DNA Duplexes with Hydrophobic Modifications Inhibit Fusion between HIV-1 and Cell Membranes

Discovery of new drugs for the treatment of AIDS typically possessing unique structures associated with novel mechanisms of action has been of great importance due to the quick drug-resistant mutations of HIV-1 strains. The work presented in this report describes a novel class of DNA duplex-based HIV-1 fusion inhibitors. Hydrophobic groups were introduced into a DNA duplex skeleton either at one end, at both ends, or in the middle. These modified DNA duplexes inhibited fusion between HIV-1 and human cell membranes at micro- or submicromolar concentrations. Respective inhibitors adopted an aptamer pattern instead of a base-pairing interaction pattern. Structure-activity relationship studies of the respective DNA duplexes showed that the rigid and negatively charged DNA skeletons, in addition to the presence of hydrophobic groups, were crucial to the anti-HIV-1 activity of these compounds. A fluorescent resonance energy transfer (FRET)-based inhibitory assay showed that these duplex inhibitors interacted with the primary pocket in the gp41 N-terminal heptad repeat (NHR) instead of interacting with the lipid bilayers.     

侧卧位徒手气道开放置胃管法在重症脑卒中患者的应用

目的探讨适用于重症急性脑卒中昏迷伴舌后坠、颅内压增高患者的胃管置入方法。方法将90例重症急性脑卒中昏迷患者分为3组各30例,观察组采用侧卧位徒手开放气道法,对照1组采用侧卧位置管法,对照2组采用侧卧位拉舌法,比较3组患者1次置管成功情况、不良反应程度和舌损伤情况。结果1次置管成功率,观察组高于对照1组（P<0.01）,与对照2组比较差异无统计学意义（P>0.05）;不良反应程度,观察组低于对照2组（P<0.01）,与对照1组比较差异无统计学意义（P>0.05）;舌损伤情况,观察组低于对照2组（P<0.05）,与对照1组比较差异无统计学意义（P>0.05）。结论侧卧位徒手气道开放置胃管法,用于重症脑卒中昏迷伴舌后坠、颅内压增高患者,成功率高、刺激小、损伤轻,值得临床推广。     

泥灸治疗软组织疼痛病人的疗效观察及护理

软组织疼痛是由急性损伤后、慢性劳损、退行性病变、代谢物质滞留所致无菌性炎症刺激粘连压迫神经而引起。常用按摩、药物、局部封闭等方法来治疗,虽然有效,但疗效不持久,易短期复发,影响病人的生存质量。2009年我院疼痛科运用泥灸疗法配合针灸、拔火罐治疗软组织疼痛,并进行护理干预,疗效满意。     

Hapten Facilitates Food Allergen-Related Intestinal Hypersensitivity

BackgroundHaptens can bind to proteins to elicit antigenicity. Whether haptens play a role in the pathogenesis of food allergy remains to be investigated. This article aims to elucidate the role a hapten plays in food antigen-related T helper 2 (T_h2) pattern inflammation in the intestine.MethodsThe effect of trinitrobenzene sulfonic acid (TNBS; as a hapten) on the properties of dendritic cells was assessed by a cell culture model. BALB/c mice were sensitized with a mixture of TNBS and ovalbumin (OVA; as a model antigen). Intestinal T_h2 response, OVA-specific immunoglobulin E and histamine were analyzed with the mouse model. In addition to the infiltration of the intestinal inflammatory cells, cytokine expression profiles were determined.ResultsTNBS increased the expression of T-cell immunoglobulin and mucin domain-4 and CD80 and decreased the levels of interleukin-12 in dendritic cells. Higher serum levels of OVA-specific immunoglobulin E, histamine expression and skewed antigen-specific T_h2 polarization in the intestinal tissue were detected in mice sensitized with TNBS + OVA as compared with those treated with either OVA or TNBS alone. In addition, the TNBS-OVA-treated mice also showed an increased number of inflammatory cells, high levels of interleukin-4 and a decreased expression of interferon-γ in the lamina propria mononuclear cells.ConclusionsHapten TNBS can facilitate the initiation of food antigen-related T_h2 pattern inflammation, such as food allergy, in the intestine.     

槌果藤软膏治疗银屑病的实验研究

目的:观察槌果藤软膏对银屑病动物模型的疗效及其抗炎、止痒等作用。方法:观察计数小鼠尾部给药后鳞片表皮颗粒层形成情况;计数处于有丝分裂中期的阴道基底细胞有丝分裂数,并换算成有丝分裂指数;观察记录右旋糖苷致小鼠瘙痒次数及瘙痒持续时间;测定二甲苯致小鼠耳廓肿胀程度;测定小鼠腹腔毛细血管炎性渗出。结果:槌果藤软膏对小鼠尾部鳞片表皮颗粒层的形成具有显著的促进作用;可明显抑制小鼠阴道上皮细胞有丝分裂;能明显缓解右旋糖苷引起的小鼠瘙痒;减轻二甲苯致小鼠耳肿胀程度并抑制小鼠腹腔毛细血管通透性增高。结论:槌果藤软膏对小鼠银屑病模型有显著的治疗作用,并具有抗炎、止痒等作用。