concentrations showed two postoperative peak time points at 2 and 72–96 hours. By neutralizing IFN‐γ, IL‐17 6–12 hours, and TGF‐β1 72–96 hours after surgery, the degree of adhesion reduced significantly. However, neutralizing TGF‐β1 2 hours after surgery did not affect adhesion formation. Furthermore, in vitro studies showed that compared with the fibroblasts that were directly stimulated with TGF‐β1, the prestimulation of IL‐17 promoted plasminogen activator inhibitor‐1 production while inhibiting tissue‐type plasminogen activator production. Moreover, additional stimulation with IFN‐γ enhanced this effect. Together, these data indicate that IL‐17 may promote adhesion formation by increasing the reaction of fibroblasts against TGF‐β1. Blocking IL‐17 might have a therapeutic potential in preventing adhesion formation after surgery.     

132℃化学指示卡变色不均消除法

１３２℃化学指示卡变色不均消除法ＥｌｉｍｉｎａｔｉｏｎｏｆＣｏｌｏｒ－ｃｈａｎｇｉｎｇＮｏｎｕｎｉｆｏｒｍｏｆｔｈｅ１３２℃ＣｈｅｍｉｃａｌＩｎｄｉｃａｔｉｎｇＣａｒｄ果海青梁安珍ＧｕｏＨａｉｑｉｎｇ，ＬｉａｎｇＡｎｚｈｅｎ（Ｎｏ．２５２Ｈｏｓｐｉ...     

Assessment of the value of carcinoembryonic antigen reduction ratio as a prognosis factor in rectal cancer

Background

Carcinoembryonic antigen (CEA) is the most widely used tumor marker for colorectal cancer. This study aimed to investigate the role of CEA reduction ratio after preoperative chemoradiotherapy (CRT).

Methods

We enrolled 284 patients who underwent preoperative CRT followed by radical surgical resection. Patients were divided into 3 groups: serum CEA levels before CRT (pre-CRT CEA) less than 5 ng/mL (group 1); pre-CRT CEA of 5 ng/mL or more with CEA reduction ratio of 50% or more (group 2); and pre-CRT CEA of 5 ng/mL or more with CEA reduction ratio less than 50% (group 3).

Results

The 5-year disease-free survival (DFS) rate was not different between groups 1 (71.8%) and 2 (69.4%) but was signi?cantly lower in group 3 (49.5%). CEA group, lymph node status after CRT (ypN) stage, and histologic type were independent prognostic factors for DFS on multivariate analysis.

Conclusions

CEA reduction ratio might be an independent prognostic factor for DFS in rectal cancer patients treated with preoperative CRT and radical surgery.