盐酸万乃洛韦治疗单疱病毒性角膜炎临床疗效观察

运用盐酸万乃洛韦对单疱病毒性角膜炎３种临床类型进行对比治疗,并在树枝状或地图状上歧性角膜炎（定为Ｉ型）设置对照组。盐酸万乃洛韦每次３００ｍｇ,每天２次,疗程７ｄ,对３种临床类型角膜炎都有明显疗效,尤其对Ｉ型病变疗产更好,治愈率达９８．２％;与吗啉胍（病毒灵）比较,两者差异显著,盐酸万乃洛韦疗效明显优于吗啉胍（Ｐ〈０．０５）,且治疗后３个月、６个月复发次数与对照组比较明显减少（Ｐ〈０．０５）。     

重组人白细胞介素15表达载体的构建及其在大肠杆菌中的高效表达

目的 :获得重组人白细胞介素 1 5 (rhIL 1 5 )高效表达菌株。方法 :经细菌脂多糖 +γ干扰素活化的人外周血单个核细胞提取细胞总RNA ,用RT PCR方法扩增出编码人IL 1 5cDNA的基因片段 ,采用 pBV2 2 0表达载体 ,经DNA重组技术构建IL 1 5基因工程菌。结果 :核酸序列测定与预期一致 ,所表达的IL 1 5经SDS PAGE证明分子量约 1 5kD ,表达量占菌体总蛋白的 2 8% ,经CTLl2 细胞检测 ,表达产物粗提物 1∶1 0 0复性后效价可达到1 0 6IU /ml。结论 :构建的基因工程菌为IL 1 5高效表达菌株。     

水杨梅属植物化学成分及其生物活性的研究进展

Ｔｈｅｒｅａｒｅａｂｏｕｔ 70ｓｐｅｃｉｅｓｉｎｔｈｅｇｅｎｕｓＧｅｕｍ ,ｏｆｔｈｅｓｅ 6ｓｐｅｃｉｅｓａｒｅｆｏｕｎｄｉｎＣｈｉｎａ[1～ 4] .Ｓｏｍｅｓｐｅｃｉｅｓ ,ｓｕｃｈａｓＧ .ａｌｅｐｐｉｃｕｍＪａｃｑ ,Ｇ .ｊａｐｏｎｉｃｕｍＴｈｕｎｂ .ａｎｄＧ .ｊａｐｏｎｉｃｕｍＴｈｕｎｂ .ｖａｒ .ｃｈｉｎｅｎｓｅＢｏｌｌｅ ,ｈａｖｅｂｅｅｎｕｓｅｄｉｎｔｒａｄｉｔｉｏｎａｌＣｈｉｎｅｓｅｍｅｄｉｃｉｎｅａｎｄＪａｐａｎｅｓｅｆｏｌｋｍｅｄｉｃｉｎｅ .Ｔｈｅｙａｒｅａｐｐｌｉｅｄｔｏｒｅｍｏｖｅｔｏｘｉ…     

粉防己碱对大鼠肺动脉平滑肌细胞小电导钙激活钾通道的作用

目的　研究粉防己碱 (Tet)对大鼠肺动脉平滑肌细胞小电导钙激活钾通道 (SKCa)的作用。方法　内面朝外膜片箝单通道记录法。结果　Tet 7 5 μmol·L-1对电导值为 10pS的SKCa无明显影响 ,15 μmol·L-1可增加SKCa开放的概率 ,改变通道的开放和关闭模式 ,30 μmol·L-1降低通道的开放概率 ,通道开放以短暂簇状为主。结论　Tet对肺动脉平滑肌SKCa的作用与Tet的浓度有关 ,合适浓度下可增加通道的开放 ,K+ 外流增多 ,与Tet降低肺动脉张力有关。     

New 1-O-acyl alpha-L-rhamnopyranosides and rhamnosylated lactones from Streptomyces sp., inhibitors of 3 alpha-hydroxysteroid-dehydrogenase (3alpha-HSD)

Chemical screening with extracts of Streptomyces sp. (strain GT 61150) resulted in the detection, isolation, and structure elucidation of two new acyl alpha-L-rhamnopyranosides (1 and 2) and three new rhamnosyllactones A, B1 and B2 (3 approximately 5). Rhamnosyllactones B1 and B2 were obtained as a 5:1 mixture. The structures were confirmed by spectroscopic analysis, especially 2D-NMR techniques. The rhamnosyltransferase of our strain is able to connect the sugar moiety to heteroaromatic carboxylic acids and enols. The metabolites 1 and 4/5 as well as previously reported acylrhamnosides 6 approximately 11 inhibit the enzyme 3alpha-hydroxysteroid-dehydrogenase (3alpha-HSD).     

浆肌瓣覆盖吻合术在梗阻性大肠癌一期切除的应用

探讨浆肌瓣覆盖式吻合术在梗阻性大肠癌一期切除吻合中的应用价值。方法：１９９３年７月至１９９８年１１月采用该吻合术治疗梗阻性大肠癌２７例,其中急诊手术１１例。结果：全组无死亡病例及吻合口瘘的发生。结论：浆肌瓣覆盖式吻合术是治疗梗阻性大肠癌的较为理想的术式,可有效防止吻合口瘘。     

The treatment effects and mechanism of highly agglutinative staphylococci, local therapy in patients with superficial metastatic tumor

Highly agglutinative staphylococcin (HAS) is a new biological response modifier used in tumor treatment recently. It has being used in clinic as an effective drug. The superficial metastatic tumor is easy to treat and take samples, so we treated 35 cases of superficial metastatic tumor with HAS, observed the treatment effects and tried to seek the mechanism involved.Materials and methodsThirty-five cases with superficial metastatic tumor which diagnosed by pathology were treated in the Firs…     

广西某县原发性肝癌危险因素的非条件Logistic回归分析

目的：调查广西某县肝癌危险因素,为该县开展肝癌防治研究提供科学依据。方法：从该地区恶性肿瘤发病登记报告中,随机选择１９９６年～１９９７年肝癌患者３５０例为病例组：并随机选择１５２８例正常人群进行调查,为对照组;用非条件Ｌｏｇｉｓｔｉｃ回归模型进行多因素分析。结果有８个因素最终选入多因素非条件Ｌｏｇｉｓｔｉｃ回归模型,包括饮水类型,１０年前玉米月用量,心理情绪,ＨＢｓＡｇ和肝癌家庭史等。结论：广西某     

福耳心痛散对正常家猫心功能的影响

目的：观察福耳心痛散对正常家猫心功能的影响。方法：用ASU－DIC型彩色多普勒超声诊断仪观察测定给药前后家猫心功能。结果：福耳心痛散有明显增加正常家猫射血分数,每分心输量,左心室舒张末期容积的作用。结论：福耳心痛散有明显改善正常猫心功能的作用。     

Logistic逐步回归分析方法在模拟专家辩证诊断中的应用研究

目的：模拟专家辩证诊断的经验,便于编制电脑专家诊断系统。方法：运用现代多元统计Logistic逐步回归分析方法,按照诊断树的步骤计算出诊断树各枝杈和尾端各证型的诊断指数和Logistic回归方程。按诊断树各枝杈证型出现的概率连乘求出尾端证型（总的辩证分型）的概率。结果:Logistic逐步回归分析预测虚和实的结果与专家诊断的总符合率为88％;主证的总的符合率为93．8％;兼证的总符合率为79．7％。结论：Logistic逐步回归分析方法充分考虑了每一个变量和诊断树每一个枝杈对证的贡献度,符合专家临床逻辑思维过程和接近临床实际。