缺血－再灌注兔心肌肌浆网自由基的测定

应用电子自旋共振波谱仪（ＥＳＲ）直接检测了缺血－再灌注兔心肌肌浆网自由基的变化，以探讨肌质网系统与氧自由基的关系。实验中将２０只兔随机分为再灌注对照组、超氧化物歧化酶（ＳＯＤ）组、ＡＴＰ－氯化镁组和人参皂甙Ｒｅ组。实验结果，ｇ值２．００４６处为半醌自由基波谱，其相对浓度各组依次为７８．９４±２．１２６，１４．４６±２．８６，２０．６５±７．６５，１４．６６±３．６７（ｘ±ＳＤ），对照组与用药组均有显著性差异（Ｐ＜０．０５），表明缺血－再灌注兔心肌肌浆网产生大量的自由基，用ＥＳＲ可以直接检测到半醌自由基，外源性高能磷酸盐制剂ＡＴＰ－氯化镁及人参皂甙Ｒｅ与超氧化物歧化酶一样，发挥清除兔心肌肌浆网自由基的作用。     

术中使用缓释型5-FU对于进展期结肠癌的疗效研究

随着生活水平的提高,恶性肿瘤的发病率也逐年增高,最新资料统计表明,目前全球结肠直肠癌病人每年有100万之多[1]。在我国其发病率近年来也呈明显上升趋势,到目前为止,结肠直肠癌病人的术后5年生存率也一直徘徊在50%左右[2]。因而如何有效地进行结肠肿瘤治疗,无论是在手术还是在其他辅助治疗方面都值得我们深入地探讨和研究。     

Treatment of severe subglottic laryngotracheal stenosis using hyoid graft with sternohyoid muscle flap]

OBJECTIVE: To evaluate the possibility and reliability of the hyoid-sternohyoid graft transfer in the correction of server subglottic laryngotracheal stenosis, and delineate the operation skills and clinical results. METHODS: Seven patients with severe subglottic stenosis underwent laryngotracheal reconstruction using the hyoid grafts with sternohyoid muscle flaps (HG-SHMF). Five of these patients had traumatic subglottic stenosis, one with scar tissue of unknown etiology arising in the subglottic region, another with tracheal narrowing caused by inhalation of hydrochloric acid. RESULTS: All seven patients were successfully decannulated with moderate good voice. The average time from reconstruction to decannulation was 15.4 months. The stent was endoscopically removed with a range of 3 to 22 months; the mean time required for stenting was 9.6 months. Two patients who received additional salvage reconstruction procedures because of graft or stent displacement were extubated with improved voices and satisfactory airway. CONCLUSIONS: The HG-SHMF transfer was a single-stage reconstruction, relatively simple procedure that can restore an adequate airway and a good voice. Patients undergoing laryngotracheal reconstruction with HG-SHMF must have regular, long-term follow-up since graft displacement and recurrent granulation tissue or scar reformation can cause restenosis after an initially successful surgery. This procedure should be used in a large number of patients to further test its reliability.     

地震伤患者医院感染目标性监测

对某院收治的50名地震伤员进行医院感染目标性监测，有效地预防与控制医院感染的发生。通过分类安置伤员，专人目标监测，严格执行消毒隔离制度及手卫生、标准预防措施等，将医院感染发生率降至最低：仅1例重症挤压伤者发生医院感染，医院感染率为2．00％。     

Genetic changes in JC virus possibly associated with progressive multifocal leukoencephalopathy]

Progressive multifocal leukoencephalopathy (PML) is a fetal demyelinating disease in the central nervous system. PML was once a rare disease, but it is now relatively common among AIDS (acquired immunodeficiency syndrome) patients. The immunological state of patients mainly contributes to the pathogenesis of PML. Genetic changes of the etiological agent, however, may also be involved in the development and progression of the disease. The major genetic changes possibly associated with PML include the regulatory region rearrangement and the VP1 loop mutation. Both changes have been identified as genetic changes usually occurring in JCV (JCvirus) DNAs from the brain and cerebrospinal fluid of PML patients. Although it remained to be clarified how these changes are involved in the pathogenesis of PML, accumulating evidence suggests that the VP1 loop mutation is associated with the progression of PML. Here we overview studies (mainly those performed by ourselves) on these genetic changes.     

大鼠胼胝体神经元化学性质研究:Ⅰ.谷氨酸的免疫细胞化学研究

采用辣根过氧化物酶(HRP)逆行示踪与谷氨酸免疫细胞化学结合法对大鼠额叶和枕叶皮质内胼胝体神经元的化学性质进行了研究.结果发现含谷氨酸的胼胝体神经元为大中型锥体细胞,主要分布于皮质Ⅱ/Ⅲ、Ⅴ和Ⅵ层,并有成群分布的倾向.含谷氨酸的胼胝体神经元的数目约占 HRP 标记的胼胝体神经元的17%(额叶)和29%(枕叶).这一结果从形态学上证实,大鼠大脑皮质内相当一部分胼胝体神经元使用谷氨酸作为兴奋性递质。     

微波快速过氧化物酶染色法与灌注法用于微循环形态学研究之比较

以健康Ｗｉｓｔａｒ大白鼠为材料，对微波快速内源性过氧化物酶染色与灌注法显示微血管的方法进行了对比研究。结果，过氧化物酶组织化学法具有不用灌注，操作简单，所用时间短，对血管无扩张、破裂等人为改变的特点，保持了微血管真实的自然形态和管径大小，可以定量或半定量地判定组织器官活体时的血液循环状况。可以用于人及动物的大脑、脊髓、皮肤、耳及食管等组织内微血管形态学研究和定量分析。并对过氧化物酶显示微血管的原理和微波辐射促进染色的原理及特点进行了分析、讨论。     

胰RNA对离体瘤细胞的杀伤作用及其机制的探讨

在体外培养瘤细胞的实验中,胰RNA提取物对艾氏腹水瘤细胞及其他瘤细胞(S_(180),P_(88),H_(22))有杀伤作用。在胰RNA提取物中,对瘤细胞具有杀伤作用的活性物质不是性凝集素样多糖类物质或能透过半透膜的小分子化合物,而是具有二级结构的低分子RNA。     

Mitogenic effect of polyclonal anti-porcine lymphocyte E-receptor antiserum IgG

In the present paper we report the mitogenic effect of the sheep anti-porcine lymphocyte E-receptor antiserum IgG(APERIgG) on porcine peripheral T-lymphocytes in vitro. The effect was apparently dose-dependent and not seen in the B-lymphocyte cultures. Kinetic study showed that the maximal 3H-thymidine incorporation, similar to that induced by Con A, occurred on the third day of incubation. Neither F(ab')2 nor Fab fragment of APERIgG was able to induce cell proliferation, indicating that the integrity of IgG molecule was essential for cell response. It was very interesting to note that though there is cross antigenicity between porcine and human E-receptors, no similar effect could be produced by APERIgG in human peripheral lymphocyte cultures under the same experimental conditions, suggesting the existence of minor difference in structure between the porcine and human E-receptors.